Gene expression of estrogen and oxytocin receptors in the uterus of pregnant and parturient bitches

Veiga, Giselle Almeida Lima da; Milazzotto, Marcella Pécora; Nichi, Marcílio; Lúcio, Cristina de Fátima; Silva, L. C. G; Angrimani, Daniel de Souza Ramos; Vannucchi, Camila Infantosi

doi:10.1590/1414-431x20143969

Cited by 17 publications

(18 citation statements)

References 28 publications

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“…Until now, the mechanism that triggers such changes in dogs is unknown, as longitudinal studies along the periods of pregnancy and parturition are scarce. In previous works, we have observed that both endometrial ERα mRNA and OTR mRNA remained unchanged until late pregnancy, but the expression of OTR mRNA in canine uterine tissues (endometrium and myometrium) varied over time (Veiga et al, 2015). Additionally, we have shown that the canine CL does not express ERα mRNA during pregnancy, whereas a decreased profile of OTR mRNA expression occurs (Veiga et al, 2014).…”

Section: Introductionmentioning

confidence: 65%

“…Sample tubes without placenta tissue were used for negative controls. No positive controls were adopted, however primers were previously standardized and simultaneously employed for amplification in other canine tissue samples (endometrium, myometrium and corpus luteum), which revealed positive amplifications (Veiga et al, 2014;2015). All reactions were performed in a total volume of 25 µL and heated to 50°C for 2 min and then 95°C for 10 min followed by 45 cycles comprising denaturation at 95°C for 15 sec and then annealing for 60 sec at the following temperatures: 61°C for RPS5, 60°C for 18S and 59°C for ERα and OTR.…”

Section: Real-time Pcr Amplificationmentioning

confidence: 99%

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Correlation of Oxytocin (OTR) and Estrogen Receptor (ER) mRNA in the Canine Placenta with the Detected Circulating Levels of Oxytocin and Estrogen during Pregnancy and Parturition

Veiga¹,

Milazzotto²,

Nichi³

et al. 2016

American Journal of Animal and Veterinary Sciences

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Placenta is considered an important endocrine organ during gestation, as well as can also serve as a target organ for sex steroids and pituitary hormones, mainly for the cascade of events that trigger parturition. In order to initiate studies on the canine placental endocrinology regarding estrogen and oxytocin, the present study verified the correlation among the Estrogen Receptor alpha (ERα) and Oxytocin Receptor (OTR) gene expression in the placenta tissue with the serum concentrations of their respective hormones (estrogen and oxytocin) during canine pregnancy and parturition. Real-time PCR was performed to quantify the levels of ERα mRNA and OTR mRNA in the placenta tissue of bitches (n=46) during Early (up to 20 days of gestation; n=11), Mid (20 to 40 days; n=12) and Late Pregnancy (40 to 60 days; n=12) and Parturition (first stage of labor; n=11). Serum samples were collected for the evaluation of estradiol and oxytocin (OT) concentrations by radioimmunoassay. Placental ERα mRNA expression showed a progressive decrease along gestation towards parturition. Placental ERα mRNA expression was significantly higher until 40 days of pregnancy in comparison to Parturition. Regarding OTR mRNA expression, no statistical differences among groups were verified. The Parturition Group presented higher estrogen concentrations in relation to Early and Mid Pregnancy Groups. Serum oxytocin concentrations were not different among pregnancy and parturition. During late pregnancy, a positive correlation was observed between placental ERα mRNA and oxytocin serum concentration. In conclusion, ERα mRNA in the canine placental tissue varies in a time-dependent manner, especially during the period of placentation and placental growth. Conversely, serum estrogen concentrations do not regulate directly the changes in OT receptors expression in the canine placenta.

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Section: Introductionmentioning

confidence: 65%

Section: Real-time Pcr Amplificationmentioning

confidence: 99%

Correlation of Oxytocin (OTR) and Estrogen Receptor (ER) mRNA in the Canine Placenta with the Detected Circulating Levels of Oxytocin and Estrogen during Pregnancy and Parturition

Veiga¹,

Milazzotto²,

Nichi³

et al. 2016

American Journal of Animal and Veterinary Sciences

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“…In this study, we examined the local production of OXT and its regulation in human placenta. It is known that OXT levels in the blood increase during pregnancy (Thornton et al 1992, Veiga et al 2015, which was also Immunostaining of OXT and OXTR in human placental tissue. To examine localization of each protein, tissues were stained with specific antibodies for OXT (A) and OXTR (B).…”

Section: Discussionmentioning

confidence: 93%

The regulation of oxytocin and oxytocin receptor in human placenta according to gestational age

