ALTERAÇÕES FISIOLÓGICAS E ATIVIDADE ENZIMÁTICA EM SEMENTES ARMAZENADAS DE Melanoxylon brauna Schott.

Borges, Eduardo Euclydes de Lima e; Flores, Andressa Vasconcelos; Ataíde, Glauciana da Mata; Matos, Antônio César Batista

doi:10.1590/01047760201521011569

Cited by 13 publications

(9 citation statements)

References 16 publications

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“…In this study, seeds were germinated in July, and at this time, the average temperatures were possibly below this value. However, Borges et al (2015), analyzing germination and vigor responses of M. brauna seeds (from Leopoldina, MG) to eight-month storage under relative humidity of 55, 75, and 93% and temperature of 20 °C, found that the germination speed and percentage decrease with increasing water level in the environment, and in storage environments with 75 and 93%, seeds tend to die due to stressful moisture conditions. Another possible explanation would be related to the natural geographical distribution of M. brauna.…”

Section: Discussionmentioning

confidence: 99%

Controlled-Release Fertilizer on Growth of Melanoxylon brauna Schott Seedlings

et al. 2019

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Melanoxylon brauna is a tree species native to the Atlantic Forest of high ecological and economic value. The objective of this study was to evaluate the growth and nutritional status of seedlings of two Melanoxylon brauna cultivars grown in substrate with different controlled-release fertilizer doses (CRF). The experiment was arranged in a randomized block 4 × 2 factorial design, with four CRF doses (0, 2, 4, and 8 kg m -3 ) and two seed origins (Viçosa-MG and Linhares-ES), with four replicates and 126 seedlings per plot. At 60, 90, and 120 days after emergence, seedlings were collected to measure variables related to emergence, growth, and nutrition. The greatest growth and best nutritional status were found for seedlings originated from seeds collected in Linhares at CRF dose of 8 kg m -3 . On the other hand, seedlings originated from seeds collected in Viçosa showed low emergence and high plant mortality.

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Section: Discussionmentioning

confidence: 99%

Controlled-Release Fertilizer on Growth of Melanoxylon brauna Schott Seedlings

et al. 2019

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“…The seeds stored in an environment with 55% relative humidity maintained similar germination and GSI values to the beginning of the test. Higher humidity caused a loss and physiological quality in the seeds, while in storage at 93% relative humidity the seeds lost germination at 4 months (Borges et al, 2015). This shows the importance of humidity and temperature for seed conservation.…”

Section: Discussionmentioning

confidence: 93%

Drying and storage of Melanoxylon brauna Schott. seeds

et al. 2021

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The objective of this work was to evaluate the sensitivity of Melanoxylon brauna Schott. tree legume seeds to desiccation and storage. In the drying experiment, the Melanoxylon brauna seeds were submitted to two drying conditions: a forced air circulation chamber (40.18 °C ± 0.13 and 28.48% ± 3.95 RH) and a silica gel desiccator (27.19 °C ± 1.28 and 26.19% ± 0.94 RH) for different times (0, 12, 24, 36, 72, and 144 hours). A completely randomized design in a 2 (drying methods) × 5 (drying times) factorial scheme plus control and 4 replications of 25 seeds was used. The following variables were evaluated before and after drying: seed moisture content, percentage of germinated seeds, germination speed index, percentage of mortality, normal and abnormal seedlings. In the storage experiment the seeds were divided into two batches: pre-dried (at 5.0% humidity) and without drying (control at 8.9% humidity). The seeds were then stored in plastic bags in three environments: refrigerator at 5 °C, freezer at –20 °C and room temperature (29 °C). The seeds were removed every four months and submitted to the humidity and germination test for 24 months. Data from this storage experiment were analyzed considering a randomized block design in a 2 (drying levels: presence and absence) × 3 (storage environments: refrigerator, freezer or room temperature) factorial scheme + 2 controls (with and without drying at baseline) and 4 repetitions of 25 seeds. Drying reduced initial seed water content from 8.9% to 5.0%, without loss of viability. Drying in the chamber at 40 °C was faster and more efficient than in silica gel. The results enable classifying the seeds of this species as orthodox, i.e. tolerant to desiccation. The fridge and freezer were efficient for storing the Melanoxylon brauna seeds up to 24 months, independent of previous drying, while storing the seeds at room temperature with previous drying makes them last longer than without drying, as the seeds can last up to 16 months with drying, or 12 months without drying.

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“…This suggests the participation of other enzymes in the process, such as the hydrolases endo-β-mannanase, α-galactosidases, polygalacturonases and pectin methylesterase, all of which contribute to the weakening of micropylar endosperm (Zhang;Chen;Xu, 2014;Bicalho et al, 2016). These enzymes also act in the weakening of the cell wall by mobilization of reserve material stored in the walls, thereby ultimately enabling and/ or contributing to radicle protrusion (Zhang;Chen;Xu, 2014;Borges et al, 2015). .…”

Section: Resultsmentioning

confidence: 99%

Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds

Oliveira

Souza

Silva

et al. 2020

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The endosperm of coffee beans is an important structure which is composed of different reserve compounds. In the present study, we followed anatomical features during the reserve mobilization of Coffea arabica L. cv 'Catuaí' seed samples, harvested at 20 different sites, after 0, 3, 6, 12, 24, 30 and 45 days of imbibition. Seed samples were properly stored for enzyme activity assessment and fixed in FAA solution (formaldehyde, acetic acid and ethanol, 1; 1; 18) and kept in ethanol 70% solution, for histochemical analysis. Alternatively, samples for phenolic compounds histochemical test were fixed in Ferrous sulfate in formalin. Fixed samples were cross-sectioned for detection of starch, lipids, essential oils, oleoresins, proteins, phenolic compounds, neutral polysaccharides, alkaloids, β-1,3 and β-1,4 glucans, and lignin. Overall, based on histochemical tests and enzyme activity, seed reserve mobilization was similar among the evaluated altitudes and mountainsides. During soaking, there is an intense consumption of reserve compounds, starting at the region close to the embryo. Reserve mobilization intensifies after root protrusion, from 12 days of soaking. The intensification of the reserve consumption concomitant with an increased activity of MDH is observed at the first 12 days, whereas SOD showed higher expression after 30 days of soaking. At the 30 th day, little reserve is observed in the endosperm. At 45 th day, cotyledon leaves are expanded, and the root system, constituted by the taproot and lateral roots, is well established.

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ALTERAÇÕES FISIOLÓGICAS E ATIVIDADE ENZIMÁTICA EM SEMENTES ARMAZENADAS DE Melanoxylon brauna Schott.

Cited by 13 publications

References 16 publications

Controlled-Release Fertilizer on Growth of Melanoxylon brauna Schott Seedlings

Controlled-Release Fertilizer on Growth of Melanoxylon brauna Schott Seedlings

Drying and storage of Melanoxylon brauna Schott. seeds

Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds

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