Serum microRNA-30c levels are correlated with disease progression in Xinjiang Uygur patients with chronic hepatitis B

Zhang, J; Ma, Juncheng; Wang, H; Guo, Linlin; Li, J

doi:10.1590/1414-431x20176050

Cited by 3 publications

(4 citation statements)

References 25 publications

(29 reference statements)

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“…In accordance with these studies, in the present study RRM2 was predicted to be induced by HBV to promote the development of HCC and the high expression of RRM2 may serve as a poor prognostic factor. Previously, overexpression of miR-30c was reported to significantly inhibit cell proliferation and delay G1/S phase transition in hepatoma cells by blocking the HBV replication and the expression of HBV pgRNA, capsid-associated virus DNA and Hbx, indicating its tumor suppressive roles (36). As anticipated, miR-30c was also revealed to be downregulated in HBV-HCC samples.…”

Section: Discussionmentioning

confidence: 99%

SSTR5‑AS1 functions as a ceRNA to regulate CA2 by sponging miR‑15b‑5p for the development and prognosis of HBV‑related hepatocellular carcinoma

Zhang

Wen

et al. 2019

Mol Med Report

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long non-coding rnas (lncrnas) have been implicated in the development and progression of cancer. However, the mechanisms of lncrnas in hepatitis B virus (HBV) infection-induced hepatocellular carcinoma (Hcc) remain unclear. The study aimed to reveal the roles of lncrnas for HBV-Hcc based on the hypothesis of competing endogenous rna (cerna). The lncrna (GSe27462), mirna (GSe76903) and mrna (GSe121248) expression profiles were collected from the Gene Expression Omnibus database. differentially expressed lncrnas (dels), genes (deGs) and mirnas (deMs) were identified using the liMMa or edger package, respectively. The cerna network was constructed based on interaction pairs between mirnas and mrnas/lncrnas. The functions of deGs in the cerna network were predicted using the daVid database, which was overlapped with the known Hcc pathways of comparative Toxicogenomics database (cTd) to construct the Hcc-related cerna network. The prognosis values [overall survival, (oS); recurrence-free survival (rFS)] of genes were validated using the cancer Genome atlas (TcGa) data with cox regression analysis. The present study screened 38 dels, 127 deMs and 721 deGs. a cerna network was constructed among 17 dels, 12 deMs and 173 deGs, including the FaM138B-hsa-mir-30c-ccne2/rrM2 and SSTr5-aS1-hsa-mir-15b-5p-ca2 cerna axes. Function enrichment analysis revealed the genes in the cerna network that participated in the p53 signaling pathway [cyclin e2 (ccne2), ribonucleotide reductase M2 subunit (rrM2)] and nitrogen metabolism [carbonic anhydrase 2 (ca2)], which were also included in the pathways of the cTd. univariate cox regression analysis revealed that six rnas (2 dels: FaM138B, SSTr5-aS1; 2 deMs: hsa-mir-149, hsa-mir-7; 2 deGs: ccne2, rrM2) were significantly associated with oS; while seven rnas (1 del: linc00284; 3 deMs: hsa-mir-7, hsa-mir-15b, hsa-mir-30c-2; and 3 deGs: rrM2, ccne2, ca2) were significantly associated with rFS. in conclusion, FaM138B-hsa-mir-30c-ccne2/rrM2 and the SSTr5-aS1-hsa-mir-15b-5p-ca2 cerna axes may be important mechanisms for HBV-related Hcc.

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Section: Discussionmentioning

confidence: 99%

SSTR5‑AS1 functions as a ceRNA to regulate CA2 by sponging miR‑15b‑5p for the development and prognosis of HBV‑related hepatocellular carcinoma

Zhang

Wen

et al. 2019

Mol Med Report

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“…Overexpression of miR‐30c could also significantly inhibited HBV replication and the expressions of HBV pgRNA, capsid‐associated virus DNA and Hbx in hepatoma cells. However, the definite mechanism was not further explored . Additionally, a recent study demonstrated that HBV infection could up‐regulate miR‐17 to 92 cluster through c‐Myc.…”

Section: Discussionmentioning

confidence: 99%

“…However, the definite mechanism was not further explored. 30 Additionally, a recent study 31 Finally, the following limitations merit consideration. Firstly, due to OBI patients have the characteristics of apparent health, P-value threshold used as filtering criteria in this study was < .1, which is different from that commonly accepted.…”

Section: Discussionmentioning

confidence: 99%

Circulating miRNA expression profile and bioinformatics analysis in patients with occult hepatitis B virus infection

