Immunomodulatory action of jacalin from Artocarpus integrifolia and mannoprotein from Saccharomyces uvarum on the humoral immunity of laying hens

Abstract

Advances in the fields of glycobiology and immunology have provided many insights into the role of carbohydrate-protein interactions in the immune system. Jacalin of Artocarpus integrifolia (JCA) and structural mannoprotein of Saccharomyces uvarum (MPS) are molecules with immunomodulatory properties. JCA is an IgA human lectin binding molecule that causes the mitogenic stimulation of immune cells, production of cytokines, chemotaxis, and activation of leukocytes. Studies on the immunomodulatory properties of JCA and MPS in mammals and fish suggest that they have an action on antibody production. The aim of this study was to investigate the possible action of JCA and MPS on the production of specific antibodies in laying hens. For this, laying hens were inoculated with an intra abdominal injection of sheep red blood cells (SRBC) with either JCA (0.075 µg, 0.75 µg, and 7.5 µg) or MPS (20 µg and 100 µg). Levels of anti-SRBC antibodies of the IgY, IgM, and IgA classes were evaluated by ELISA. Results showed that JCA and MPS have immunomodulatory effects on levels of anti-SRBC IgM, IgA, and IgY. An immunostimulatory effect of JCA was observed in primary immune response on anti-SRBC IgY, while an inhibitory effect of JCA and MPS was observed in secondary immune response on the production of IgM and IgA anti-SRBC. These results suggested that MPS and JCA have immunomodulatory effects on antibody production and could be used in future studies on humoral immune response in poultry.

Efeito da lectina ArtinM em células B de origem murina e células de linhagem humana de linfoma não-Hodgkin

“…Neste estudo, os autores identificaram os principais receptores-alvo para Gal-9 nas células B. Como resultado, mostraram que o efeito regulatório de Gal-9 em células B naive é devido ao reconhecimento de glicanos expressos em CD45 (GIOVANNONE et al, 2018b). Diferentes estudos têm descrito o efeito imunomodulador de Jacalina, uma lectina isolada de sementes de Artocarpus heterophyllus (jaca), com especificidade para ligação a Galβ1-3GalNAc (GABRIELA et al, 2013;JANDÚ et al, 2017;SILVA et al, 2020). Resumidamente, CD45 é relatado como o principal alvo da jacalina nas células B, promoveu a mobilização do Ca2 + intracelular e induziu a ativação da calpaína, efeito que levou à morte por apoptose (MA et al, 2009).…”