Ultrasound-Assisted Transesterification of Soybean Oil Using Combi-Lipase Biocatalysts

Freitas, Vitória Olave de; Matte, Carla Roberta; Poppe, Jakeline Kathiele; Rodrigues, Rafael C.; Ayub, Marco Antônio Záchia

doi:10.1590/0104-6632.20190362s20180455

Cited by 19 publications

(20 citation statements)

References 41 publications

(79 reference statements)

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“…The obtained results from Candida rugosa lipase nanoparticles as a biocatalyst revealed the high performance of nanoparticles for biodiesel production which may be attributed to the inclusion of methyl-β-cyclodextrin subsequently their crosslinking and conjugation, which providing enhancement of lipase enzyme activity and stability 37 . Additionally, a new technology, based on ultrasound application, has been employed for the improvement of enzymatic transesterification and enzymatic esterification processes for biodiesel production that may enhance oil dissolution, increase conversion rate, and prompt protein conformational changes that may enhance enzymatic catalytic activities 38 . However, ultrasound may lead to lipase enzyme deactivation 39 and consequently may cause inactivation of the enzymatic transesterification and enzymatic esterification processes and reduce the production yields of biodiesel.…”

Section: Introductionmentioning

confidence: 99%

Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

Najjar

Hassan

Zabermawi

et al. 2021

Sci Rep

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In this study, two highly thermotolerant and methanol-tolerant lipase-producing bacteria were isolated from cooking oil and they exhibited a high number of catalytic lipase activities recording 18.65 ± 0.68 U/mL and 13.14 ± 0.03 U/mL, respectively. Bacterial isolates were identified according to phenotypic and genotypic 16S rRNA characterization as Kocuria flava ASU5 (MT919305) and Bacillus circulans ASU11 (MT919306). Lipases produced from Kocuria flava ASU5 showed the highest methanol tolerance, recording 98.4% relative activity as well as exhibited high thermostability and alkaline stability. Under the optimum conditions obtained from 3D plots of response surface methodology design, the Kocuria flava ASU5 biocatalyst exhibited an 83.08% yield of biodiesel at optimized reaction variables of, 60 ○C, pH value 8 and 1:2 oil/alcohol molar ratios in the reaction mixture. As well as, the obtained results showed the interactions of temperature/methanol were significant effects, whereas this was not noted in the case of temperature/pH and pH/methanol interactions. The obtained amount of biodiesel from cooking oil was 83.08%, which was analyzed by a GC/Ms profile. The produced biodiesel was confirmed by Fourier-transform infrared spectroscopy (FTIR) approaches showing an absorption band at 1743 cm−1, which is recognized for its absorption in the carbonyl group (C=O) which is characteristic of ester absorption. The energy content generated from biodiesel synthesized was estimated as 12,628.5 kJ/mol. Consequently, Kocuria flava MT919305 may provide promising thermostable, methanol-tolerant lipases, which may improve the economic feasibility and biotechnology of enzyme biocatalysis in the synthesis of value-added green chemicals.

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Section: Introductionmentioning

confidence: 99%

Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

Najjar

Hassan

Zabermawi

et al. 2021

Sci Rep

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“…Ultrasound stirring and these optimized combilipases permitted it to get a biodiesel yield of about 90% when using soybean oil and 70% using the wasted oil after 18 h of reaction [393]. The same group used ultrasound stirring in the biodiesel production from methanol and soybean oil catalyzed by individual immobilized enzymes or the combilipase composed by the mixture of these immobilized enzymes, studying the effects of pulse conditions and ultrasonic amplitude [394]. The best results were obtained using an optimum combilipase formed by 10% Lipozyme TL IM, 15% Lipozyme RM IM and 75% Novozym 435, time pulse of 15 s, duty cycle of 50% and ultrasonic amplitude of 30%.…”

Section: Use Of Individually Immobilized Combilipases In Biodiesel Productionmentioning

confidence: 99%

“…The presence of tert-butanol did not improve the yields under ultrasounds stirring, while it did under mechanical stirring, suggesting that ultrasonic technology was enough to eliminate the diffusional problems. Under optimal conditions, the proposed combilipase produced 75% ethyl esters while the best individual lipase gave only 55% in 5 h [394].…”

