SSTR5‑AS1 functions as a ceRNA to regulate CA2 by sponging miR‑15b‑5p for the development and prognosis of HBV‑related hepatocellular carcinoma

Xu, Jing; Zhang, Jing; Wen, Jie; Wang, Yue

doi:10.3892/mmr.2019.10736

Cited by 29 publications

(27 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…sysu.edu.cn/) [19], respectively. Only the DEL-DEM and DEM-DEG interactors that had an opposite expression trend were used to construct the ceRNA network [20]. e interactions between DEGs were predicted by mapping the genes to the Search Tool for the Retrieval of Interacting Genes (STRING; version, 10.0; http://string-db.org/) database [21].…”

Section: Function Prediction Of the Prognostic Rnasmentioning

confidence: 99%

Recurrence-Associated Multi-RNA Signature to Predict Disease-Free Survival for Ovarian Cancer Patients

Chen

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

Ovarian cancer (OvCa) is an intractable gynecological malignancy due to the high recurrence rate. Several molecular biomarkers have been previously screened for early identifying patients with a high recurrence risk and poor prognosis. However, all the known studies focused on a single type of RNAs, not integrating various types. This study was to construct a new multi-RNA-based model to predict the recurrence and prognosis for OvCa patients by using the messenger RNA (mRNA, including long noncoding RNA (lncRNA)) and microRNA (miRNA) sequencing data of The Cancer Genome Atlas database. After univariate Cox regression and least absolute shrinkage and selection operator analyses, a multi-RNA-based signature (2 miRNAs: hsa-miR-508, hsa-miR-506; 1 lncRNA: TM4SF1-AS1; 11 mRNAs: MAGI3, SLAMF7, GLI2, PDK1, ARID3A, PLEKHG4B, TNFAIP8L3, C1QTNF3, NDUFAF1, CH25H, TMEM129) was generated and used to establish a risk score model. The high- and low-risk patients classified by the median risk score exhibited significantly different recurrence risks (89% versus 61%, p<0.001) and survival time (the area under the receiver operating characteristic curve (AUC) = 0.901 for 5-year disease-free survival (DFS)). This risk model was independent of other clinical features and superior to pathologic staging for DFS prediction (AUC, 0.906 versus 0.524; C-index, 0.633 versus 0.510). Furthermore, some new interaction axes were revealed to explain the possible functions of these RNAs (competing endogenous RNA: TM4SF1-AS1-miR-186-STEAP2, LINC00536-miR-508-STEAP2, LINC00475-miR-506-TMEM129; coexpression: LINC00598-PLEKHG4B). In conclusion, this multi-RNA-based risk model may be clinically useful to stratify OvCa patients with different recurrence risks and survival outcomes and included RNAs may be potential therapeutic targets.

show abstract

Section: Function Prediction Of the Prognostic Rnasmentioning

confidence: 99%

Recurrence-Associated Multi-RNA Signature to Predict Disease-Free Survival for Ovarian Cancer Patients

Chen

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Carbonic anhydrase 2 (CA2) encodes one of isozymes of carbonic anhydrase which catalyzes reversible hydration of carbon dioxide and plays a pivotal role in tissue pH homeostasis [20]. The expression of CA2 remains controversial in different cancers, it was reported to be upregulated in urinary bladder cancers [21], and CA2 autoantibody titers in gastric cancer patients were found higher compared to healthy subjects [22], while in esophageal adenocarcinoma, its expression was downregulated [23].…”

Section: Discussionmentioning

confidence: 99%

Identification of novel biomarkers affecting the metastasis of colorectal cancer through bioinformatics analysis and validation through qRT-PCR

