Antimitotic and antimutagenic action of the Hymenaea stigonocarpa bark on dividing cells

Abstract

The objective of this study was to evaluate the action of Hymenaea stigonocarpa bark hydroalcoholic extract against a mutagenic compound using A. cepa meristematic root cells as a test system. The treatment groups were: Negative Control (NC) -distilled water; Positive Control (PC) -paracetamol at a concentration of 0.008 mg/mL, Jatoba Control (JC) -aqueous fraction jatobá-do-cerrado at 0.5 or 1.0 or 1.5 mg/mL, and Simultaneous Treatment (ST) -jatobá-do-cerrado aqueous fraction at a concentration of 0.5 or 1.0 or 1.5 mg/mL associated with paracetamol solution at a concentration of 0.008 mg/mL. All groups were analyzed at 24 and 48 h. Five onion bulbs (five replications) were used for each treatment group. The root tips were fixed in Carnoy and slides prepared by the crush technique. Cells were analyzed throughout the cell cycle, totaling 5,000 for each treatment group at each exposure time. Mitotic indices were subjected to statistical analysis using the chi-square test (p<0.05). From the results it was found that the ST group, at the three concentrations, significantly potentiated the antiproliferative effect of the test system cells when compared to PC, NC and TJ at the three concentrations. Furthermore, the three ST concentrations significantly reduced the number of cell aberrations when compared to the number of aberrant cells obtained for the PC, demonstrating antimutagenic action on the A. cepa test system cells.Keywords: jatobá-do-cerrado, cytotoxicity, protector effect. Ação antimitótica e antimutagênica do ritidoma de Hymenaea stigonocarpa Mart ex Hayne na divisão celularResumo O objetivo do presente trabalho foi avaliar a ação do extrato hidroalcólico do ritidoma de Hymenaea stigonocarpa frente a um composto mutagênico, utilizando como sistema teste as células meristemáticas de raízes de A. cepa. Os grupos tratamentos avaliados foram: Controle Negativo (CN) -água destilada; Controle Positivo (CP) -paracetamol na concentração de 0,008 mg/mL, Controle Jatobá (CJ) -fração aquosa de jatobá-do-cerrado na concentração de 0,5 ou 1,0 ou 1,5 mg/mL, e Tratamento Simultâneo (TS) -fração aquosa de jatobá-do-cerrado na concentração de 0,5 ou 1,0 ou 1,5 mg/mL associada a solução de paracetamol na concentração de 0,008 mg/mL. Todos os grupos foram analisados nos tempos de 24 e 48 h. Para cada grupo tratamento cinco bulbos de cebolas (cinco repetições) foram utilizados. As radículas foram fixadas em Carnoy e as lâminas preparadas pela técnica de esmagamento. Analisaram-se células em todo ciclo celular, totalizando 5.000 para cada grupo tratamento em cada tempo de exposição. Os índices mitóticos obtidos foram submetidos à análise estatística do 05). A partir dos resultados verificou-se que o grupo TS, nas três concentrações, potencializou o efeito antiproliferativo significativo as células do sistema teste quando comparado ao CP, CN e TJ nas três concentrações. Ainda, o TS nas três concentrações reduziu de forma significativa o número de aberrações celulares quando comparado com o número de células aberrantes ob...

Medicinal plants profile used by the 3rd District population of Maceió-AL

“…This is due to the cat that the interpretation of this concept represents for a share of the population, the absence of chemicals, which would be those that can cause some damage or otherwise represent danger to people's health (Vieira et al, 2010;Santana et al, 2016).…”

Cytogenotoxicity of Shakes Added with Synthetic Micro Ingredients

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Acute Toxicity of Grape, Plum and Orange Synthetic Food Flavourings Evaluated in in vivo Test Systems

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“…Root meristem cells of Allium cepa L. (onion) are an effi cient test system for initial screening of acute cytotoxicity and genotoxicity of chemicals (14,15). This bioassay provides excellent proliferation kinetic properties and a few chromosomes (2n=16), which facilitates the detection of mitotic spindle changes and chromosomal breaks (16,17).…”