2014
DOI: 10.1590/1415-52732014000500004
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Hematology and plasma biochemistry in rats fed with diets enriched with fatty fishes from Amazon region

Abstract: OBJECTIVE: Rats fed diets enriched with fatty fish from the Amazon region had Hematology and plasma biochemistry analyzed. METHODS: Forty Wistar rats were divided into four groups: control group fed a standard diet; mapará group fed a diet enriched with Hypophthalmus edentatus; matrinxã group fed a diet enriched with Brycon spp.; and tambaqui group fed a diet enriched with Colossoma macropomum. After thirty days the rats had an red blood count and plasma biochemistry. RESULTS: Hematocrit and hemoglobin levels … Show more

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“…RBCs count was carried out manually using a counting chamber. Hemoglobin concentrations in whole blood were measured using the cyanmethemoglobin method and mean cell hemoglobin (MCH) was determined according to Souza et al [ 16 ]. Evaluation of level of glycated hemoglobin (HbA1 c ) was based upon the generation of 5-hydroxymethylfurfural (5-HMF) from ketoamine after treatment with oxalic acid and its subsequent reaction with thiobarbituric acid (TBA) to form a coloured adduct.…”
Section: Methodsmentioning
confidence: 99%
“…RBCs count was carried out manually using a counting chamber. Hemoglobin concentrations in whole blood were measured using the cyanmethemoglobin method and mean cell hemoglobin (MCH) was determined according to Souza et al [ 16 ]. Evaluation of level of glycated hemoglobin (HbA1 c ) was based upon the generation of 5-hydroxymethylfurfural (5-HMF) from ketoamine after treatment with oxalic acid and its subsequent reaction with thiobarbituric acid (TBA) to form a coloured adduct.…”
Section: Methodsmentioning
confidence: 99%
“…The content of glycated hemoglobin (HbA1c) in blood was measured following the method described by Vitak et al [62], based on the generation of 5-hydroxymethylfurfural from ketoamine after treatment with oxalic acid and its subsequent reaction with thiobarbituric acid to form a colored adduct. Hemoglobin concentrations in the whole blood were determined using the cyanmethemoglobin method, whereas mean cell hemoglobin (MCH) was determined according to Souza et al [63]. The total number of erythrocytes and leukocytes in blood was calculated manually using a counting chamber.…”
Section: Methodsmentioning
confidence: 99%