Enhancement of Annexin V in response to combination of epigallocatechin gallate and quercetin as a potent arrest the cell cycle of colorectal cancer

Abstract

Colorectal cancer (CRC) is one of the most common cancers leading to comorbidities and mortalities globally. The rational of current study was to evaluate the combined epigallocatechin gallate and quercetin as a potent antitumor agent as commentary agent for therapeutic protocol. The present study investigated the effect of epigallocatechin Gallate (EGCG) (150mg) and quercetin (200mg) at different proportions on proliferation and induction of apoptosis in human colon cancer cells (HCT-116). Cell growth, colonogenic, Annexin V in addition cell cycle were detected in response to phytomolecules. Data obtained showed that, the colony formation was inhibited significantly in CRC starting from the lowest concentration tested of 10 µg/mL resulting in no colonies as visualized by a phase-contrast microscope. Data showed a significant elevation in the annexin V at 100 µg/mL EGCG(25.85%) and 150 µg/mL quercetin (48.35%). Moreover, cell cycle analysis showed that this combination caused cell cycle arrest at the G1 phase at concentration of 100 µg/mL (72.7%) and 150 µg/mL (75.25%). The combined effect of epigallocatechin Gallate and quercetin exert antiproliferative activity against CRC, it is promising in alternative conventional chemotherapeutic agent.

Annexin A protein family: Focusing on the occurrence, progression and treatment of cancer

“…In oncogenic human papillomavirus-positive non-small cell lung cancer, ANXA4 is mediated by HPV16E7 and HPV16E6/E7, promotes cell proliferation and regulates the cell cycle and mitosis (Ciotti et al, 2009). Meanwhile, the combination of epigallocatechin gallate and quercetin enhance ANXA5 to effectively inhibit colorectal cancer arrest at the G 1 stage (Xue et al, 2009;Al-Ghamdi et al, 2021). Furthermore, the overexpression of ANXA9 has been found to increase the migration of gastric cancer and form larger and more cell clones, which decrease in the G 1 phase and increase in the S and G 2 phases (Zhou et al, 2021b).…”

Application of Quercetin in the Treatment of Gastrointestinal Cancers

“…Ghamedi et al evaluated the mechanism and effects of a 200 mg dose of quercetin and a 150 mg dose of EGCG (epigallocatechin gallate) at varying proportions on the induction of apoptosis and inhibition of proliferation of human CRC cell line HCT-116 ( Al-Ghamdi et al, 2021 ). The phytomolecules inhibited cell growth, arrested cell cycle, annexin V, and reduced clonogenicity.…”

“…The combination therapy induced cell cycle arrest at the G1 phase. In conclusion, the combination of EGCG plus quercetin could be used as effective combination chemotherapy in the future, but more studies are required to establish a suitable dose and any side effects ( Al-Ghamdi et al, 2021 ).…”

Antarctic Macroalgae <i>Palmaria decipiens</i> (Rhodophyta, Palmariales) Extracts Present Antioxidant and Antitumor Activity against Colorectal Cancer

“…Studies have shown that phenolic compounds such as gallic acid has significant biological properties, directly affecting the proliferation and replication of tumor cells, acting as antimutagenic and antioxidant agents, being considered effective against cancer cells [26] [27] [28]. Another phenolic derivate is quercetin, considered an inhibitor of the development of different types of cancer, since this flavonoid can affect cell proliferation, cell cycle regulation and usually participate in multiple signaling pathways [26] [29] [30]. A third flavonoid identified in this study is kaempferol, that has also been reported to participate in biological properties such as anti-inflammatory, antioxidant and antimutagenic, reducing the risk of cancer and other diseases [25] [31] [32].…”