Kim

Lee

Kang

et al. 2017

Journal of Molecular Endocrinology

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Oxytocin (OXT) is a peptide hormone that plays a central role in the regulation of parturition and lactation. OXT signaling is mediated by OXT receptor (OXTR), which shows species- and tissue-specific expressions and gene regulation. In the present study, we examined the synthesis of OXT and OXTR in human placenta tissue according to gestational age. A total of 48 placentas were divided into early preterm, late preterm and term groups depending on gestational age, and expression of OXT and OXTR was evaluated. First, OXT and OXTR mRNA and protein were detected in normal placenta tissue via Q-PCR, Dot-blot and Western blot assay. Both OXT and OXTR levels in normal placenta increased gradually in the late stage of pregnancy, suggesting that local OXT may play a critical role in the function of the placenta. To determine the regulatory mechanism of OXT, placental BeWo cells were administrated estrogen (E2) or progesterone (P), and expression of OXT and OXTR was tested. The mRNA and protein levels of OXT and OXTR were upregulated by E2 but blocked by co-treatment with P In order to confirm the estrogen receptor (ESR)-mediated signaling, we administrated ESR antagonists together with E2 to BeWo cells. As a result, both OXT and OXTR were significantly altered by ESR1 antagonist (MPP) while moderately regulated by ESR2 antagonist (PHTPP). These results suggest that OXT and OXTR are controlled mainly by E2 in the placenta via ESR1 and thus may play physiological functions in the human placenta during the late stage of pregnancy.

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“…For example, in rats, the epithelial cells of the uterus can synthesize oxytocin ________________________________________________________________________________ ________________________________________________________________________________ Habib et al (2020) (Jarrett et al 2006). Furthermore, there is an indirect relationship between oxytocin and product of prostaglandins F2a (PGF2a) from endometrial cells (Veiga et al 2015).…”

Section: Uterusmentioning

confidence: 99%

Oxytocin: functions and activation

Habib

Al-Rishdy

Kassim

2020

Multi

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Ocitocina: funções e ativação RESUMO O objetivo desta revisão é descobrir a atividade da ocitocina em vários sistemas biológicos do corpo relacionados às funções reprodutivas. A ocitocina é um hormônio peptídico produzido no cérebro, armazenamento e secreção da hipófise na maioria dos animais, especialmente nos mamíferos. A ocitocina foi descoberta pela primeira vez por Henry Dale em 1906. Considerando que sua estrutura foi descoberta em 1952. A ocitocina possui uma ampla variedade de funções fisiológicas, como remoção de leite, comportamento materno, câncer e comportamento sexual nos dois tipos de sexo (masculino e feminino) , por exemplo, desempenha um papel no desempenho da excitação peniana, ejaculação, glândulas acessórias e nas contrações dos músculos lisos do útero e pélvica durante o acasalamento e parto, crescimento e desenvolvimento do nível do corpo lúteo e progesterona no sangue. Além disso, o efeito da ocitocina em alguns hormônios como a prolactina e o hormônio luteinizante. Além disso, existe uma relação entre ocitocina e tolerância ao estresse. Portanto, é importante e necessário entender os efeitos da ocitocina nas funções de alguém para fornecer informações sobre os processos biológicos do corpo que controlam esse hormônio. PALAVRAS-CHAVE: hormônio, remoção de leite, reprodução animal, tolerância ao estresse ABSTRACT The objective of this review is to uncover the activity of oxytocin in various biological systems in the body related to reproductive functions. Oxytocin is a peptide hormone produced in the brain, storage, and secretion from the pituitary gland in most animals especially the mammals. Oxytocin was discovered at first time by Henry Dale in 1906. Whereas, its structure was discovered in 1952. Oxytocin has a wide variety of physiological functions such as milk removal, maternal behavior, cancer and sexual behavior in both type sex (male and female), for example, its play a role in excitation penile, ejaculation, accessory glands performance and the contractions of smooth muscles of the uterus and pelvic during mating and parturition, growth and development of the corpus luteum and progesterone level in blood. Also, oxytocin effect on some hormones such as prolactin and luteinizing hormone. Furthermore, there is a relationship between oxytocin and stress tolerance. So, understanding the effects of oxytocin on somebody's functions is important and necessary to provide information about body biological processes which control by this hormone.

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Gene expression of estrogen and oxytocin receptors in the uterus of pregnant and parturient bitches

Cited by 17 publications

References 28 publications

Correlation of Oxytocin (OTR) and Estrogen Receptor (ER) mRNA in the Canine Placenta with the Detected Circulating Levels of Oxytocin and Estrogen during Pregnancy and Parturition

Correlation of Oxytocin (OTR) and Estrogen Receptor (ER) mRNA in the Canine Placenta with the Detected Circulating Levels of Oxytocin and Estrogen during Pregnancy and Parturition

The regulation of oxytocin and oxytocin receptor in human placenta according to gestational age

Oxytocin: functions and activation

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