Optimization of Rhamnolipid Production by <i>P. aeruginosa</i> Isolate P6

El-Housseiny, Ghadir S.; Aboulwafa, Mohammad M.; Aboshanab, Khaled M.; Hassouna, Nadia A.

doi:10.1007/s11743-016-1845-4

Cited by 23 publications

(19 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, the extraction by chloroform was most commonly used for separation of RL so far, which has its limitation due to its high toxicity, while n-hexane, which has been extensively applied in vegetable oil extraction is a desired solvent. However, RL have been claimed to be insoluble in n-hexane (Heyd et al, 2008;Ramana and Karanth, 1989) so that n-hexane is commonly used to extract the residual oil from culture broth for determining the oil content during RL fermentation (El-Housseiny et al, 2016;Müller et al, 2010). As we previously noticed that the detected oil content was unreasonably high.…”

Section: Introductionmentioning

confidence: 94%

Extraction Separation of Rhamnolipids by n‐Hexane via Forming Reverse Micelles

Zhou

Sha

Long

et al. 2020

J Surfact & Detergents

View full text Add to dashboard Cite

Rhamnolipids (RL) have been regarded to be insoluble in n‐hexane. Unexpectedly, we have noticed that RL could be extracted together with vegetable oil by n‐hexane at analyzing oil content of fermentation broth. This paradoxic phenomenon was assumed to be due to the formation of reverse micelles. As found in this paper, the micelle size as well as conductivity increased due to water solubilization, illustrating the formation of reverse micelles of RL in n‐hexane. In this reverse micellar system, the maximal water solubility was detected to be 6.26 mol H2O/mol RL while the reverse critical micelle concentration of RL was around 3% (w/w). Hence, it seems that the presence of water could increase the dissolution of RL in n‐hexane via forming the reverse micelles of RL/n‐hexane/water. Lastly, the formation of reverse micelles was further applied for extraction of RL by n‐hexane. Using this method, over 99.0% of RL was extracted under the appropriate pH of 4.5. This extraction separation using n‐hexane instead of chloroform proposed a much cleaner and safer strategy in RL manufacture. Moreover, the capability of RL in forming reverse micelle system could benefit the future application on improving enzymatic reactions, stabilizing nanoparticles, environmental treatment, and protein folding, etc.

show abstract

Section: Introductionmentioning

confidence: 94%

Extraction Separation of Rhamnolipids by n‐Hexane via Forming Reverse Micelles

Zhou

Sha

Long

et al. 2020

J Surfact & Detergents

View full text Add to dashboard Cite

show abstract

“…The results of numerous reports indicate that rhamnolipid production by P. aeruginosa is dependent on medium components in addition to other factors (Mulligan and Gibbs 1989 ; Müller et al 2012 ; El-Housseiny et al 2016 , Gutiérrez-Gómez et al 2018 ), with carbon and nitrogen sources being among the most important factors to be considered (Huang et al 2018 ). To determine the potential of waste canola oil as a carbon source for rhamnolipid production, we used a factorial design to determine the effect of 3 parameters, carbon source (canola oil and waste canola oil), nitrogen source (ammonium sulfate and sodium nitrate) and production time (7 and 14 days) as well as the interaction between these parameters (Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

Use of waste canola oil as a low-cost substrate for rhamnolipid production using Pseudomonas aeruginosa

et al. 2019

View full text Add to dashboard Cite

Rhamnolipids are glycolipid biosurfactants that are primarily produced by Pseudomonas aeruginosa that have gained a great deal of interest for their numerous industrial applications and environmentally friendly properties. In this study, we explored the potential of waste canola oil as a low-cost and environmentally friendly substrate for the production of rhamnolipids by P. aeruginosa . Four different 2 3 full factorial designs were used to assess the effect of three independent factors on rhamnolipid production, including carbon source (canola oil and waste canola oil), nitrogen source [(NH 4 ) 2 SO 4 and NaNO 3 ] and production time (7 and 14 days). The highest observed yield was 3585.31 ± 66.24 mg/L when P. aeruginosa was cultured for 14 days with 3% v/v waste canola oil and 4 g/L of NaNO 3 . The nitrogen source proved to be a crucial factor, as the use of NaNO 3 rather than (NH 4 ) 2 SO 4 led to a 30-fold increase in production yield. The observed yield when waste canola oil was used was similar to, and even slightly higher than, that obtained using canola oil. Our results showed that waste canola oil has great potential for use as a carbon source for rhamnolipid production by P. aeruginosa , thus paving the way for the development of a low-cost, efficient, and environmentally friendly bioprocess for the production of rhamnolipids. Electronic supplementary material The online version of this article (10.1186/s13568-019-0784-7) contains supplementary material, which is available to authorized users.

