Evaluation of the extract of Zeyheria tuberculosa with a view to products for wound healing

Sarmento, Patrícia de Albuquerque; Ataíde, Terezinha da Rocha; Barbosa, Alexandra; Araújo-Júnior, João Xavier de; Lúcio, Ingrid Martins Leite; Bastos, Maria Lysete de Assis

doi:10.1590/0104-1169.3143.2385

Cited by 12 publications

(3 citation statements)

References 11 publications

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“…Therefore, the removal of factors (i.e., malnutrition, infection) that may adversely affect the normal wound healing period, as well as the use of substances capable of accelerating healing internally or externally is needed. Despite the allopathic hegemony and superiority of synthetic compounds for local and systemic use in wound repair, there is a growing interest in natural treatment options that support the wound healing process [6,7,8]. In this context, plants are regarded as a good source for various phytoconstituents, and these are assimilated more easily, as compared to synthetic drugs, by both humans and animals.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

et al. 2019

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Thymus sipyleus Boiss. subsp. rosulans (Borbas) Jalas (TS) is a commonly used plant in the treatment of various complaints, including skin wounds in Turkish folk medicine. Despite the widespread traditional use of TS, there is not any scientific report confirming the effectiveness of this plant on the healing process. This research aimed to investigate the effects of different extracts obtained from TS on biological events during wound healing, on a cellular basis. In this context, proliferative activities of the extracts, as well as the effects on wound closure and hydroxyproline synthesis, were determined. In addition to wound healing properties, the antioxidant, antibacterial and anti-inflammatory activities of the extracts were evaluated. Decoction (D) and infusion (I) extracts contained the highest amount of phenolic content and showed the most potent activity against DPPH radical. All extracts exhibited complete protection against the damage induced by hydrogen peroxide (H2O2) by increasing cell viability compared to only H2O2-treated groups, both in co-treatment and pre-treatment protocols. None of the extracts exhibited cytotoxic activity, and most of the extracts from the TS stimulated fibroblast proliferation and migration. All TS extracts exert anti-inflammatory activity by suppressing the overproduction of tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO). The most pronounced activity on hydroxyproline synthesis was observed in D extract. In summary, it was observed that TS extracts can promote the healing process by enhancing fibroblast migration, proliferation and collagen synthesis as well as suppressing pro-inflammatory cytokines. The obtained data in this work support the traditional use of TS as a valuable plant-based compound for the treatment of wounds.

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Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

et al. 2019

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“…The results of this study also corroborate with research that demonstrated that some popularly used medicinal plants could promote tissue repair mechanisms, justifying the potential of therapeutic use in the treatment of wounds, especially regarding the evaluation of its healing potential. This demonstrates the presence of phenolic compounds in the plant composition (Sarmento et al, 2014), obtained by the many extracts and essential oils present, and that the phenolic compounds can be effective for healing wounds (including chronic wounds) and burns (Działo et al, 2016).…”

Section: Discussionmentioning

confidence: 87%

Antioxidant effect of Hymenaea courbaril L (Jatob) sap on the healing of wounds on mice

Costa¹,

Martins²,

Peixoto³

et al. 2021

J. Med. Plants Res.

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The use of natural products with curative action is a common cultural practice in the Brazilian Amazon, but little explored scientifically. The effect of Hymenaea courbaril L sap extract and its contribution to wound healing in mice was evaluated. The antioxidant potential of the extract was studied using the radical 1,1-diphenyl-2-picrilhidrazil and 2,2'-azino-bis (3-ethyl benzothiazoline-6-sulfonic) acid radical, anion superoxide (O2-) radical tests, phenol and flavonoid content, proliferation assay, cell viability and migration. In vivo tests were performed on adult Swiss mice, submitted to back injury and treated with a formulation containing 2% sap extract, extracted with ethyl acetate. The animals were treated topically for 14 days, it was observed that the sap extract has antioxidant activity, inhibiting the production of reactive oxygen species, presented potential for the proliferation of fibroblasts and promoted cell migration. Morphometric analysis of wound closure on the 3rd day suggested that animals treated with Jatobá sap had a reduced area of injury when compared with the control group on the 3rd post-injury day for the treated group. On the 14th day, there was no difference between groups. The total closure of the wounds did not occur during the 14-day experiment, but through histological evaluation, it was found that there was re-epithelialization. The results suggest that Jatobá sap extract has the potential to induce the healing of skin wounds due to its antioxidant activity.

