Inheritance of seedlessness and the molecular characterization of the INO gene in Annonaceae

Nassau, B R R M; Mascarenhas, Paulo; Guimarães, Amanda Gonçalves; Feitosa, Fabiana; Ferreira, Helena; Castro, B. M. C.; Zanuncio, José Cola; Costa, Márcia Regina; Nietsche, Sílvia

doi:10.1590/1519-6984.246455

Cited by 3 publications

(7 citation statements)

References 17 publications

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“…Lora et al (2011) designed primers within, or closely flanking the transcribed region of INO for detection of the wild-type locus, and demonstrated amplification from wild-type A. squamosa, A. cherimola , and four other species that cover the phylogenetic range of the genus Annona . These primers were confirmed to function as expected in our A. squamosa varieties (Nassau et al, 2021 and this work). Notably the primer pairs only allow detection of the wild-type locus and a homozygous mutant could only be detected by the absence of amplification.…”

Section: Discussionsupporting

confidence: 87%

“…Results showed that the absence of seeds was also associated with failure in the development of the external integument of the ovule and in the development of seeds, similar to that observed in Ts (Mendes et al, 2012; Santos et al, 2014). In addition, preliminary studies of inheritance of the stenospermocarpic absence of seeds involving F1 progenies in Bs ( A. squamosa wild type x Bs) also indicated the same expected recessive nature for the mutation as in Ts (Nassau et al, 2021). However, it was not known whether the molecular basis of the INO deletion described is widespread in A. squamosa and may be responsible for the case of aspermia described in Bs.…”

Section: Introductionmentioning

confidence: 92%

“…In addition, preliminary studies of inheritance of the stenospermocarpic absence of seeds involving F 1 progenies in Bs (A. squamosa wild type x Bs) also indicated the same expected recessive nature for the mutation as in Ts (Nassau et al, 2021). However, it was not known whether the molecular basis of the INO deletion described is widespread in A. squamosa and may be responsible for the case of aspermia described in Bs.…”

Section: Introductionmentioning

confidence: 99%

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Seedless fruit inAnnona squamosaL. is monogenic and conferred byINOlocus deletion in multiple accessions

Rodrigues

Gasser

Pimenta

et al. 2022

Preprint

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Understanding the genetic basis and inheritance of a trait facilitates the planning of breeding and development programs of new cultivars. In the sugar apple tree (Annona squamosa L.), the mechanism of the desirable seedless trait in the Thai seedless (Ts) and Brazilian seedless (Bs) accessions was associated with a deletion of the INNER NO OUTER (INO) locus. Genetic analysis of F1, F2 and backcross descendants of crosses of Bs to fertile wild-type varieties showed that seedlessness was recessive and monogenic. Whole genome sequencing of a third accession, Hawaiian seedless (Hs), identified a 16 kilobase deletion including INO. The finding of an identical deletion in Ts and Bs indicated a common origin among genotypes, from a single deletion event. Analysis of microsatellite markers could not preclude the possibility that all three accessions are vegetatively propagated clones. The sequence of the deletion site enabled formulation of a codominant assay for the wild-type and mutant genes that validated the INO gene deletion as the cause of seedless trait, and can be used in the selection of new seedless varieties. The study findings and obtained progenies should be useful in breeding and introgression programs of the trait into elite sugar apple lines and into other Annonas by means of interspecific crossings.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Seedless fruit inAnnona squamosaL. is monogenic and conferred byINOlocus deletion in multiple accessions

Rodrigues

Gasser

Pimenta

et al. 2022

Preprint

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“…A related study showed the molecular basis for the absence of seeds in Thai seedless (Ts), an A. squamosa cultivar, resulting in the development of a set of LMINO primers (Lora et al 2011). The use of LMINO primers in the mutant Bs were validated in another study, justifying the molecular basis for INO locus deletion (Nassau et al 2023). The authors also suggested that the inheritance of the presence/absence of seeds in A. squamosa is probably controlled by only a single dominant gene, whereas the recessive condition determines the absence of seeds in fruits.…”

Section: Introductionmentioning

confidence: 97%

“…Brazilian seedless (Bs) is a mutant of Annona squamosa with a stenospermocarpy phenotype and was originally identified in northeast Brazil. It was first described in 1940 in the state of São Paulo and was used for intraspecific crossings to develop new seedless sugar apple cultivars with combined desirable quality attributes (Cunha 1953, Nassau et al 2023. Interspecific hybridizations were also carried out between the mutant Bs and the atemoya 'Gefner' (A. cherimola Mill.…”

Section: Introductionmentioning

confidence: 99%

Molecular marker-assisted selection for seedlessness in atemoya breeding

Rodrigues

Nietsche

Pimenta

et al. 2022

Crop Breed. Appl. Biotechnol.

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Perennial plant breeding is an expensive and time-consuming process, mainly due to the extended growth time for juveniles. In these cases, the use of molecular marker-assisted selection (MMAS) allows for the selection of a characteristic of interest at the seed or seedling stages. The objective of this work was to characterize the segregation of the INO locus and to use MMAS for the early selection of seedless genotypes of atemoya 'Gefner' [(G; Annona cherimola Mill. × Annona squamosa L.) × Brazilian seedless (Bs; Annona squamosa)]. After primer validation and MMAS, 24 plants of the F 2 population were selected and designated as candidate genotypes for the absence of seeds, as INO alleles were absent in them. For further studies on breeding programs for this species, 53 heterozygous seedlings were considered as genetic resources during selection.

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Seedless fruit in Annona squamosa L. is monogenic and conferred by INO locus deletion in multiple accessions

et al. 2023

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Key message Inheritance of the presence/absence of seeds in Annona squamosa is mediated by a single fully recessive gene and is caused by a deletion of the INNER NO OUTER (INO) locus. Abstract For some fruits, seedless varieties are desirable for consumption and processing. In the sugar apple tree (Annona squamosa L.), the seedless trait in the Thai seedless (Ts) and Brazilian seedless (Bs) accessions was associated with defective ovules and an apparent deletion of the INNER NO OUTER (INO) ovule development gene locus. Segregation analysis of F2 and backcross descendants of crosses of Bs to fertile wild-type varieties in this species with a multi-year generation time showed that seedlessness was recessive and controlled by a single locus. Comparison of whole genome sequence of a wild-type plant and a third accession, Hawaiian seedless (Hs), identified a 16 kilobase deletion including INO in this line. Ts and Bs lines were shown to have an identical deletion, indicating a common origin from a single deletion event. Analysis of microsatellite markers could not preclude the possibility that all three seedless accessions are vegetatively propagated clones. The sequence of the deletion site enabled a codominant assay for the wild-type and mutant genes allowing observation of complete cosegregation of the seedless/defective ovule phenotype with the INO deletion, showing maximal separation of less than 3.5 cM. The observed deletion is the only significant difference between the wild-type and Hs line over 587 kilobases, likely encompassing much more than 3.5 cM, showing that the deletion is the cause of seedless trait. The codominant markers and obtained progenies will be useful for introgression of the seedless trait into elite sugar apple lines and into other Annonas through interspecific crossings.

show abstract

Inheritance of seedlessness and the molecular characterization of the INO gene in Annonaceae

Cited by 3 publications

References 17 publications

Seedless fruit inAnnona squamosaL. is monogenic and conferred byINOlocus deletion in multiple accessions

Seedless fruit inAnnona squamosaL. is monogenic and conferred byINOlocus deletion in multiple accessions

Molecular marker-assisted selection for seedlessness in atemoya breeding

Seedless fruit in Annona squamosa L. is monogenic and conferred by INO locus deletion in multiple accessions

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