Hao

Wang

Xia

et al. 2019

Journal of Medical Virology

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Emerging suggest that microRNAs (miRNAs) play vital roles in the occurrence and development of hepatitis B virus (HBV) infectious disease. However, miRNAs in occult hepatitis B virus infection (OBI), a special stage of HBV infection, remain largely unknown. Herein, we conducted this study to identify differentially expressed miRNAs and then to investigate the potential roles of these miRNAs in OBI. Plasma miRNA expression profiles of three OBI patients and three healthy controls were analyzed with high through‐put miRNA sequencing technology. Altered expression of miRNAs was further confirmed with reverse transcription quantitative polymerase chain reaction (qRT‐PCR). Finally, bioinformatics analysis was conducted to investigate the involved pathways and target genes for these differentially expressed miRNAs. Totally, 32 differentially expressed miRNAs were identified between OBI and healthy controls by miRNA sequencing (fold change ≥ 1.5, P < .1, and counts per million reads ≥ 1), including 16 downregulated and 16 upregulated miRNAs. Differential expression of hsa‐miR‐486‐5p, ‐25‐3p, and ‐92a‐3p and ‐1‐3p was further validated by qRT‐PCR analysis, which was consistent with miRNA sequencing analysis. Moreover, these four miRNAs might distinguish OBI from HCs efficiently. Bioinformatics analyses indicated that the differentially expressed miRNAs were primarily involved in various biological processes related to gene expression and transcription, cell development and metabolism, protein modification and kinase activity regulation, as well as multiple signaling pathways such as PI3K/Akt signaling pathway. This study provided a global view of miRNA expression in plasma from OBI patients. These differentially expressed miRNAs might play important roles in the development of OBI, which provided intriguing insights into the screening and molecular mechanism of OBI.

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“…Down-regulation of miR-30c may result in the progression of chronic HBV via the promotion of HBV replication and cell proliferation [45]. The ability of the full-length HBx protein and its truncated forms to act on the cell cycle of regulatory proteins is one of the main elements of pathogenesis in the development of HCC [46].…”

Section: Introductionmentioning

confidence: 99%

The First Yarrowia lipolytica Yeast Models Expressing Hepatitis B Virus X Protein: Changes in Mitochondrial Morphology and Functions

et al. 2022

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Chronic hepatitis B virus infection is the dominant cause of hepatocellular carcinoma, the main cause of cancer death. HBx protein, a multifunctional protein, is essential for pathogenesis development; however, the underlying mechanisms are not fully understood. The complexity of the system itself, and the intricate interplay of many factors make it difficult to advance in understanding the mechanisms underlying these processes. The most obvious solution is to use simpler systems by reducing the number of interacting factors. Yeast cells are particularly suitable for studying the relationships between oxidative stress, mitochondrial dynamics (mitochondrial fusion and fragmentation), and mitochondrial dysfunction involved in HBx-mediated pathogenesis. For the first time, genetically modified yeast, Y. lipolytica, was created, expressing the hepatitis B virus core protein HBx, as well as a variant fused with eGFP at the C-end. It was found that cells expressing HBx experienced stronger oxidative stress than the control cells. Oxidative stress was alleviated by preincubation with the mitochondria-targeted antioxidant SkQThy. Consistent with these data, in contrast to the control cells (pZ-0) containing numerous mitochondrial forming a mitochondrial reticulum, in cells expressing HBx protein, mitochondria were fragmented, and preincubation with SkQThy partially restored the mitochondrial reticulum. Expression of HBx had a significant influence on the bioenergetic function of mitochondria, making them loosely coupled with decreased respiratory rate and reduced ATP formation. In sum, the first highly promising yeast model for studying the impact of HBx on bioenergy, redox-state, and dynamics of mitochondria in the cell and cross-talk between these parameters was offered. This fairly simple model can be used as a platform for rapid screening of potential therapeutic agents, mitigating the harmful effects of HBx.

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Serum microRNA-30c levels are correlated with disease progression in Xinjiang Uygur patients with chronic hepatitis B

Cited by 3 publications

References 25 publications

SSTR5‑AS1 functions as a ceRNA to regulate CA2 by sponging miR‑15b‑5p for the development and prognosis of HBV‑related hepatocellular carcinoma

SSTR5‑AS1 functions as a ceRNA to regulate CA2 by sponging miR‑15b‑5p for the development and prognosis of HBV‑related hepatocellular carcinoma

Circulating miRNA expression profile and bioinformatics analysis in patients with occult hepatitis B virus infection

The First Yarrowia lipolytica Yeast Models Expressing Hepatitis B Virus X Protein: Changes in Mitochondrial Morphology and Functions

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