Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts

Zhang, L.; Ding, Yasuo; Rao, Guo-zhou; Miao, Dongmei

doi:10.1590/1414-431x20154324

Cited by 17 publications

(9 citation statements)

References 27 publications

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“…Periodontal ligament (PDL) fibroblasts may participate in periodontal inflammation by releasing cytokines and chemokines (Garlet et al 2008; Jönsson et al 2009; Zhang et al 2016). PDL cells are fibroblast-like cells but also exhibit some osteoblastic features.…”

Section: Introductionmentioning

confidence: 99%

Diabetes Activates Periodontal Ligament Fibroblasts via NF-κB In Vivo

et al. 2018

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Diabetes mellitus increases periodontitis and pathogenicity of the oral microbiome. To further understand mechanisms through which diabetes affects periodontitis, we examined its impact on periodontal ligament fibroblasts in vivo and in vitro. Periodontitis was induced by inoculation of Porphyromonas gingivalis and Fusobacterium nucleatum in normoglycemic and diabetic mice. Diabetes, induced by multiple low-dose injections of streptozotocin increased osteoclast numbers and recruitment of neutrophils to the periodontal ligament, which could be accounted for by increased CXC motif chemokine 2 (CXCL2) and receptor activator of nuclear factor kappa B ligand (RANKL) expression by these cells. Diabetes also stimulated a significant increase in nuclear factor kappa B (NF-κB) expression and activation in periodontal ligament (PDL) fibroblasts. Surprisingly, we found that PDL fibroblasts express a 2.3-kb regulatory unit of Col1α1 (collagen type 1, alpha 1) promoter typical of osteoblasts. Diabetes-enhanced CXCL2 and RANKL expression in PDL fibroblasts was rescued in transgenic mice with lineage-specific NF-κB inhibition controlled by this regulatory element. In vitro, high glucose increased NF-κB transcriptional activity, NF-κB nuclear localization, and RANKL expression in PDL fibroblasts, which was reduced by NF-κB inhibition. Thus, diabetes induces changes in PDL fibroblast gene expression that can enhance neutrophil recruitment and bone resorption, which may be explained by high glucose-induced NF-κB activation. Furthermore, PDL fibroblasts express a regulatory element in vivo that is typical of committed osteoblasts.

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Section: Introductionmentioning

confidence: 99%

Diabetes Activates Periodontal Ligament Fibroblasts via NF-κB In Vivo

et al. 2018

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“…This reduction presumably related to the increasing level of CRP, IL-6, fibrinogen, glycemic status, inflammation, and insulin resistance. 12,30,31 HBOT increased the OPG expression considerably compared with that present under healthy and diabetic periodontis conditions. OPG is produced by osteoblasts and other cell types, including peripheral blood lymphocytes.…”

Section: Discussionmentioning

confidence: 83%

“…This reduction presumably related to the increasing level of CRP, IL-6, fibrinogen, glycemic status, inflammation, and insulin resistance. 12 30 31 …”

Section: Discussionmentioning

confidence: 99%

The Impact of Hyperbaric Oxygen Therapy on Serum C-Reactive Protein Levels, Osteoprotegerin Expression, and Osteoclast Numbers in Induced-Periodontitis Diabetic Rats

2020

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Objectives This study aimed to examine the impact of hyperbaric oxygen therapy (HBOT) on serum C-reactive protein (CRP) levels, osteoclast numbers, and osteoprotegerin (OPG) expression in periodontitis-induced diabetic rats Materials and Methods This study constituted an in vivo laboratory-based experiment incorporating a posttest only control group design. Thirty male Wistar rats were divided into three groups of research subjects: a healthy group (K0), periodontitis-induced diabetic group (K1), and periodontitis-induced diabetic group treated with HBOT for 7 days (K2). After treatment, the subjects were sacrificed to determine the level of serum CRP by the ELISA method. Immunohistochemical analysis was conducted to check the level of OPG expression, while a histological analysis was undertaken to quantify the number of osteoclasts. Statistical Analysis The data was analyzed using a one-way ANOVA and Least Significant Difference (LSD) test on which a result of p < 0.05 was considered statistically significant. Results HBOT appreciably decreased serum CRP levels, significantly enhancing OPG expression in periodontitis-induced diabetic (p < 0.05) and decreasing the number of osteoclasts in -periodontitis-induced diabetic (p > 0.05). Conclusion HBOT reduced the serum CRP level, increased OPG expression, and decreased osteoclast numbers in periodontitis-induced diabetic rats.

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“…The high concentration of IL-10 in the inflammatory phase will largely inhibit the recruitment of macrophages and subsequently affect the amount of macrophages secreted cytokines [ 27 ]. While the inhibitory effect of IL-10 on fibroblast proliferation in proliferative phase depends on a higher effective concentration [ 8 , 28 , 29 ]. Therefore, how to keep IL-10 at a relative low level during the inflammatory phase and maintain it at a higher level during the proliferative phase is a critical issue.…”

Section: Resultsmentioning

confidence: 99%

Programmable immune activating electrospun fibers for skin regeneration

Chen

Zhang

et al. 2021

Bioactive Materials

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Immune cells play a crucial regulatory role in inflammatory phase and proliferative phase during skin healing. How to programmatically activate sequential immune responses is the key for scarless skin regeneration. In this study, an “Inner-Outer” IL-10-loaded electrospun fiber with cascade release behavior was constructed. During the inflammatory phase, the electrospun fiber released a lower concentration of IL-10 within the wound, inhibiting excessive recruitment of inflammatory cells and polarizing macrophages into anti-inflammatory phenotype “M2c” to suppress excessive inflammation response. During the proliferative phase, a higher concentration of IL-10 released by the fiber and the anti-fibrotic cytokines secreted by polarized “M2c” directly acted on dermal fibroblasts to simultaneously inhibit extracellular matrix overdeposition and promote fibroblast migration. The “Inner-Outer” IL-10-loaded electrospun fiber programmatically activated the sequential immune responses during wound healing and led to scarless skin regeneration, which is a promising immunomodulatory biomaterial with great potential for promoting complete tissue regeneration.

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Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts

Cited by 17 publications

References 27 publications

Diabetes Activates Periodontal Ligament Fibroblasts via NF-κB In Vivo

Diabetes Activates Periodontal Ligament Fibroblasts via NF-κB In Vivo

The Impact of Hyperbaric Oxygen Therapy on Serum C-Reactive Protein Levels, Osteoprotegerin Expression, and Osteoclast Numbers in Induced-Periodontitis Diabetic Rats

Programmable immune activating electrospun fibers for skin regeneration

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