Acute inflammation and hematological response in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis

Dotta, Geovana; Ledic-Neto, J; Gonçalves, Eduardo Luiz Tavares; Brum, Aline; Maraschin, Marcelo; Martins, Maurício Laterça

doi:10.1590/1519-6984.02413

Cited by 12 publications

(7 citation statements)

References 19 publications

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“…Moreover, COX‐2 and PGDS1 are inducible enzymes involved in the synthesis of prostaglandins, the expression of which is limited to a few cell types, including acidophilic granulocytes (Gómez‐Abellán et al., 2015). The present results showed that the upregulated gene expression levels of the proinflammatory genes il‐1β and pgds1 in the HK in the carrageenin group at 1.5 hr and 3 hr, respectively, could be related to the release of mediators responsible for increased blood flow, vasodilation and vascular permeability in order to facilitate the migration of leucocytes from the systemic circulation to the locally inflamed area (Dotta et al., 2015; Iman et al., 2017; Martins et al., 2009; Ribas et al., 2016).…”

Section: Discussionmentioning

confidence: 84%

“…The available reports on the acute inflammation induced by λ-carrageenin demonstrated that the swim bladder of tilapia (Oreochromis niloticus) and pacu (Piaractus mesopotamicus) displayed vascular congestion, leucocyte infiltration and oedema 3 hr after administration. Furthermore, the severity of these conditions in the inflamed area peaked at 24 hr post-administration (Dotta et al, 2015;Martins et al, 2006;Matushima & Mariano, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…The available reports on the acute inflammation induced by λ‐carrageenin demonstrated that the swim bladder of tilapia ( Oreochromis niloticus ) and pacu ( Piaractus mesopotamicus ) displayed vascular congestion, leucocyte infiltration and oedema 3 hr after administration. Furthermore, the severity of these conditions in the inflamed area peaked at 24 hr post‐administration (Dotta et al., 2015; Martins et al., 2006; Matushima & Mariano, 1996). In a histopathological study wherein λ‐carrageenin was inoculated in the myotomal muscle on the dorsal side of plaice ( Pleuronectes platessa ), the polysaccharide remained extracellularly for days, producing a chronic inflammation until macrophages began to phagocytose it and store it into cytoplasmic vesicles, removing all the extracellular carrageenin at the end of the trial (Timur et al., 1977).…”

Section: Introductionmentioning

confidence: 99%

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Effects of subcutaneous injection of λ/κ‐carrageenin on the immune and liver antioxidant status of gilthead seabream (Sparusaurata)

Campos-Sánchez

Guardiola

Beltrán

et al. 2021

Journal of Fish Diseases

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This study investigated the acute inflammatory response induced by subcutaneous injection of carrageenin (1%) or phosphate‐buffered saline (control) in gilthead seabream (Sparus aurata). Skin mucus, serum, head kidney (HK) and liver were sampled at 1.5, 3 and 6 hr post‐injection (p.i.) to determine the immune and antioxidant status of this fish species. The skin mucus of the carrageenin group showed increased superoxide dismutase and peroxidase activities, lysozyme abundance, bactericidal activity against Vibrio anguillarum and Photobacterium damselae, and total immunoglobulins compared with those of the control group. However, the carrageenin‐injected fish sampled at 6 hr p.i. showed decreased protease activity in the skin mucus and peroxidase activity in the HK leucocytes compared with the control. Moreover, the carrageenin injection had no effects on the systemic immune system, but it reduced the liver catalase activities at both 3 and 6 hr in the carrageenin group relative to those in the control group. The expression levels of several proinflammatory and cell marker genes in the HK and liver were also determined. In the HK, the expression levels of interleukin‐1β and prostaglandin D synthase 1 were upregulated at 1.5 and 3 hr, respectively, in the carrageenin group compared with those in the control group. Contrarily, the expression of the NADPH oxidase subunit phox40 (an acidophilic granulocyte marker) in the carrageenin group at 6 hr was downregulated compared with that in the control group. These results suggested that subcutaneous injection of κ/λ‐carrageenin in gilthead seabream triggered an acute skin inflammation characterized by the rapid recruitment of acidophilic granulocytes and the release of humoral mediators into the skin mucus.

