lncRNA TUG1 promotes proliferation and differentiation of osteoblasts by regulating the miR-545-3p/CNR2 axis

Hao, Ruizheng; Wang, Bin; Wang, Hui; Huo, Yongxin; Yang, Lu

doi:10.1590/1414-431x20209798

Cited by 10 publications

(9 citation statements)

References 33 publications

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“… 25 To confirm whether miR‐34a is regulated by lncRNA, the lncRNAs that could interact with miR‐34a were predicted by StarBase database. Among the candidates, lncRNA TUG1, which is involved in regulating osteoblast proliferation and differentiation, 26 is an important potential target. The qRT‐PCR results demonstrated that lncRNA TUG1 levels were significantly increased in response to FSS in MC3T3‐E1 cells (Figure 6A ).…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, lncRNA TUG1 was up‐regulated in osteoblasts cultured under FSS, and lncRNA TUG1 could enhance osteoblast proliferation and inhibit osteoblast apoptosis. Hao et al 26 reported that lncRNA TUG1 sponged miR‐545‐3p to up‐regulate CNR2, facilitating osteoblast proliferation and differentiation in hFOB1.19 cells. Liu et al 41 discovered that lncRNA TUG1 accelerated osteoblast proliferation by Wnt pathway.…”

Section: Discussionmentioning

confidence: 99%

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Fluid shear stress regulates osteoblast proliferation and apoptosis via the lncRNA TUG1/miR‐34a/FGFR1 axis

Wang

et al. 2021

J Cellular Molecular Medi

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LncRNAs and microRNAs play critical roles in osteoblast differentiation and bone formation. However, their exact roles in osteoblasts under fluid shear stress (FSS) and the possible mechanisms remain unclear. The aim of this study was to explore whether and how miR‐34a regulates osteoblast proliferation and apoptosis under FSS. In this study, FSS down‐regulated miR‐34a levels of MC3T3‐E1 cells. MiR‐34a up‐regulation attenuated FSS‐induced promotion of proliferation and suppression of apoptosis. Luciferase reporter assay revealed that miR‐34a directly targeted FGFR1. Moreover, miR‐34a regulated osteoblast proliferation and apoptosis via FGFR1. Further, we validated that lncRNA TUG1 acted as a competing endogenous RNA (ceRNA) to interact with miR‐34a and up‐regulate FGFR1 protein expression. Furthermore, lncRNA TUG1 could promote proliferation and inhibit apoptosis. Taken together, our study revealed the key role of the lncRNA TUG1/miR‐34a/FGFR1 axis in FSS‐regulated osteoblast proliferation and apoptosis and may provide potential therapeutic targets for osteoporosis.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fluid shear stress regulates osteoblast proliferation and apoptosis via the lncRNA TUG1/miR‐34a/FGFR1 axis

Wang

et al. 2021

J Cellular Molecular Medi

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“…MiR-545-3p dysregulation has been reported in diverse cancers. Shi et al reported that miR-545-3p was downregulated in epithelial ovarian cancer (35); Hao et al showed that miR-545-3p inhibits the proliferation and differentiation of osteoblasts (36); and Zhong et al reported that miR-545-3p functions as a tumor suppressor in endometrial carcinoma (37). Furthermore, some studies indicate that miR-545-3p may be the target of circRNAs.…”

Section: Discussionmentioning

confidence: 99%

The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells

et al. 2021

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Glioma is the most common primary intracranial malignant tumour in adults. It has a high incidence and poses a serious threat to human health. Circular RNA is a hotspot of cancer research. In this study, we aimed to explore the role of circ_0001367 in gliomagenesis and the underlying mechanism. First, qRT-PCR was conducted, which showed that circ_0001367 level was downregulated in glioma tissues and cells. Next, gain-of-function and loss-of-function assays were performed, which indicated that circ_0001367 inhibited the proliferation, migration and invasion of glioma cells. Subsequent bioinformatics analysis, dual-luciferase reporter assays, RNA immunoprecipitation assays and cell function assays demonstrated that circ_0001367 inhibited the proliferation, migration and invasion of glioma cells by absorbing miR-545-3p and thereby regulating the expression of leucine zipper protein (LUZP1). Finally, an in vivo experiment was conducted, which demonstrated that circ_0001367 inhibited glioma growth in vivo by modulating miR-545-3p and LUZP1. Taken together, the results of this study demonstrate that the circ_0001367/miR-545-3p/LUZP1 axis may be a novel target for glioma therapy.

