A Toxina Urêmica Ácido Guanidinicoacético Inibe O Metabolismo Oxidativo Dos Neutrófilos De Cães

Pereira, Paulo Roberto Mendes; Bosco, Álvaro Francisco; Almeida, Breno Fernando Martins de; Narciso, Luı́s; Ciarlini, Paulo César

doi:10.1590/1089-6891v16i428177

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…This decrease in neutrophilic oxidative metabolism and other changes that affect the phagocytic capacity of neutrophils, pointed out by Pereira et al (2015), indicates the immunosuppressive action of the uremic state in the nephropathic patient, as already demonstrated, in an in vitro environment, by PUBVET v.16, n.09, a1212, p.1-12, Set., 2022 Barbosa et al (2010) and Silva et al (2013) and demonstrated in dogs with uremic syndrome and in neutrophils of healthy dogs after use of uremic plasma (Tables 6 and 7) by Bosco et al (2017). In humans, impairment of adaptive immune activity has also been reported due to the uremic environment and to dialysis therapy and its complications (Table 8).…”

Section: Immunosuppression In Dogs With Stage III and Iv Of Ckdsupporting

confidence: 60%

Immune disorders in dogs with chronic kidney disease: Review

Sousa¹,

Souza²,

Vaz³

et al. 2022

Pubvet

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The aim of this literature review was to identify the stages of chronic kidney disease in dogs that have greater immune dysfunction and which cellular components of the innate and humoral immune system are most affected by the retention of uremic toxins. Chronic kidney disease is characterized by being a serious and irreversible disease, but common in the practice of medical clinic for dogs and cats and usually affects those of advanced age. The consequences of chronic kidney disease can expand to other systems as the disease progresses; systemic arterial hypertension, hypertensive retinopathies and secondary renal hyperparathyroidism are examples of extra-renal impairment caused by chronic kidney disease. The Interest Renal Society (IRIS), established degrees of progression of renal impairment according to serum concentrations of symmetrical creatinine and dimethylarginine, biomarkers of kidney injury, which allows aid in diagnosis, classification of the degree of nephrological and systemic impairment and treatment affected patient. Studies in human patients demonstrate that, in the terminal stages of kidney disease, there is an impairment of the immune function of these patients, which contributes to the increase in the rate of deaths related to secondary infections, cancers caused by viruses and low responsiveness to vaccines. In Veterinary Medicine, despite the scarcity of research on immunosuppression in dogs with chronic kidney disease, some have observed, in vitro and in vivo environments, dysfunction in the innate and humoral immune system of dogs with advanced chronic kidney disease, due to the proinflammatory environment resulting from the retention of uremic toxins that had their clearance affected by impaired kidney excretory function. Stage III of chronic kidney disease in dogs requires greater care, not only to prevent the disease from progressing to stage IV, but also to prevent immune disorders that may result in death. The adoption of intervention criteria and measures aimed at preventing and / or minimizing these immunological disorders depends on the expansion of research on this topic to establish better clinical management for dogs with nephrological disorder.

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Section: Immunosuppression In Dogs With Stage III and Iv Of Ckdsupporting

confidence: 60%

Immune disorders in dogs with chronic kidney disease: Review

Sousa¹,

Souza²,

Vaz³

et al. 2022

Pubvet

View full text Add to dashboard Cite

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“…It have been reported that MG inhibits neutrophil oxidative metabolism when activated by E. coli in humans, a fact not observed when the activation was performed with PMA or fMLP (Hirayama et al, 2000;Schepers et al, 2010). Guanidino compounds, such as guanidine-acetic acid, inhibit in vitro neutrophil oxidative metabolism from dogs (Pereira et al, 2015). Thus, we compared the in vitro effect of MG and uremic plasma, and showed that this toxin alone increased ROS production (superoxide and hydrogen peroxide) and accelerated the apoptosis of neutrophils from healthy dogs.…”

Section: Discussionmentioning

confidence: 94%

The uremic toxin methylguanidine increases the oxidative metabolism and accelerates the apoptosis of canine neutrophils

Bosco

Almeida

Pereira

et al. 2017

Veterinary Immunology and Immunopathology

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We investigated the hypothesis that the increased concentration of plasma methylguanidine (MG) increases oxidative metabolism and accelerates apoptosis of neutrophils from dogs with chronic kidney disease (CKD). To achieve this, the levels of MG were quantified in healthy (n=16) and uremic dogs with CKD stage 4 of according to the guidelines of the International Renal Interest Society (IRIS, 2015) (n=16) using high performance liquid chromatography (HPLC). To evaluate the isolated effect of MG on neutrophil oxidative metabolism and apoptosis, neutrophils isolated from 12 healthy dogs were incubated with the highest concentration of plasma MG (0.005g/L) observed in dogs with CKD. Neutrophil oxidative metabolism was assessed by flow cytometry, using the probes hydroethidine for superoxide production and 2',7'-dichlorofluorescein diacetate for hydrogen peroxide production, with or without phorbol myristate acetate (PMA) stimulus. Neutrophil apoptosis and viability were also evaluated in flow cytometer using the Annexin V-PE system, with or without the apoptosis-inducing effect of camptothecin. Uremic dogs presented higher concentrations of MG (p<0.0001), increased oxidative stress and primed neutrophils with higher apoptosis rate. The neutrophil abnormalities observed in vivo were also reproduced in vitro, using cells isolated from healthy dogs and incubated with MG. We obtained strong evidence that in dogs with CKD, increased MG levels contributed to oxidative stress and potentially compromised the non-specific immune response by altering the oxidative metabolism and viability of canine neutrophils.

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A Toxina Urêmica Ácido Guanidinicoacético Inibe O Metabolismo Oxidativo Dos Neutrófilos De Cães

Cited by 2 publications

References 15 publications

Immune disorders in dogs with chronic kidney disease: Review

Immune disorders in dogs with chronic kidney disease: Review

The uremic toxin methylguanidine increases the oxidative metabolism and accelerates the apoptosis of canine neutrophils

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