Culture media for the multiplication of wild Manihot species

Sá, J. F. de; Sampaio, E. dos S.; Mendes, Maria Inês de Souza; Santos, Karen Cristina Fialho dos; Sousa, Antônio da Silva; Lêdo, Carlos Alberto da Silva

doi:10.1590/1413-70542018426024718

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…CVs for NS and PH varied from 22.14 to 29.24%, respectively. These values were less than those reported by Sá et al (2018). The latter authors reported CVs varying between 12.88 and 72.46% in the micropropagation of wild species of Manihot.…”

Section: Resultscontrasting

confidence: 56%

In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations

Santos

Silveira

Souza

et al. 2021

Acta Sci. Biol. Sci.

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In vitro multiplication is an important tissue culture technique that is capable of efficiently producing seedlings at any scale. It is a propagation method based on the aseptic culture of small propagules in a suitable culture medium to enable plant regeneration. Multiplication experiments conducted in vitro to set protocols adapted to wild Manihot species have used modified mineral salts and MS vitamins as basic culture medium. Here, 25 treatments based on combinations of the regulators benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) at 0, 0.025, 0.05, 0.075, and 0.1 mg L-1 were used for in vitro multiplication of three genotypes of wild Manihot species (M. violaceae Pohl Müll. Arg., M. pseudoglaziovii Pax & Hoff., and M. flabellifolia Pohl). Plant height and the number of 1 cm minicuttings, number of roots, shoots, green leaves and senescent leaves were recorded 120 days after explant inoculation. M. violaceae Pohl. Müll. Arg. and M. flabellifolia Pohl. presented favorable results with 0.05 and 0.025 mg L-1 NAA, respectively. Culture medium lacking NAA and BAP favored the in vitro growth of M. pseudoglaziovii Pax & Hoff.

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Section: Resultscontrasting

confidence: 56%

In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations

Santos

Silveira

Souza

et al. 2021

Acta Sci. Biol. Sci.

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“…This shows that, despite controlling cultivation conditions, data commonly needs transformation in order to follow the normality assumption (Moyo et al 2014), yet obtaining high CV values. Sá et al (2018), while studying micro-propagation of Manihot wild species, verified CV values form 12.88% to 72.46%.…”

Section: Discussionmentioning

confidence: 63%

Effect of different temperatures for in vitro conservation of cassava (Manihot esculenta Crantz) germplasm

Santos¹,

Freitas²,

Souza³

et al. 2022

CLIUM

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The objective of the present work was to evaluate in vitro growth of cassava accessions under different temperatures to improve protocols for germplasm conservation. Acclimatized chambers were tested at 18 °C, 20 °C and 22 °C, and again at 22 °C in conservation room (control) in five accessions: Mandioca João Grande (BGM1325), Fécula Branca (BGM1632), BRS Kiriris (BGM1728), S/N (121) (BGM2004) and BRS Prata (BGM2019). After 90 days of distance, the following were evaluated: shoot height (cm), number of sprouts and microcuttings, number of green and senescent leaves, fresh and dry weight of shoots and roots (g). The experiment was performed under a completely randomized design in factorial scheme 5(accessions) x 4(temperatures), with 20 replicates. All data were submitted to the F test ANOVA and evaluated by the Tukey and Scott-Knott tests at 5% probability. The control temperature promoted lower mean values for plant shoot height, with emphasis for accessions BGM1325, BGM1632 and BGM2019. In a general manner accessions BGM1325, BGM1632 and BGM2019 showed lower SH, with the first and BGM2004 outstanding with the best results for lower NSL and higher NGL.

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“…Kemampuan memperbanyak bibit melalui kultur jaringan sangat dipengaruhi oleh genotip tanaman, komposisi media, dan jenis serta konsentrasi zat pengatur tumbuh yang digunakan (Mathew et al, 2014). Media Murashige and Skoog (1962) merupakan media dasar yang banyak digunakan untuk regenerasi serta multiplikasi berbagai macam jenis tanaman secara in vitro termasuk ubi kayu (Khumaida et al, 2013;Sá et al, 2018). Penambahan jenis dan konsentrasi zat pengatur tumbuh (ZPT) yang tepat pada media sangat menentukan keberhasilan regenerasi tanaman.…”

Section: Pendahuluanunclassified

Pengaruh BA dan NAA terhadap multiplikasi tunas ubi kayu secara in vitro

Yelli¹,

Ardian²,

Utomo³

2022

J. AGRO

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Ubi kayu varietas UJ-3, klon BW-1 dan Unila UK-1 banyak ditanam khususnya di Lampung. Untuk itu diperlukan bibit dalam jumlah besar yang dapat dilakukan secara in vitro. Penelitian ini bertujuan untuk mengevaluasi pengaruh BA dan NAA terhadap induksi dan multiplikasi tunas ubi kayu secara in vitro. Penelitian dilakukan menggunakan rancangan acak lengkap (RAL) dengan dua faktor, yaitu : (1) Varietas/klon yang terdiri atas UJ-3 (K1), BW-1 (K2) dan Unila UK-1 (K3), dan (2) Media yang terdiri atas media Murashige and Skoog (MS) tanpa zat pengatur tumbuh (M1), MS + BA 0,1 mg L-1 (M2), MS + BA 0,1 mg L-1 + NAA 0,05 mg L-1 (M3), MS + BA 0,3 mg L-1 + NAA 0,05 mg L-1 (M4), MS + BA 0,5 mg L-1 + NAA 0,05 mg L-1 (M5). Setiap perlakuan diulang sebanyak empat kali. Hasil menunjukkan bahwa tunas dari varietas/klon ubi kayu dapat terinduksi pada semua media perlakuan. Rata-rata jumlah tunas tertinggi (1,25 tunas) dihasilkan pada media M4 untuk klon Unila UK-1, jumlah buku paling tinggi (8 buku) dihasilkan klon BW-1 pada media M3 dan M4, jumlah daun hijau tertinggi (6 helai) pada media M3. Klon BW-1 juga menghasilkan jumlah daun gugur tertinggi (4,5 helai) pada media M4. ABSTRACTCassava UJ-3, BW-1, and Unila UK-1 are widely planted especially in Lampung. Therefore, it needs a high number of planting materials that can be done through tissue culture. This study aimed to determine the effect of BA and NAA on in vitro of cassava shoot induction and multiplication. The study used a completely randomized design with two factors, namely: (1) Variety/clone which consisted of UJ-3 (K1), BW-1 (K2), and Unila UK-1 (K3), and (2) Medium which consisted of MS without growth regulators (M1), MS + BA 0,1 mg L-1 (M2), MS + BA 0,1 mg L-1 + NAA 0,05 mg L-1 (M3), MS + BA 0,3 mg L-1 + NAA 0,05 mg L-1 (M4) and MS + BA 0,5 mg L-1 + NAA 0,05 mg L-1 (M5). Each treatment was repeated four times. Results showed that shoots from all genotypes could be induced in all treatment mediums. The highest shoot number (1.25 shoots) was produced by Unila UK-1 on M4 medium. The BW-1 clone showed the best result in the node number (8 nodes) on the M3 and M4 medium, the green leaves number (6 sheets) on the M3 medium, and produced a high deciduous leaves number (4.5 sheets) on the M4 medium.

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Culture media for the multiplication of wild Manihot species

Cited by 5 publications

References 16 publications

In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations

In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations

Effect of different temperatures for in vitro conservation of cassava (Manihot esculenta Crantz) germplasm

Pengaruh BA dan NAA terhadap multiplikasi tunas ubi kayu secara in vitro

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