Enhanced production and organic solvent stability of a protease fromBrevibacillus laterosporus strain PAP04

Abstract

A bacterial strain (PAP04) isolated from cattle farm soil was shown to produce an extracellular, solvent-stable protease. Sequence analysis using 16S rRNA showed that this strain was highly homologous (99%) to Brevibacillus laterosporus. Growth conditions that optimize protease production in this strain were determined as maltose (carbon source), skim milk (nitrogen source), pH 7.0, 40°C temperature, and 48 h incubation. Overall, conditions were optimized to yield a 5.91-fold higher production of protease compared to standard conditions. Furthermore, the stability of the enzyme in organic solvents was assessed by incubation for 2 weeks in solutions containing 50% concentration of various organic solvents. The enzyme retained activity in all tested solvents except ethanol; however, the protease activity was stimulated in benzene (74%) followed by acetone (63%) and chloroform (54.8%). In addition, the plate assay and zymography results also confirmed the stability of the PAP04 protease in various organic solvents. The organic solvent stability of this protease at high (50%) concentrations of solvents makes it an alternative catalyst for peptide synthesis in non-aqueous media.

Thermoflavimicrobium dichotomicum as a novel thermoalkaliphile for production of environmental and industrial enzymes

“…Also, it goes up for solving problems by producing of producing nontraditional materials as antimicrobial agents [4][5][6], nanobiosynthesized metals like silver, copper and zinc [7,8] and biotechnological agents as bioactive materials [9][10][11][12]. Extremophile microorganisms have various adaptation mechanisms enabling them to thrive and produce secondary metabolites under harsh conditions [13][14][15]. Extremophiles are ≤ H y [6][7][8][9][10][11][12][13][14][15][16][17][18].…”

“…Extremophile microorganisms have various adaptation mechanisms enabling them to thrive and produce secondary metabolites under harsh conditions [13][14][15]. Extremophiles are ≤ H y [6][7][8][9][10][11][12][13][14][15][16][17][18]. Extremophiles isolates from different habitats include soil, compost, manure [19], geothermal areas [20], sewage sludge or municipal solid waste [21], oil wells [22] and thermally treated foods [23].…”

Identification of Protease-Producing Halophilic Bacteria Isolated from Salt-Pond Soil

“…Skim milk in the media, for instance, was the most effective nitrogen source to increase protease production. In addition, there were several other sources of nitrogen, such as casein and gelatin (Anbu, 2016). Several studies that identified proteolytic bacteria from several sources aimed at the application of these microbes in scientific development activities and biotechnology (Ebrahempour and Ashraf, 2015).…”

Production of Proline and Protease With Different Organic Wastes in Bacteria

“…Microbial proteases act like virulence factors and are known to damage the defense proteins of the host organism. Commonly used microorganisms for protease production include Streptococcus sp., Bacillus sp., Streptobacillus sp., P. aeruginosa, and E. coli [17][18][19][20].…”