Bacterial Cellulose Production by Komagataeibacter hansenii ATCC 23769 Using Sisal Juice - An Agroindustry Waste

Abstract

-In this study, we have optimized production of bacterial cellulose (BC) by Komagataeibacter hansenii ATCC 23769 in a static cultivation using sisal juice, an agroindustrial residue, as substrate. Optimization of fermentation parameters has been carried out using the one-variable-at-a-time method. Effect of initial sugar concentration, pH, nitrogen supplement, and cultivation time was evaluated. The influence of nitrogen source and quantity for bacterial cellulose production was studied using a central composite rotational design (CCRD).The highest production of BC (3.38 g/L) was obtained after 10 days of cultivation, using sisal juice (pH 5) at 15 g/L of sugars and supplemented with 7.5 g/L of extract yeast. The cellulose production yield in selected sisal culture conditions was three times higher than the yield in synthetic medium, indicating that sisal juice is a suitable substrate for BC production.

Hydrogel bacterial cellulose: a path to improved materials for new eco-friendly textiles

“…It is abundantly present in the ecosystem, which makes it easy to obtain-by merely using various plants, as it is their main component. Unfortunately, cellulose obtained from wood has low crystallinity and is contaminated by other substances, like lignin and hemicellulose (Lima et al 2017). What is more, in case of plant-derived cellulose, its treatment and processing require the use of harmful chemicals, and for the decontamination process, a lot of energy and water is needed.…”

“…The most popular method is the use of alkaline treatment. During such a procedure, NaOH solutions of differing concentrations, from 0.5 to 5 M, are used (Dima et al 2017;Kulkarni et al 2012;Lima et al 2017;Bandyopadhyay et al 2018; Sederavičiūt_ e et al 2019). The alkaline purification method requires, however, the use of significant amounts of water and neutralizers to obtain materials with neutral pH.…”

Bacterial cellulose nanofiber-based films incorporating gelatin hydrolysate from tilapia skin: production, characterization and cytotoxicity assessment

“…The Hestrin & Schramm (HS) culture medium is usually used for BC production (Hestrin and Schramm 1954), although other synthetic media have been developed (Mohammadkazemi et al 2015). Several studies have evaluated low-cost media for BC production, such as fruit juices, syrups, molasses, coconut water, glycerol, waste products and others (Jahan et al 2017;Jozala et al 2016;Chawla 2009;Duarte et al 2015;Nascimento et al 2016;Lima et al 2017). The cashew crop peduncle (Anacardium occidentale, L.), a waste from cashew nuts processing, release citric acid and sugar when squeezed and despite the efforts, only 10% of the total waste produced is re-used in food manufacturing (Silveira et al 2012).…”

Production of bacterial cellulose using Gluconacetobacter kombuchae immobilized on Luffa aegyptiaca support

“…RV28 isolated from rotten fruits. Several authors have reported that BC production was more suitable when the medium pH was ranging from 5 to 6 54 , 55 . Thus, BC production using immobilized cell on LA support was higher than that in the absence of support.…”

“…Even though, consumption of ethanol and acetic acid in the medium might suddenly provide energy source in the biosynthetic path way of TCA cycle due to the liberation of ATP, thus, facilitating the fermentation process for BC production 78 ; the presence of acetic acid in the medium eventually decreased the pH leading to low concentration of BC. This might be due to the fact that the suitable pH range is 5–6 for the production of BC 54 . Ethanol in the production medium could be acting as a facilitator for the production of BC.…”