HtrA1 suppresses the growth of pancreatic cancer cells by modulating Notch-1 expression

Cheng, Hao; Zhu, Hao; Cao, Meng; Lu, Chunfeng; Bao, Shanshan; Pan, Yunlong

doi:10.1590/1414-431x20187718

Cited by 9 publications

(5 citation statements)

References 31 publications

(42 reference statements)

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“…HTRA1 has been reported to promote apoptosis in many types of tumor cells, such as esophageal squamous cell carcinoma 47 and pancreatic cancer cells. 48 Our study demonstrated that apoptosis was enhanced in GBM cells with depletion of HTRA1, and markers of apoptosis were also correspondingly regulated. These results indicated that HTRA1 inhibited apoptosis in human GBM cells.…”

Section: Discussionmentioning

confidence: 57%

HTRA1 promotes EMT through the HDAC6/Ac‐α‐tubulin pathway in human GBM cells

Zhao,

Wu,

Wang

et al. 2024

CNS Neurosci Ther

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BackgroundThe infiltrative nature of human gliomas renders complete surgical removal of tumors futile. Thus, illuminating mechanisms of their infiltrative properties may improve therapies and outcomes of glioma patients.MethodsComprehensive bioinformatic analyses of PRSS family were undertaken. Transfection of HTRA1 siRNAs was used to suppress HTRA1 expression. CCK‐8, EdU, and colony formation assay were employed to assess cell viability, and cell migration/invasion was detected by transwell, wound healing, and 3D tumor spheroid invasion assays. Immunoprecipitation was applied to study the mechanism that HTRA1 affected cell migration. In addition, in situ xenograft tumor model was employed to explore the role of HTRA1 in glioma growth in vivo.ResultsHTRA1 knockdown could lead to suppression of cell viability, migration and invasion, as well as increased apoptosis. Immunoprecipitation results indicates HTRA1 might facilitate combination between HDAC6 and α‐tubulin to enhance cell migration by decreasing α‐tubulin acetylation. Besides, HTRA1 knockdown inhibited the growth of xenografts derived from orthotopic implantation of GBM cells and prolonged the survival time of tumor‐bearing mice.ConclusionOur results indicate that HTRA1 promotes the proliferation and migration of GBM cells in vitro and in vivo, and thus may be a potential target for treatment in gliomas.

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Section: Discussionmentioning

confidence: 57%

HTRA1 promotes EMT through the HDAC6/Ac‐α‐tubulin pathway in human GBM cells

Zhao,

Wu,

Wang

et al. 2024

CNS Neurosci Ther

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“…ANXA3 (Annexin A3) is calcium-regulation dependent, involved in signal transduction, acts as a maintenance of cellular activity, and promotes lung carcinogenesis [33,34]. HTRA1 (HtrA serine peptidase 1) downregulation on infection and in ammation regulates proteases, involved in oxidative stress, cellular carcinogenesis and other stress conditions [35][36][37]. These epigenetic modi cation related proteins were signi cantly different after TET1 overexpression, and were involved in affecting the cell viability, gene transcription and translation activities and important intracellular biological processes of SSCs.…”

Section: Discussionmentioning

confidence: 99%

Quantitative proteomic analysis based on tandem mass tagging (TMT) reveals key proteins related to DNA hydroxymethylation enzyme TET1 for spermatogonia self-renewal

Liu

Wang

Tao

et al. 2022

Preprint

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Background Abnormal spermatogonia (SSCs) can cause spermatogenic disorders such as weak spermatozoa, oligospermia, and azoospermia. DNA hydroxymethylase TET1 hydroxylates the methylation sites of specific genes, enabling the process of DNA demethylation and regulating gene expression. However, the key differential genes affected by the specific action of TET1 and the mechanism of interaction between the differential genes are not clear. Result In this study, we applied quantitative proteomics techniques based on Tandem Mass Tags (TMT) to screen the 24h differentially expressed proteins in the TET1 overexpression group (MYC-TET1) and the control group (MYC) to provide a basis for studies such as the regulation of TET1-mediated epigenetic modifications on SSCs. By TMT technique, we identified 5891 proteins, of which 337 were significantly differentially expressed, 76 were up-regulated and 261 were down-regulated. Gene Ontology (GO) enrichment analysis revealed that proteins with significant differential expression such as RARG, RN114, DJC30, and ABHD2 were associated with functions such as sperm-egg recognition, sperm-egg fusion, sperm ejaculation, spermatogenesis and development, and embryonic development. changes in proteins such as GHR, CCNT1, HTRA1, and ANXA3 affected cell viability, gene transcription and translation activities, and important intracellular biological processes in SSCs. intracellular biological processes. Conclusions In this study, we obtained differential protein profiles by overexpressing TET1 in SSCs and subsequently by TMT protein sequencing technology, combined with Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway analysis, GO enrichment analysis and protein interaction network map to jointly analyze the epistatic regulatory role of TET1 on SSCs, which provides a scientific basis for further study of spermatogenesis and contributes to the understanding of male reproductive system diseases.

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“…HtrA1 belongs to the serine protease family, and its deficient expression has been reported in several human cancers, such as pancreatic cancer [20], breast cancer [21], and hepatocellular carcinoma [22]. HtrA1 can suppress malignant development and progression of cancer [23], and it also has been reported to be involved in chemotherapy resistance.…”

Section: Discussionmentioning

confidence: 99%

Effect of HtrA1 Polymorphism on Sensitivity to Chemotherapy in Patients with Colon Cancer

Yao

2020

Med Sci Monit

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Departmental sources Background:This study was performed to estimate the genetic effects of HtrA1 polymorphisms rs1049331 and rs11200638 on treatment response in stage III colon cancer patients receiving 5-FU-based chemotherapy. Material/Methods:A total of 105 stage III colon cancer patients who received postoperative 5-FU based adjuvant chemotherapy were included in our study. Chemotherapy was performed in 3 cycles for the patients. HtrA1 rs1049331 and rs11200638 polymorphisms were genotyped via polymerase chain reaction with sequencing method. The treatment response was estimated according to the RECIST guidelines. Results:The response rate of the eligible patients was 53.33%. For rs1049331, the presences of TT genotype and T allele indicted reduced chemotherapy sensitivity (adjusted TT: OR=1.736, 95%CI: 1.001-3.011, P=0.049; T: OR=1.801, 95%CI: 1.054-2.932, P=0.039). The rs11200638 polymorphism had no significant association with chemotherapy sensitivity in the study population (P>0.05 for all). Conclusions:HtrA1 rs1049331 polymorphism, but not rs11200638 polymorphism, can influence individual sensitivity to 5-FU-based treatment in stage III colon cancer patients.

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HtrA1 suppresses the growth of pancreatic cancer cells by modulating Notch-1 expression

Cited by 9 publications

References 31 publications

HTRA1 promotes EMT through the HDAC6/Ac‐α‐tubulin pathway in human GBM cells

HTRA1 promotes EMT through the HDAC6/Ac‐α‐tubulin pathway in human GBM cells

Quantitative proteomic analysis based on tandem mass tagging (TMT) reveals key proteins related to DNA hydroxymethylation enzyme TET1 for spermatogonia self-renewal

Effect of HtrA1 Polymorphism on Sensitivity to Chemotherapy in Patients with Colon Cancer

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