Evaluation of eight protocols for genomic DNA extraction of Hypostomus commersoni Valenciennes, 1836 (Loricariidae: Siluriformes)

Mezzomo, Priscila; Mielniczki-Pereira, Albanin Aparecida; Sausen, Tanise Luisa; Marinho, Jorge Reppold; Cansian, Rogério Luís

doi:10.1590/1519-6984.229278

Cited by 4 publications

(3 citation statements)

References 35 publications

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“…Genetic studies required good quality DNA. Isolated DNA concentration and purity depends upon its isolation technique [1]. Mostly soft tissues i.e., muscles, liver, heart and gills are selected for isolation of DNA [15].…”

Section: Resultsmentioning

confidence: 99%

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Comparison of seven genomic DNA isolation techniques from internal organs, gills and muscle tissues of Notopterus notopterus (Notopteridae) using PCR amplification and Nanodrop

Ahmad

2023

ijbch

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The techniques and the principles used in DNA isolation play a vital role in the obtaining of a purified genetic material. In present study, we investigated the efficiency of seven genomic DNA isolation techniques in terms of isolated DNA concentration, yield and purity from internal organs, gills and muscle tissues of Notopterus notopterus. Isolated DNA quality was analysed through Nanodrod and PCR using mitochondrial COI genetic markers. Results showed that GeneJET Genomic DNA Purification Kit was found significantly higher in terms of isolated DNA concentration (1200-1288 ng.ul -1 ), yield (257.6 ng.ul -1 ) and purity (1.91-2.00), and also successful in PCR amplification as compared to other evaluated six traditional DNA isolation methods. The nucleotide minimum bands range was observed 200 base pairs in heart sample and maximum 600 base pair was observed in intestine. There is no data on description of parameters analysed in this work to date, neither the evaluation of isolated DNA using PCR amplification of mitochondrial COI gene for the species N. notopterus. Present study also revealed that the traditional DNA isolation methods are the secondary choice for isolation of DNA. The data of present study also indicated that the GeneJET Genomic DNA Purification Kit is useful for DNA isolation and can be used best in genetic applications for fishes.

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Section: Resultsmentioning

confidence: 99%

“…From a small piece of tissue, isolation of high-quality DNA is difficult. Therefore, the DNA isolation method must be accurate, fast, effective, and productive with less contamination [1]. It must be economical based on costs and time.…”

Section: Introductionmentioning

confidence: 99%

Comparison of seven genomic DNA isolation techniques from internal organs, gills and muscle tissues of Notopterus notopterus (Notopteridae) using PCR amplification and Nanodrop

Ahmad

2023

ijbch

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“…However, the purity of isolated DNA was 1.21 A260/280. The purity was lower than optimal as it is considered that the optimal purity range is from 1.7 to 2.0 А260/280 (MEZZOMO et al, 2020).…”

Section: Yield and Purity Of Genomic Dna From Urnula Mediterraneamentioning

confidence: 99%

DNA-based molecular identification of Urnula mediterranea (Ascomycota, Pezizales) collected in Central Serbia

Arsenijević¹,

Blagojević²,

Planojević³

et al. 2021

Kragujevac J Science

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Molecular systematics is a branch of systematics that analyzes genetic markers for the classification of organisms. Genetic markers include target sequences of molecules DNA, RNA, and amino acids. The most often used genetic markers are DNA markers, localized on DNA molecules. The study aim was DNA-based molecular identification of species Urnula mediterranea (M. Carbone, Agnello & Baglivo) M. Carbone, Agnello & P. Alvarado (2013), by using molecular systematics methods. This species was recently discovered for the first time in Central Serbia and determined only based on morphological characteristics. For the first time, U. mediterranea sampled in Serbia, was identified with DNA molecular markers. Extraction of DNA molecules was performed from the fruiting body of U. mediterranea. ITS region and the part of the gene for 28S rRNA were amplified and sequenced by the Sanger method. Comparing the analyzed sequences with the sequences from the database, a match of 99.72% was found for the ITS region and 100% for the part of the gene for 28S rRNA. By molecular identification, it has been established that the sample belongs to the species U. mediterranea. Both analyzed DNA sequences of U. mediterranea were deposited into the NCBI database.

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An efficient, fast and inexpensive method for genomic DNA extraction of fish tissue

2023

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Evaluation of eight protocols for genomic DNA extraction of Hypostomus commersoni Valenciennes, 1836 (Loricariidae: Siluriformes)

Cited by 4 publications

References 35 publications

Comparison of seven genomic DNA isolation techniques from internal organs, gills and muscle tissues of Notopterus notopterus (Notopteridae) using PCR amplification and Nanodrop

Comparison of seven genomic DNA isolation techniques from internal organs, gills and muscle tissues of Notopterus notopterus (Notopteridae) using PCR amplification and Nanodrop

DNA-based molecular identification of Urnula mediterranea (Ascomycota, Pezizales) collected in Central Serbia

An efficient, fast and inexpensive method for genomic DNA extraction of fish tissue

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