First report of the parasitoid Pachycrepoideus vindemmiae (Rondani, 1875) (Hymenoptera: Pteromalidae) parasitizing Synthesiomyia nudiseta (Van der Wulp, 1883) (Diptera: Muscidae)

Marchiori, Carlos Henrique; Borges, Lígia Miranda Ferreira

doi:10.1590/1519-6984.03516

Cited by 8 publications

(7 citation statements)

References 9 publications

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“…To date, venom proteins of endoparasitoids are relatively well studied (Labrosse et al, 2005a,b;Colinet et al, 2009;Furihata et al, 2013;Goecks et al, 2013;Heavner et al, 2013;Mortimer et al, 2013). However, the venom protein repertoire of ectoparasitoids is less well known (Marchiori and Barbaresco, 2007;Marchiori et al, 2013;Chen et al, 2015;Marchiori and Borges, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Pachycrepoideus vindemmiae belongs to the family Pteromalidae (Hymenoptera). It is a versatile and solitary pupal ectoparasitoid that parasitizes various flies, including the genera of Drosophila, Musca, Anastrepha, Calliphora, and so on (Marchiori and Barbaresco, 2007;Marchiori et al, 2013;Marchiori and Borges, 2017). In this study, we examined the diversity of P. vindemmiae venom compositions based on both transcriptome-sequencing and proteome analysis, and compared it to venoms of three parasitoid families.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Comparative Analysis of Venom Proteins in a Pupal Ectoparasitoid, Pachycrepoideus vindemmiae

Yang

Liu

et al. 2020

Front. Physiol.

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Parasitoid wasps inject venom containing complex bioactive compounds to regulate the immune response and development of host arthropods and sometime paralyze host arthropods. Although extensive studies have been conducted on the identification of venom proteins in larval parasitoids, relatively few studies have examined the pupal parasitoids. In our current study, a combination of transcriptomic and proteomic methods was used to identify 64 putative venom proteins from Pachycrepoideus vindemmiae, an ectoparasitoid of Drosophila. Expression analysis revealed that 20 tested venom proteins have 419-fold higher mean expression in the venom apparatus than in other wasp tissues, indicating their specialization to venom. Comparisons of venom proteins from P. vindemmiae and other five species spanning three parasitoid families detected a core set of "ancient" orthologs in Pteromalidae. Thirty-five venom proteins of P. vindemmiae were assigned to the orthologous groups by reciprocal best matches with venoms of other pteromalids, while the remaining 29 were not. Of the 35 categories, twenty-seven have orthologous relationships with Nasonia vitripennis venom proteins and 25 with venoms of Pteromalus puparum. More distant relationships detected that five and two venom proteins of P. vindemmiae are orthologous with venoms of two Figitidae parasitoids and a Braconidae representative, respectively. Moreover, twenty-two venoms unique to P. vindemmiae were also detected, indicating considerable interspecific variation of venom proteins in parasitoids. Phylogenetic reconstruction based on a set of single-copy genes clustered P. vindemmiae with P. puparum, N. vitripennis, and other members of the family Pteromalidae. These findings provide strong evidence that P. vindemmiae venom proteins are well positioned for future functional and evolutionary studies.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and Comparative Analysis of Venom Proteins in a Pupal Ectoparasitoid, Pachycrepoideus vindemmiae

Yang

Liu

et al. 2020

Front. Physiol.

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“…Pachycrepoideus vindemmiae (Hymenoptera: Pteromalidae) is a versatile and solitary pupal ectoparasitoid of many flies whose hosts range from Drosophilidae to Anthomyiidae, Calliphoridae, Muscidae, Sarcophagidae, Tachinidae, Tephritidae, and so on (Marchiori and Borges, 2017). Unlike the multiple virulence factors in many koinobiont parasitoids, venom is the only required parasitic factor for successful parasitism of P. vindemmiae.…”