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“…Precious research has shown that miR-545-3p has pivotal roles in the regulating cellular behaviors, such as proliferation, differentiation and migration. 30 , 31 It has recently been identified as a cancer-related miRNA for several cancers. For example, Changjun et al declared that miR-545-3p was highly expressed in hepatocellular carcinoma tissues and cells, and miR-545-3p inhibition limited hepatocellular carcinoma cell proliferation and metastasis via regulating MT1M.…”

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA LINC01410 Suppresses Tumorigenesis and Enhances Radiosensitivity in Neuroblastoma Cells Through Regulating miR-545-3p/HK2 Axis

Mou¹,

Wang²,

Zhang³

et al. 2021

OTT

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Background: Abnormal expression of long noncoding RNAs (lncRNAs) was often involved in tumorigenesis and radiosensitivity of various cancers. The aim of this study was to explore the biological function and regulatory mechanism of lncRNA long intergenic non-protein coding RNA 1410 (LINC01410) in tumorigenesis and radiosensitivity of neuroblastoma (NB). Methods: The expression of LINC01410, microRNA-329-3p (miR-545-3p) and hexokinase 2 (HK2) was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Methylthiazolyldiphenyl tetrazolium bromide (MTT) assay, colony formation assay and transwell assay were utilized to detect cell viability, colony formation and cell invasion abilities. Glucose consumption or lactate production was measured by glucose assay kit or lactate assay kit, respectively. The interaction between miR-545-3p and LINC01410 or HK2 was predicted by starBase v2.0 and verified by dual-luciferase reporter, RNA Immunoprecipitation (RIP) and RNA pull-down assays. Western blot was used to measure the protein expression of HK2. The mice xenograft model was established to investigate the role of LINC01410 in vivo. Results: LINC01410 and HK2 were highly expressed while miR-545-3p was lowly expressed in NB tissues and cells. LINC01410 knockdown inhibited tumorigenesis by repressing cell proliferation and invasion, and increased the radiosensitivity via inhibiting colony formation rates and glycolysis. LINC01410 knockdown also suppressed tumor growth in vivo. Moreover, miR-545-3p could bind to LINC01410 and its downregulation reversed the effects of LINC01410 knockdown on tumorigenesis and radiosensitivity. Additionally, HK2 was a direct target of miR-545-3p and its overexpression attenuated the effects of miR-545-3p restoration on suppression of tumorigenesis and promotion of radiosensitivity. Besides, LINC01410 functioned as a molecular sponge of miR-545-3p to regulate HK2 expression. Conclusion: LINC01410 interference inhibited tumorigenesis and increased radiosensitivity via regulating miR-545-3p/HK2 axis, providing a novel therapeutic strategy for NB.

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lncRNA TUG1 promotes proliferation and differentiation of osteoblasts by regulating the miR-545-3p/CNR2 axis

Cited by 10 publications

References 33 publications

Fluid shear stress regulates osteoblast proliferation and apoptosis via the lncRNA TUG1/miR‐34a/FGFR1 axis

Fluid shear stress regulates osteoblast proliferation and apoptosis via the lncRNA TUG1/miR‐34a/FGFR1 axis

The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells

Long Noncoding RNA LINC01410 Suppresses Tumorigenesis and Enhances Radiosensitivity in Neuroblastoma Cells Through Regulating miR-545-3p/HK2 Axis

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