et al. 2020

View full text Add to dashboard Cite

Background: Tumor progression and distant metastasis are the main causes of deaths in colorectal cancer (CRC) patients, and the molecular mechanisms in CRC metastasis have not been completely discovered. Methods: We identified differentially expressed genes (DEGs) and lncRNAs (DELs) of CRC from The Cancer Genome Atlas (TCGA) database. Then we conducted the weighted gene co-expression network analysis (WGCNA) to investigate co-expression modules related with CRC metastasis. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, DEG-DEL co-expression network and survival analyses of significant modules were also conducted. Finally, the expressions of selected biomarkers were validated in cell lines by quantitative real-time PCR (qRT-PCR). Results: 2032 DEGs and 487 DELs were involved the construction of WGCNA network, and greenyellow, turquoise and brown module were identified to have more significant correlation with CRC metastasis. GO and KEGG pathway analysis of these three modules have proven that the functions of DEGs were closely involved in many important processes in cancer pathogenesis. Through the DEG-DEL co-expression network, 12 DEGs and 2 DELs were considered as hub nodes. Besides, survival analysis showed that 30 DEGs were associated with the overall survival of CRC. Then 10 candidate biomarkers were chosen for validation and the expression of CA2, CHP2, SULT1B1, MOGAT2 and C1orf115 were significantly decreased in CRC cell lines when compared to normal human colonic epithelial cells, which were consistent with the results of differential expression analysis. Especially, low expression of SULT1B1, MOGAT2 and C1orf115 were closely correlated with poorer survival of CRC. Conclusion: This study identified 5 genes as new biomarkers affecting the metastasis of CRC. Besides, SULT1B1, MOGAT2 and C1orf115 might be implicated in the prognosis of CRC patients.

show abstract

“…For instance, SSTR5-AS1 functions as a ceRNA to regulate CA2 expression by interacting with miR-15b-5p in development of Hepatitis B virus (HBV)-related hepatocellular carcinoma. 10 Upregulation of lncRNA NNT-AS1 facilitates epithelialmesenchymal transition (EMT) of cholangiocarcinoma through miR-142-5p/HMGA2 axis. 11 LncRNA HCP5 regulates gemcitabine resistance for pancreatic cancer cells via sponging miR-214-3p and modulating HDGF.…”

Section: Introductionmentioning

confidence: 99%

“…ceRNA pattern is a typical posttranscriptional mechanism in which lncRNA competes with messenger (mRNA) to bind microRNA (miRNA), thus relieving the inhibition of miRNA on mRNA. For instance, SSTR5‐AS1 functions as a ceRNA to regulate CA2 expression by interacting with miR‐15b‐5p in development of Hepatitis B virus (HBV)‐related hepatocellular carcinoma 10 . Upregulation of lncRNA NNT‐AS1 facilitates epithelial‐mesenchymal transition (EMT) of cholangiocarcinoma through miR‐142‐5p/HMGA2 axis 11 .…”

Section: Introductionmentioning

confidence: 99%

PCAT6 mediates cellular biological functions in gastrointestinal stromal tumor via upregulation of PRDX5 and activation of Wnt pathway

Bai¹,

Zhang

Fang

et al. 2020

Molecular Carcinogenesis

View full text Add to dashboard Cite

Gastrointestinal stromal tumor (GIST) is a common mesenchymal tumor in the gastrointestinal tract. Prostate cancer associated transcript 6 (PCAT6) is a long noncoding RNA (lncRNA) and plays a pivotal role in tumor formation. Present study was designed to explore the function of PCAT6 in GIST. Ki67 staining, colony formation and trypan blue staining assays revealed that PCAT6 boosted GIST cell proliferation but inhibited cell apoptosis. Also, sphere formation assay and Western blot uncovered the promoting role of PCAT6 in GIST stemness. Then, we identified that PCAT6 could activate Wnt/β-catenin pathway. And the tumor facilitator role of Wnt/β-catenin pathway was validated in the rescue assays. Next, miR-143-3p was identified as the downstream microRNA of PCAT6. Moreover, miR-143-3p itself served as a tumor suppressor in GIST. Subsequently, peroxiredoxin 5 (PRDX5) was verified as the target of miR-143-3p. PCAT6 promoted GIST cell proliferation and stemness via sponging miR-143-3p to upregulate PRDX5. In a word, PCAT6 promoted GIST cell proliferation and stemness but inhibited cell apoptosis via competing endogenous RNA pattern and activation of Wnt pathway, which might contribute to GIST treatment. K E Y W O R D S ceRNA, gastrointestinal stromal tumor, PCAT6, PRDX5, Wnt/β-catenin

show abstract

SSTR5‑AS1 functions as a ceRNA to regulate CA2 by sponging miR‑15b‑5p for the development and prognosis of HBV‑related hepatocellular carcinoma

Cited by 29 publications

References 49 publications

Recurrence-Associated Multi-RNA Signature to Predict Disease-Free Survival for Ovarian Cancer Patients

Recurrence-Associated Multi-RNA Signature to Predict Disease-Free Survival for Ovarian Cancer Patients

Identification of novel biomarkers affecting the metastasis of colorectal cancer through bioinformatics analysis and validation through qRT-PCR

PCAT6 mediates cellular biological functions in gastrointestinal stromal tumor via upregulation of PRDX5 and activation of Wnt pathway

Contact Info

Product

Resources

About