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of subcutaneous injection of λ/κ‐carrageenin on the immune and liver antioxidant status of gilthead seabream (Sparusaurata)

Campos-Sánchez

Guardiola

Beltrán

et al. 2021

Journal of Fish Diseases

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“…A chronic inflammatory lipid-depositing disease causes peripheral artery disease, coronary artery disease, and myocardial infarction (Gao and Liu 2017). A dysfunction of vessel endothelium can initiate plaques and lead to atherosclerosis (Dotta et al, 2015;Rajendran et al, 2013). Oxidative stress and inflammatory reactions are important risk factors for atherosclerosis progression (Steven et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Kirenol protects against oxidized low-density lipoprotein induced damages in endothelial cells

Zahra

2024

Braz. J. Biol.

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Kirenol (KNL) has recently been reported to have anti-inflammatory properties. Yet, little is known about the potential mechanisms of its anti-inflammatory properties. In HUVECs, we elucidated the anti-inflammatory mechanisms of kirenol. RT-PCR was used to test mRNA of pro-inflammatory mediators produced by Ox-LDL. The viability of cells was measured using MTT. Western blots analyzed protein levels. On Ox-LDL-stimulated HUVECs, KNL significantly inhibited the production of pro-inflammatory mediators such as NO, IL-1β, iNOS, TNF-α and IL-6. p38, ROS and Nrf2 expression were inhibited by KNL. Inhibition of p38, ROS, and KNL caused nuclear accumulation of Nrf2. KNL attenuated Ox-LDL-induced phosphorylation of ERK1/2 and p38, too. Based on our results, KNL inhibits NF-кB and MAPK signaling in HUVECs by activating Nrf2 signaling. There's a possibility that KNL could be developed into an anti-inflammatory drug.

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“…2014; Dotta et al, 2014Dotta et al, , 2015Farias et al, 2016) can affect the innate immune response of fish, what makes phagocytic assays an important tool for the evaluation of fish immunocompetence. However, few are the information regarding phagocytosis in Neotropical species (Jensch-Junior et al, 2006;Dias et al, 2011;Nunes et al, 2014;Farias et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

In vivo phagocytosis and hematology in Astyanax altiparanae, a potential model for surrogate technology

et al. 2020

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Abstract Although the potential of surrogate propagation technology for aquaculture and conservation of Neotropical fish, the poor understanding of the host immune system may results in rejection and destruction of the donor material. Thus, it is necessary to study and to develop methods to evaluate the effects of immunosuppressive drugs employment and to evaluate the immunocompatibility between donor and receptor. Thus, the present study aimed to optimize a methodology to assess in vivo phagocytosis in Astyanax altiparanae using Saccharomyces cerevisiae and to evaluate their hematological response resultant from the inflammatory induction. To this, S. cerevisiae were labeled with Congo red and injected in the coelomic cavity of A. altiparanae at the concentration of 2.5 x 106 cells mL-1. A PBS solution and a non-injected group were kept as control. Fish blood was sampled and the phagocytic capacity and index were determined at 1, 2, 3 and 6 h post-injection (hpi). The yeast injection successfully stimulated phagocytosis, with the best result for phagocytosis assessment after 2 hpi. Moreover, it was achieved a high traceability of phagocytized and non-phagocytized yeast under optic microscopy analysis due to the Congo red labeling. The hematological profile was similar to usually observed in early infections, indicating lymphocyte migration to inflammatory site and increase in number of circulating phagocytes due to natural response to inflammatory stimulus. In conclusion, our method was efficient to assess in vivo phagocytosis in A. altiparanae and will be an important tool to evaluate the efficacy of immunosuppressive drugs in this species. Additionally, these results may serve as support for further studies in fish immunocompetence, both in laboratory and in field conditions.

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Acute inflammation and hematological response in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis

Cited by 12 publications

References 19 publications

Effects of subcutaneous injection of λ/κ‐carrageenin on the immune and liver antioxidant status of gilthead seabream (Sparusaurata)

Effects of subcutaneous injection of λ/κ‐carrageenin on the immune and liver antioxidant status of gilthead seabream (Sparusaurata)

Kirenol protects against oxidized low-density lipoprotein induced damages in endothelial cells

In vivo phagocytosis and hematology in Astyanax altiparanae, a potential model for surrogate technology

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