Influence of nitrogen sources on the enzymatic activity and grown by Lentinula edodes in biomass Eucalyptus benthamii

Abstract

Lignocellulose is the most abundant environmental component and a renewable organic resource in soil. There are some filamentous fungi which developed the ability to break down and use cellulose, hemicellulose and lignin as an energy source. The objective of this research was to analyze the effect of three nitrogen resources (ammonium sulfate, saltpetre, soybean) in the holocellulolitic activity of Lentinula edodes EF 50 using as substrate sawdust E. benthamii. An experimental design mixture was applied with repetition in the central point consisting of seven treatments (T) of equal concentrations of nitrogen in ammonium sulfate, potassium nitrate and soybean. The enzymatic activity of avicelase, carboxymetilcellulase, β-glucosidase, xylanases and manganese peroxidase was determined. The humidity, pH, water activity (a w ) and qualitative analysis of mycelial growth in 8 times of cultivation were evaluated. The results showed negative effect on enzyme production in treatments with maximum concentration of ammonium sulfate and potassium nitrate. The treatments with cooked soybean flour expressed higher enzymatic activities in times of 3, 6 and 9 days of culture, except in the activity of manganese peroxidase. The highest production was observed in the treatment with ammonium sulfate, and soybean (83.86 UI.L -1 ) at 20 days of cultivation.

Condiciones para el análisis de proteínas del micelio de Lentinula edodes obtenido por fermentación en estado líquido

“…Se utilizó micelio con un contenido de proteína total cruda de 289 + 9 mg de proteína / g micelio seco, lo cual corresponde a un porcentaje proteico de 28,9 + 0,9% [30] figura 2). Se ha observado que en el cultivo de macromicetos la presencia o ausencia de una fuente de nitrógeno influye en el contenido de proteínas del micelio [38], así como la naturaleza orgánica o inorgánica de la fuente [10]. Comparado con el contenido reportado en fructificaciones de L. edodes de Carneiro et al, con un valor de 12,76 % [52], o de Andrade et al, con valores de 20,7% [53], se observa que el medio de cultivo usado en el presente estudio presenta valores de contenido de proteína superiores.…”

“…L. edodes es una seta lignolítica [10] que ha sido usada como alimento o ingrediente de alimentos, debido a que se encuentra dentro del grupo de setas GRAS (por sus siglas en inglés "generally regarded as safe"). Por ello, la mayoría de las investigaciones realizadas sobre esta seta se han centrado en el cultivo tradicional o biotecnológico dirigido al aumento de su producción [11], siendo pocos los estudios que han profundizado en su perfil bioactivo.…”

Microbial manganese peroxidase: a ligninolytic enzyme and its ample opportunities in research

“…Ligninolytic enzyme (Lac, LiP, MnP) activity or production not only depends on the microbial species but also depends on culture conditions, carbon, and nitrogen sources and their concentration [61, 70,71]. Pedri et al [72] observed that maximum concentration of ammonium sulfate and potassium nitrate showed negative effects on enzyme activity, whereas maximum activity was observed in the presence of ammonium sulfate and soyabean. In culture broth, nitrogen source enhances microbial cell growth and also boosts the enzymes expressions [73].…”

Dehairing of animal hides and skins by alkaline proteases of Aspergillus oryzae for efficient processing to leather products in Tanzania

“…In this study, local isolates of A. oryzae (MG429773) from previous study (Zekeya et al, 2019) were used for production of alkaline protease using rice husks as medium. Use of rice husk supplemented with nitrogen source (Pedri et al, 2015) is reported to add value to over 4.1 million tons of rice husks produced annually in Tanzania with scarce utilization (Said et al, 2014). Furthermore, it replaces use of food stuff like sugar cane, corn wheat in solid state fermentation, which is threatening food security (Pensupa et al, 2013).…”