In vitro propagation of Campomanesia rufa: An endangered fruit species

Abstract

The Campomanesia rufa is a fruitful species native from Brazil considered as endangered by the IUC (International Union for Conservation of Nature) and low information regarding its propagation is available. In this context, the aim of the present study was to develop in vitro germination, micropropagation and callogenesis protocols for the species. For in vitro germination, seeds were inoculated in MS medium supplemented with GA3 (gibberellic acid) and for shoot induction, the medium was supplemented with three different cytokinins BA (benzyladenine), BAP (6-Benzylaminopurine) or TDZ (Thidiazuron). For shoot growth, culture medium containing BA, BAP, TDZ were maintained under white fluorescent lamps and blue:red light-emitting diodes (LED). GA3 was also tested on in vitro shoot elongation. For oxidation control, the medium was supplemented with PVP (polyvinylpyrrolidone) and for callus induction, 2.4-D (dichlorophenoxyacetic acid). The results showed 68% germination regardless of GA3 concentration. In the propagation stage, BAP at 4.5 µM induced a higher number of shoots (4.53) and LED lamps as the light source combined with the culture medium with 1 μM BAP induced a higher number of shoots (4.08). The highest average of C. rufa length (31.9 mm) was obtained using 8.8 µM GA3. The use of PVP (584.3 uM) controls up to 27.3% oxidation in young leaf explants. The use of 10 µM 2.4-D leads to a higher callus formation (58.7 %). Therefore, it can be concluded that the use of BAP is efficient in the induction of shoots, PVP controls oxidation leaf segments, and 2.4-D induces callus in C. rufa.

6-Benzylaminopurine and kinetin modulations during in vitro propagation of Quercus robur (L.): an assessment of anatomical, biochemical, and physiological profiling of shoots

“…Alterations in the antioxidant activity and content of photosynthetic Therefore, in addition to developing a micropropagation protocol, investigating the anatomical and physiological status of in vitro shoots may be crucial for further research. Many studies on the in vitro culture of woody plant species have focused on multiplication rates during micropropagation (Chalupa 1988;Sant'Ana et al 2018;Bhat et al 2022). In this way, many researchers have not considered the anatomical and physiological status of in vitro shoots at a deeper level.…”

Isolation, molecular identification, and in vitro control of Campomanesia rufa (O. Berg) Nied. endogenous bacteria

“…One species endemic to the Cerrado of Minas Gerais state (Villarroel et al, 2016) categorized as endangered by the (International Union for the Conservation of Nature -IUCN, 2023) is Campomanesia rufa (O. Berg) Nied., popularly known as 'casaqueira' or 'gabiroba' (Sant'Ana et al, 2018). The fruit of this woody Myrtaceae species has high vitamin C content and high in vitro antioxidant activity (Abreu et al, 2020).…”

“…However, in vitro multiplication is still difficult for many native Cerrado species such as C. rufa. Some of the reasons include low seed germination rate (Sant'Ana et al, 2018) and the lack of effective disinfection protocols to eliminate epiphytic and endophytic microorganisms naturally present in plants. The use of silver nanoparticles (AgNPs), known for their antibacterial properties, did not affect the in vitro multiplication of C. rufa nodal segments (Timoteo et al, 2019).…”

Germinação in vitro de <i>Dipteryx alata</i> Vogel (Fabaceae) e proliferação de brotos sob sistema de ventilação convencional e natural

“…Different plant growth regulators (PGRs) are used in micropropagation, including 6Benzylaminopurine (BAP) for shoot multiplication (SANT'ANA et al, 2018) and naphthalene acetic acid (NAA) for shoot growth or etiolation (KUMAR et al, 2016). However, it is important for these regulators to be correctly balanced to obtain satisfactory responses capable of influencing other stages of micropropagation, such as rooting or acclimation (KUMAR et al, 2016).…”