Optimization of the Operating Conditions for Rhamnolipid Production Using Slaughterhouse-Generated Industrial Float as Substrate

Borges, W. S.; Moura, Antonio; Filho, Ubirajara Coutinho; Cardoso, Vicelma Luiz; Resende, Miriam Maria de

doi:10.1590/0104-6632.20150322s00003675

Cited by 11 publications

(4 citation statements)

References 34 publications

(36 reference statements)

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“…1) indicated that in the case of glycerol in MSM, although the RL appeared at the same time with the microbial growth, production still increased after biomass reached a plateau. For this reason, the product formation appeared to be partly growth-associated as described by other authors [24]. However, in the case of soybean oil in MSM and SMSM, RL production began to increase after biomass reached a plateau and therefore, the production of RL in this case is typical of a secondary metabolite.…”

Section: Effect Of Different Media Componentssupporting

confidence: 67%

Optimization of Rhamnolipid Production by P. aeruginosa Isolate P6

El-Housseiny

Aboulwafa

Aboshanab

et al. 2016

J Surfact & Detergents

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Rhamnolipids are interesting microbial surfactants having great industrial importance. However, the main obstacles towards an economic production of rhamnolipids are low productivity and high raw‐material costs. Therefore, this study aimed at optimization of the culture media as well as culture conditions using response surface methodology for maximum rhamnolipid production by Pseudomonas aeruginosa isolate P6, a promising rhamnolipid‐producing isolate. The optimum medium for maximum rhamnolipid production was found to be a mineral salts medium with glycerol 2 % v/v as the carbon source. The optimum cultivation conditions using response surface methodology were found to be an incubation temperature of 30 °C, an agitation rate of 250 rpm, an inoculum size of 5 % v/v and unlike most studies, an initial pH of 7.5. The resulting model predicted data points that corresponded well to the experimental values. Optimization resulted in a threefold increase in rhamnolipid production reaching 7.54 g/L. The data are potentially useful for further industrial exploitation of rhamnolipid production by the studied isolate.

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Section: Effect Of Different Media Componentssupporting

confidence: 67%

Optimization of Rhamnolipid Production by P. aeruginosa Isolate P6

El-Housseiny

Aboulwafa

Aboshanab

et al. 2016

J Surfact & Detergents

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“…However, processes used to obtain purified products limit the rhamnolipids' application on an industrial scale [13]. Therefore, much research has been done for reducing costs and increasing yield by using cheap substrates [14], and optimizing production conditions [15]. The different process of production and screening natural strains and production strategies of genetic engineering needs more efficient methods for separating purifying rhamnolipids homologue.…”

Section: Introductionmentioning

confidence: 99%

Effect of Rhamnolipids on Pathogenicity Characteristics of Microorganisms in Organic Compost

Ghazaei

2021

RES MOL MED

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Background: Rhamnolipids are hydrophilic glycolipids, often classified as biosurfactants. They are produced by different bacterial species. Rhamnolipids are extensively studied in biological research because of their interesting features like antimicrobial, antifungal, and antiviral activities. Materials and Methods: To study the antimicrobial effect of rhamnolipid, we conducted a crosssectional study on the eight different pathogenic bacterial strains from November 2019 to June 2020. These bacterial strains were isolated from the organic compost. Both disk diffusion and broth microdilution methods were used to evaluate the inhibitory effects of rhamnolipids on these pathogenic bacteria. Also, protease and amylase enzyme activities were evaluated in these eight bacterial isolates. Results: For Bacillus stearothermophilus, within the area of 31.5 mm, no growth was observed, hence proving the inhibitory effect of rhamnolipid. After calculating the minimum inhibitory (MIC) and minimum lethal concentrations (MLC) for each bacterial strain, it has been found that the studied bacteria were more susceptible to rhamnolipids than most of the antibiotics. Strains were also quantified for their enzymatic activity of proteases and amylases. The bacterial strains of Bacillus stearothermophilus, Brevibacillus brevis, Bacillus licheniformis, and Bordetella petrii showed maximum protease activity. Whereas Escherichia coli, Enterococcus faecalis, Enterobacter aerogenes, and Pseudomonas aeruginosa showed high amylase activity. Conclusion: Rhamnolipids can be used as a potential antimicrobial agent for treatment of infections.

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“…Câmara jessicacamara.eq@gmail.com Rhamnolipids are a promising class of biosurfactants (Geys et al, 2014;Henkel et al, 2012). Rhamnolipids are mainly produced by Pseudomonas aeruginosa and represent one of the most important classes of biosurfactants because of lower environmental risks (Borges et al, 2015;Hassan et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Optimization and Characterization of Biosurfactant Rhamnolipid Production by Pseudomonas aeruginosa Isolated from an Artificially Contaminated Soil

Câmara

Sousa

Neto

2019

J Surfact & Detergents

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Biosurfactants are surfactants biologically produced by microorganisms, presenting several advantages when compared to synthetic surfactants. Pseudomonas aeruginosa is known for producing rhamnolipids, considered one of the most interesting types of biosurfactants due to their high yields, when compared to other types. In this work, the production of rhamnolipid from P. aeruginosa was optimized. At first, the Plackett–Burman design was used to select most significant variables affecting the biosurfactant production yield among nine variables—carbon–nitrogen ratio, carbon concentration, nitrogen source, pH, cultivation time, potassium and magnesium concentrations, agitation, and temperature. Then, using main variables, a central point experimental design aiming to optimize rhamnolipid production was performed. The maximum biosurfactant concentration obtained was 0.877 mg L−1. The rhamnolipid also displayed a great emulsification rate, reaching approximately 67%, and the ability to reduce water surface tension from 72.02 to 35.26 mN m−1 at a critical micelle concentration (CMC) of 127 mg L−1, in addition to presenting a good stability when exposed to wide pH and salinity ranges. The results suggest that rhamnolipids are promising substitutes for synthetic surfactants, especially due to lower impacts on the environment.

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Optimization of the Operating Conditions for Rhamnolipid Production Using Slaughterhouse-Generated Industrial Float as Substrate

Cited by 11 publications

References 34 publications

Optimization of Rhamnolipid Production by P. aeruginosa Isolate P6

Optimization of Rhamnolipid Production by P. aeruginosa Isolate P6

Effect of Rhamnolipids on Pathogenicity Characteristics of Microorganisms in Organic Compost

Optimization and Characterization of Biosurfactant Rhamnolipid Production by Pseudomonas aeruginosa Isolated from an Artificially Contaminated Soil

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