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“…us, we can infer that the low tannin content in the extracts of C. nutans leaves is not sufficient for it to have a good antimicrobial potential. e Artemia salina (A. Salina) toxicity test is a biological assay considered as one of the most widely used tools for preliminary toxicity assessment of plant extracts [84]. Extracts of plants with high toxicity against A. salina suggest high potential for biological activities, so it is very useful to use this bioassay in the direction of phytochemical studies in the search for bioactive substances [85].…”

mentioning

confidence: 99%

Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves

Souza

Gindri

Fortes

et al. 2020

Advances in Pharmacological and Pharmaceutical Sciences

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Context. Chaptalia nutans (L.) Pol. (family: Asteraceae) is widely used in traditional medicine as laxative and anticough medications and especially in the traumatisms, wounds, and hemorrhages in topical preparations. Objective. This work was to evaluate the chemical constitution of the hydromethanolic (30/70 methanol-water) macerating extract obtained from the leaves of C. nutans, as well as to study the antioxidant, antimicrobial, cytotoxic, and genotoxic activity of the species. Materials and methods. Phytochemical screening, antioxidant activity (total phenolic, total flavonoid, condensed tannins content, DPPH radical, and FRAP), antibacterial activity (P. aeruginosa, B. cereus, E. epidermidis, E. coli, S. aureus, E. faecalis, P. mirabilis, Candida glabrata (clinical isolate), Candida tropicalis (clinical isolate), C. krusei (clinical isolate), and C. albicans (clinical isolate)), and oxidative stress parameters (TBARS, carbonyl protein, and DCFH) were analyzed according to the literature. Toxicity of C. nutans was evaluated using an alternative method, D. melanogaster, as well as a locomotor assay. Results. The phytochemical screening test of methanolic leaves extract revealed the presence of alkaloids, coumarins, quaternary bases, phenolics, flavonoids, tannins, and free steroids. A quantitative phytochemical study indicated the total phenol (30.17 ± 1.44 mg/g), flavonoid (21.64 ± 0.66 mg/g), and condensed tannins (9.58 ± 0.99 mg/g). DPPH (345.41 ± 5.35 μg/mL) and FRAP (379.98 ± 39.25 μM FeSO4/mg sample) show to extract of C. nutans leaves an intermediate value, indicating moderate antioxidant activity of the extract. Antibacterial results revealed only a positive result (antimicrobial activity) for the hexane fraction which significantly inhibited the microorganisms E. epidermidis, C. tropicalis, C. glabrata, and C. krusei at a concentration of 1000 μg/mL. TBARS, carbonyl protein, and DCFH demonstrate that the extract has the ability to protect the cell from protein and lipid damage, as well as the inhibition of oxygen-derived radicals at the three concentrations tested: 0.1, 1, and 10 mg/mL. Regarding the toxicity of C. nutans extract against D. melanogaster, it was found that until the concentration of 15 mg/mL, the extract showed no toxicity and that the LC50 obtained was 24 mg/mL. Results show that the C. nutans extract leaves used to prevent PQ damage were effective in reducing flies’ mortality and improving locomotor capacity. Conclusion. Our studies demonstrated for the first time that C. nutans crude leaf extract has high antioxidant capacity both in vitro and in vivo through different analysis techniques. These results make it possible to infer future applications in the pharmacological area, evidenced by the low toxicity observed in D. melanogatser, as well as the ability to neutralize different sources of RONS.

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Evaluation of the extract of Zeyheria tuberculosa with a view to products for wound healing

Cited by 12 publications

References 11 publications

In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

Antioxidant effect of Hymenaea courbaril L (Jatob) sap on the healing of wounds on mice

Phytochemical Analysis, Antioxidant Activity, Antimicrobial Activity, and Cytotoxicity of Chaptalia nutans Leaves

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