Section: Introductionmentioning

confidence: 99%

The Pupal Ectoparasitoid Pachycrepoideus vindemmiae Regulates Cellular and Humoral Immunity of Host Drosophila melanogaster

et al. 2019

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The immunological interaction between Drosophila melanogaster and its larval parasitoids has been thoroughly investigated, however, little is known about the interaction between the host and its pupal parasitoids. Pachycrepoideus vindemmiae, a pupal ectoparasitoid of D. melanogaster, injects venom into its host while laying eggs on the puparium, which regulates host immunity and interrupts host development. To resist the invasion of parasitic wasps, various immune defense strategies have been developed in their hosts as a consequence of co-evolution. In this study, we mainly focused on the host immunomodulation by P. vindemmiae and thoroughly investigated cellular and humoral immune response, including cell adherence, cell viability, hemolymph melanization and the Toll, Imd, and JAK/STAT immune pathways. Our results indicated that venom had a significant inhibitory effect on lamellocyte adherence and induced plasmatocyte cell death. Venom injection and in vitro incubation strongly inhibited hemolymph melanization. More in-depth investigation revealed that the Toll and Imd immune pathways were immediately activated upon parasitization, followed by the JAK/STAT pathway, which was activated within the first 24 h post-parasitism. These regulatory effects were further validated by qPCR. Our present study manifested that P. vindemmiae regulated the cellular and humoral immune system of host D. melanogaster in many aspects. These findings lay the groundwork for studying the immunological interaction between D. melanogaster and its pupal parasitoid.

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“…Pachycrepoideus vindemiae belongs to the Pteromalidae (Hymenoptera) and is a solitary generalist that attacks various fly pupae, including Anthomyiidae, Calliphoridae, Drosophilidae, Muscidae, Sarcophagidae, Tachinidae, Tephritidae, and so on (Marchiori & Barbaresco, 2007; Marchiori & Borges, 2017; Marchiori, 2000; Marchiori, Borges, & Ferreira, 2013; Marchiori, Pereira, & Filho, 2003). Our previous study reported that P. vindemiae venom proteins strongly inhibited the hemolymph melanization of host Drosophila melanogaster (Yang et al, 2019), and SPIs are one of the most commonly abundant components in wasp venoms (Poirié, Colinet, & Gatti, 2014).…”

Section: Introductionmentioning

confidence: 99%

A venom protein, Kazal‐type serine protease inhibitor, of ectoparasitoid Pachycrepoideus vindemiae inhibits the hemolymph melanization of host Drosophila melanogaster

Yang

Qiu

Fang

et al. 2020

Arch Insect Biochem Physiol

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Parasitic wasps inject various virulence factors into the host insects while laying eggs, among which the venom proteins, one of the key players in host insect/parasitoid relationships, act in host cellular and humoral immune regulation to ensure successful development of wasp progeny. Although the investigations into actions of venom proteins are relatively ample in larval parasitoids, their regulatory mechanisms have not been thoroughly understood in pupal parasitoids. Here, we identified a venom protein, Kazal-type serine protease inhibitor, in the pupal ectoparasitoid Pachycrepoideus vindemiae (PvKazal). Sequence analysis revealed that PvKazal is packed by a signal peptide and a highly conserved "Kazal" domain. Quantitative polymerase chain reaction analysis recorded a higher transcript level of PvKazal in the venom apparatus relative to that in the carcass, and the PvKazal messenger RNA level appeared to reach a peak on day 5 posteclosion. Recombinant PvKazal strongly inhibited the hemolymph melanization of host Drosophila melanogaster. Additionally, the heterologous expression of PvKazal in transgenic Drosophila reduced the crystal cell numbers and blocked the melanization of host pupal hemolymph. Our present work underlying the roles of PvKazal undoubtedly increases the understanding of venom-mediated hostparasitoid crosstalk.

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First report of the parasitoid Pachycrepoideus vindemmiae (Rondani, 1875) (Hymenoptera: Pteromalidae) parasitizing Synthesiomyia nudiseta (Van der Wulp, 1883) (Diptera: Muscidae)

Cited by 8 publications

References 9 publications

Identification and Comparative Analysis of Venom Proteins in a Pupal Ectoparasitoid, Pachycrepoideus vindemmiae

Identification and Comparative Analysis of Venom Proteins in a Pupal Ectoparasitoid, Pachycrepoideus vindemmiae

The Pupal Ectoparasitoid Pachycrepoideus vindemmiae Regulates Cellular and Humoral Immunity of Host Drosophila melanogaster

A venom protein, Kazal‐type serine protease inhibitor, of ectoparasitoid Pachycrepoideus vindemiae inhibits the hemolymph melanization of host Drosophila melanogaster

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