show abstract

“…The GMSM (mineral salts medium (Bodour et al 2003 ) with 2% v/v glycerol added as the only carbon source, pH 7.5) was chosen as the optimum media for maximum production of RLs in our previous study (El-Housseiny et al 2016 ). Therefore, it was prepared and used in this study.…”

Section: Methodsmentioning

confidence: 99%

“…The production process was carried out using optimum conditions described in our previous study (El-Housseiny et al 2016 ). Briefly, 5% v/v of the seed culture was used to inoculate 50-mL aliquots of GMSM (containing 2% v/v glycerol as the carbon source) and incubated at 250 ± 2 rpm and 30 °C for 6 days.…”

Section: Methodsmentioning

confidence: 99%

“…Pseudomonas aeruginosa P6 is a promising RL producing strain isolated, identified and optimized for maximum RL production using glycerol containing media (GMSM) in our previous studies (El-Housseiny et al 2016 , 2017 ). Therefore, the goal of this study was to structurally characterize the RLs produced by this isolate, using thin layer chromatography (TLC), nuclear magnetic spectroscopy (NMR) and high performance liquid chromatography-mass spectrometry (HPLC-MS).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Structural and Physicochemical Characterization of Rhamnolipids produced by Pseudomonas aeruginosa P6

El-Housseiny¹,

Aboshanab²,

Aboulwafa

et al. 2020

AMB Expr

View full text Add to dashboard Cite

Rhamnolipids are important biosurfactants for application in bioremediation, enhanced oil recovery, pharmaceutical, and detergent industry. In this study, rhamnolipids extracted from P. aeruginosa P6 were characterized to determine their potential fields of application. Thin-layer chromatographic analysis of the produced rhamnolipids indicated the production of two homologues: mono- and di-rhamnolipids, whose structures were verified by 1H and 13C nuclear magnetic resonance spectroscopy. Additionally, high performance liquid chromatography-mass spectrometry identified seven different rhamnolipid congeners, of which a significantly high proportion was di-rhamnolipids reaching 80.16%. Rha-Rha-C10-C10 was confirmed as the principal compound of the rhamnolipid mixture (24.30%). The rhamnolipids were capable of lowering surface tension of water to 36 mN/m at a critical micelle concentration of 0.2 g/L, and exhibited a great emulsifying activity (E24 = 63%). In addition, they showed excellent stability at pH ranges 4–8, NaCl concentrations up to 9% (w/v) and temperatures ranging from 20 to 100 °C and even after autoclaving. These results suggest that rhamnolipids, produced by P. aeruginosa P6 using the cheap substrate glycerol, are propitious for biotechnology use in extreme and complex environments, like oil reservoirs and hydrocarbon contaminated soil. Moreover, P. aeruginosa P6 may be considered, in its wild type form, as a promising industrial producer of di-RLs, which have superior characteristics for potential applications and offer outstanding commercial benefits.

show abstract

Optimization of Rhamnolipid Production by P. aeruginosa Isolate P6

Cited by 23 publications

References 43 publications

Extraction Separation of Rhamnolipids by n‐Hexane via Forming Reverse Micelles

Extraction Separation of Rhamnolipids by n‐Hexane via Forming Reverse Micelles

Use of waste canola oil as a low-cost substrate for rhamnolipid production using Pseudomonas aeruginosa

Structural and Physicochemical Characterization of Rhamnolipids produced by Pseudomonas aeruginosa P6

Contact Info

Product

Resources

About