Expression analysis of miRNA and target mRNAs in esophageal cancer

Meng, Xiangrui; Lü, Peng; Jiazhuan, Mei; Liu, G.J.; Fan, Qingxia

doi:10.1590/1414-431x20143906

Cited by 21 publications

(16 citation statements)

References 24 publications

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“…It has been reported that mutations of several genes are associated with esophageal cancer, such as p53, FasL and EGFR ( 6 – 9 ). Recent research has demonstrated that miRNAs play essential roles in esophageal cancer ( 10 ). MicroRNAs are a class of non-coding RNAs of 17–24 nucleotides, which regulate gene expression by targeting specific mRNAs ( 11 – 13 ).…”

Section: Introductionmentioning

confidence: 99%

miR-486 functions as a tumor suppressor in esophageal cancer by targeting CDK4/BCAS2

Lang

Zhao

2017

Oncol Rep

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Abstract. Esophageal cancer is a common tumor for which morbidity and mortality are high worldwide. We aimed to study alterations in miR-486 expression in esophageal cancers, and the effect miR-486 on esophageal cancer cell function and behavior. We collected esophageal cancer tissues/corresponding normal tissues from 20 patients and utilized three esophageal cancer cell lines and normal esophageal epithelial cells, and the expression of miR-486, CDK4 and BCAS2 was detected by qRT-PCR. Western blotting was used to detect the expression of CDK4 and BCAS2 protein. Then, we overexpressed miR-486 in esophageal cancer cell line EC9706. A series of cell functional analyses, including cell growth, cell cycle, apoptosis, migration and invasion were performed in esophageal cancer cells using colony formation assay, flow cytometry, Transwell and scratch assays, respectively. DualLuciferase reporter gene assay was used to detect the target genes of miR-486. We found that the expression of miR-486 in esophageal cancer tissues and cell lines was significantly lower than that in the normal tissues and normal esophageal epithelial cell line. Overexpression of miR-486 significantly inhibited the colony formation ability, induced G0/G1 phase arrest and apoptosis and suppressed cell migration and invasion in the EC9706 cells. Using bioinformatics and luciferase reporter assay, we identified that CDK4 and BCAS2 may be target genes of miR-486 and levels of CDK4 and BCAS2 were both significantly higher in the esophageal cancer tissues and cell lines than levels in the normal tissues and cells. Furthermore, knockdown of CDK4/BCAS2 coincided with the suppressive effects of miR-486 in esophageal cancer cells. Expression of apoptotic signaling molecules p21 and caspase-3 was upregulated in the CDK4/BCAS2-knockdown groups. These results suggest that miR-486 may suppress tumor cell growth and metastasis in esophageal cancer by targeting CDK4/BCAS2. The newly identified miR-486/CDK4/BCAS2 pathway provides further insight into the development and progression of esophageal cancer, which is of great significance to the early diagnosis and detection of esophageal cancer.

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Section: Introductionmentioning

confidence: 99%

miR-486 functions as a tumor suppressor in esophageal cancer by targeting CDK4/BCAS2

Lang

Zhao

2017

Oncol Rep

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“…The application of high-throughput sequencing and microarray technology has produced notable alterations in the miRNA expression profiles of tumor tissue, as compared with normal tissue (9,10). Variations in miRNA expression levels between tumor tissues and normal tissues may lead to the dysfunction of downstream signaling pathways, which are associated with proliferation, invasion, angiogenesis and the metastasis of tumors (11,12,13).…”

Section: Introductionmentioning

confidence: 99%

Low expression of microRNA-202 is associated with the metastasis of esophageal squamous cell carcinoma

Zhang

Dong

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. The present study aimed to determine the expression levels and biological functions of in patients with esophageal squamous cell carcinoma (ESCC). A total of 60 patients with ESCC and 30 healthy individuals were enrolled and reverse transcription-quantitative polymerase chain reaction was performed to measure the expression levels of miR-202. In order to investigate the effects of miR-202 expression levels on the proliferative, migratory and invasive abilities of ESCC cells, methylthiazolyl-tetrazolium bromide proliferation, in vitro scratch and Transwell ® chamber assays were performed. Expression levels of miR-202 were significantly decreased in the peripheral blood of patients with ESCC, which is associated with the degree of cell differentiation and lymph node metastasis (P<0.05). Following miR-202 transfection, cell proliferation was significantly inhibited (P<0.05). Cell migration and invasion was also significantly inhibited by miR-202 transfection (P<0.05). The results of the present study demonstrated that the expression of miR-202 inhibited the proliferation, migration and invasion of ESCC cells. Furthermore, low expression levels of miR-202 were detected in the peripheral blood of patients with ESCC, which is associated with the development, invasion and metastasis of ESCC.

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“…They also identified 576 upregulated probes and 1094 downregulated probes in ESCC samples (Fold change > 3; FDR < 0.001). Meng et al [ 14 ] reported four differently expressed miRNAs in ESCC tumor samples and normal tissues (FDR < 0.05), and 1110 differentially expressed genes (516 and 594 with decreased and increased expression, respectively, relative to their normal counterparts; FDR < 0.05).…”

Section: Resultsmentioning

confidence: 99%

“…If it was restricted to matched data set, we could not consider as many as samples in Table 1 . In addition to this, there were multiple published studies of miRNA–mRNA interaction employing unmatched data set [ 10 , 12 , 13 , 14 , 21 ]. Thus, using unmatched data solely cannot be the reason why the study should not be performed.…”

Section: Resultsmentioning

confidence: 99%

Identification of More Feasible MicroRNA–mRNA Interactions within Multiple Cancers Using Principal Component Analysis Based Unsupervised Feature Extraction

Taguchi

2016

IJMS

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MicroRNA(miRNA)–mRNA interactions are important for understanding many biological processes, including development, differentiation and disease progression, but their identification is highly context-dependent. When computationally derived from sequence information alone, the identification should be verified by integrated analyses of mRNA and miRNA expression. The drawback of this strategy is the vast number of identified interactions, which prevents an experimental or detailed investigation of each pair. In this paper, we overcome this difficulty by the recently proposed principal component analysis (PCA)-based unsupervised feature extraction (FE), which reduces the number of identified miRNA–mRNA interactions that properly discriminate between patients and healthy controls without losing biological feasibility. The approach is applied to six cancers: hepatocellular carcinoma, non-small cell lung cancer, esophageal squamous cell carcinoma, prostate cancer, colorectal/colon cancer and breast cancer. In PCA-based unsupervised FE, the significance does not depend on the number of samples (as in the standard case) but on the number of features, which approximates the number of miRNAs/mRNAs. To our knowledge, we have newly identified miRNA–mRNA interactions in multiple cancers based on a single common (universal) criterion. Moreover, the number of identified interactions was sufficiently small to be sequentially curated by literature searches.

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Expression analysis of miRNA and target mRNAs in esophageal cancer

Cited by 21 publications

References 24 publications

miR-486 functions as a tumor suppressor in esophageal cancer by targeting CDK4/BCAS2

miR-486 functions as a tumor suppressor in esophageal cancer by targeting CDK4/BCAS2

Low expression of microRNA-202 is associated with the metastasis of esophageal squamous cell carcinoma

Identification of More Feasible MicroRNA–mRNA Interactions within Multiple Cancers Using Principal Component Analysis Based Unsupervised Feature Extraction

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