Section: Use Of Individually Immobilized Combilipases In Biodiesel Productionmentioning

confidence: 99%

One Pot Use of Combilipases for Full Modification of Oils and Fats: Multifunctional and Heterogeneous Substrates

et al. 2020

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Lipases are among the most utilized enzymes in biocatalysis. In many instances, the main reason for their use is their high specificity or selectivity. However, when full modification of a multifunctional and heterogeneous substrate is pursued, enzyme selectivity and specificity become a problem. This is the case of hydrolysis of oils and fats to produce free fatty acids or their alcoholysis to produce biodiesel, which can be considered cascade reactions. In these cases, to the original heterogeneity of the substrate, the presence of intermediate products, such as diglycerides or monoglycerides, can be an additional drawback. Using these heterogeneous substrates, enzyme specificity can promote that some substrates (initial substrates or intermediate products) may not be recognized as such (in the worst case scenario they may be acting as inhibitors) by the enzyme, causing yields and reaction rates to drop. To solve this situation, a mixture of lipases with different specificity, selectivity and differently affected by the reaction conditions can offer much better results than the use of a single lipase exhibiting a very high initial activity or even the best global reaction course. This mixture of lipases from different sources has been called “combilipases” and is becoming increasingly popular. They include the use of liquid lipase formulations or immobilized lipases. In some instances, the lipases have been coimmobilized. Some discussion is offered regarding the problems that this coimmobilization may give rise to, and some strategies to solve some of these problems are proposed. The use of combilipases in the future may be extended to other processes and enzymes.

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“…The combination of a combined lipase and ultrasound has demonstrated significant innovation potential. Numerous studies should be conducted to optimize the use of this combined technology for biodiesel production [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Co-Immobilization of Rhizopus oryzae and Candida rugosa Lipases onto mMWCNTs@4-arm-PEG-NH2—A Novel Magnetic Nanotube–Polyethylene Glycol Amine Composite—And Its Applications for Biodiesel Production

Abdulmalek

Wang

et al. 2021

IJMS

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This article describes the successful synthesis of a novel nanocomposite of superparamagnetic multi-walled nanotubes with a four-arm polyethylene glycol amine polymer (mMWCNTs@4-arm-PEG-NH2). This composite was then employed as a support for the covalent co-immobilization of Rhizopus oryzae and Candida rugosa lipases under appropriate conditions. The co-immobilized lipases (CIL-mMWCNTs@4-arm-PEG-NH2) exhibited maximum specific activity of 99.626U/mg protein, which was 34.5-fold superior to that of free ROL, and its thermal stability was greatly improved. Most significantly, CIL-mMWCNTs@4-arm-PEG-NH2 was used to prepare biodiesel from waste cooking oil under ultrasound conditions, and within 120 min, the biodiesel conversion rate reached 97.64%. This was due to the synergy effect between ROL and CRL and the ultrasound-assisted enzymatic process, resulting in an increased biodiesel yield in a short reaction time. Moreover, after ten reuse cycles, the co-immobilized lipases still retained a biodiesel yield of over 78.55%, exhibiting excellent operational stability that is attractive for practical applications. Consequently, the combined use of a novel designed carrier, the co-immobilized lipases with synergy effect, and the ultrasound-assisted enzymatic reaction exhibited potential prospects for future applications in biodiesel production and various industrial applications.

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Ultrasound-Assisted Transesterification of Soybean Oil Using Combi-Lipase Biocatalysts

Cited by 19 publications

References 41 publications

Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

Optimizing the catalytic activities of methanol and thermotolerant Kocuria flava lipases for biodiesel production from cooking oil wastes

One Pot Use of Combilipases for Full Modification of Oils and Fats: Multifunctional and Heterogeneous Substrates

Co-Immobilization of Rhizopus oryzae and Candida rugosa Lipases onto mMWCNTs@4-arm-PEG-NH2—A Novel Magnetic Nanotube–Polyethylene Glycol Amine Composite—And Its Applications for Biodiesel Production

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