Chemical composition of essential oils from the apiaceae family, cytotoxicity, and their antifungal activity in vitro against candida species from oral cavity

Abstract

The aims of this research were: evaluate the chemical composition and the cytotoxicity of the Cuminum cyminum (cumin), Anethum graveolens (dill), Pimpinella anisum (anise) and Foeniculum vulgare (fennel) essential oils, as well as their antifungal activity in vitro against ten Candida spp. isolates. The chemical composition of the oils was analyzed by means of gas chromatography coupled with mass spectrometry (GC/MS). The cytotoxicity assays were performed, using the cell proliferation reagent WST-1 in L929 mouse fibroblasts (20x10 3 well -1 ). The determinate the Minimum Inhibitory Concentration (MIC), was performed through the Broth Microdilution technique (CLSI). The chemical main components were the cuminaldehyde (32.66%) for cumin, carvone (34.89%) for the dill, trans-anethole (94.01%) for the anise and anethole (79.62%) for the fennel. Anise and fennel did not were cytotoxic in all the tested concentrations, however the cumin oil was cytotoxic in the concentration of 20 mg.mL -1 and the dill in the concentrations of 20 and 8 mg.mL -1 . All yeasts were susceptible against the evaluated essential oils. Cumin presented the lowest MIC against yeasts. We concluded that all the essential oils presented inhibitory action against Candida spp., and C. cyminum, P. anisum and F. vulgare were not cytotoxic in the same minimum inhibitory concentrations for the fungi. Composição química de óleos essenciais da família apiaceae, citotoxicidade e sua atividade antifúngica in vitro contra espécies de candida da cavidade oral ResumoOs objetivos desta pesquisa foram: avaliar a composição química e a citotoxicidade dos óleos essenciais de Cuminum cyminum (cominho), Anethum graveolens (endro), Pimpinella anisum (erva-doce) e Foeniculum vulgare (funcho), bem como sua atividade antifúngica in vitro contra dez isolados de Candida spp.. A composição química dos óleos foi analisada por meio de cromatografia gasosa acoplada à espectrometria de massa (GC / MS). Os ensaios de citotoxicidade foram realizados, utilizando o reagente de proliferação celular WST-1 em fibroblastos de ratinho L929 (20x103 poço-1). A determinação da Concentração Inibitória Mínima (MIC) foi realizada através da técnica de microdiluição em caldo (CLSI). Os principais componentes químicos foram o cuminaldeído (32.66%) para cominho, carvona (34.89%) para o endro, trans-anetol (94.01%) para erva-doce e anetol (79.62%) para a funcho. O endro e a erva-doce não foram citotóxicos em todas as concentrações testadas, no entanto, o óleo de cominho foi citotóxico na concentração de 20 mg.mL -1 e o endro nas concentrações de 20 e 8 mg.mL -1 . Todas as leveduras foram suscetíveis aos óleos essenciais avaliados. O cominho apresentou a menor CIM contra as leveduras. Concluímos que todos os óleos essenciais apresentaram ação inibidora contra Candida spp., e C. cyminum, P. anisum e F. vulgare não foram citotóxicos nas mesmas concentrações inibitórias mínimas para os fungos.Palavras-chave: Cuminum cyminum, Candidíase atrófica crônica, Candida spp., antifúngico.

Determination of the Elemental Composition of Aromatic Plants Cultivated Industrially in the Republic of Moldova Using Neutron Activation Analysis

“…A large body of research has been dedicated to studying the essential oils compositions of the species under discussion [8][9][10][11]. They have been popularly used in pharmaceuticals, medicine, cosmetic products, the tobacco industry, agriculture, and perfumery; for aromatherapy; food preparation, preservation, and flavoring; and as melliferous and ornamental plants [12][13][14][15][16][17][18][19][20].…”

Insecticidal effects of some essential oils against box tree moth (Cydalima perspectalis Walker (Lepidoptera: Crambidae))

“…Major constituents of the essential oils of test plants has been previously reported by, Kordali et al (2006); Tozlu et al (2011);Carroll et al (2017); Duran and Kaya (2018); Amor et al ( 2019); Vieira et al (2019); Montenegro et al (2020); Paiano et al (2020). A list of the constituents and grouped components of this essential oils are presented in Table 1.…”

Characteristics of Essential Oils of <i>Apiaceae </i>Family: Their Chemical Compositions, <i>in vitro </i>Properties and Effects on Broiler Production

“…Among the tested fungi, A. flavus was the most sensitive (more than 80% ZOI) to DEO at 10 µ L, followed by the other tested fungi. More recently, ten Candida species were examined against DEO and found very significant fungicidal effects with a MIC of 8.75 mg/mL for all tested fungi ( Vieira et al , 2019 ).…”