Creole bean seeds microbiolization with doses of Trichoderma harzianum

Abstract

In the search for improved yields, seed treatment by microbiolization has been used as an alternative to chemical treatment. The objective was to verify the physiological and sanitary quality of creole bean seeds, var. Chumbinho, after microbiolization with doses of a commercial product (c.p.) with Trichoderma harzianum (strain ESALQ-1306). The treatments were: T1) 100 mL c.p./100 kg seeds; T2) 150 mL c.p.; T3) 200 mL c.p.; T4) 200 mL of chemical treatment (c.p., 250 g L-1 fipronil + 25 g L-1 pyraclostrobin + 225 g L-1 thiophanate-methyl); and T5) control (without coating of seeds). The tests were: sanitary test (blotter test); germination and first count; accelerated aging, cold germination without soil, speed of germination rate (SGR), seedling shoot and root lengths, and emergence of seedlings in a greenhouse. T. harzianum controlled Aspergillus spp., Penicillium spp. and Fusarium oxysporum. With 100 mL c.p. of T. harzianum dose had better results for the germination and vigor, and this dose it is an alternative to chemical treatment in creole bean seeds.

Germination and Agronomic Traits of Phaseolus vulgaris L. Beans Sprayed with Trichoderma Strains and Attacked by Acanthoscelides obtectus

“…and seeds in the early stages of germination [65], or the dosage to be applied. Dalzotto et al [64] obtained a high germination percentage in P. vulgaris seeds treated with Trichoderma. Another example is the inoculation of tomato seeds with T. harzianum (Rifai T-22 strain) through a conidia solution of 2 × 10 7 colony forming units per gram of seeds, which had a positive effect on seed germination and growth in in vitro culture [65].…”

“…Regardless of the seed condition, the application of ∆tri23 provided plants with a great wet weight aerial part, in spite of its poor percentage of germination in comparison to the control or to the rest of the strains applied, whereas high germination rates of Ta37 and ∆tri17 (greater than their controls or than the rest of the assessed strains) did not lead to great wet weight aerial part or wet weight root system values in the plants obtained. The use of biological control agents is one of the alternatives for seed treatment to reach greater sustainability in agriculture [63,64]. Trichoderma strains are widely used in seed treatment, but little is known about the possible interactions between Trichoderma spp.…”

“…Another example is the inoculation of tomato seeds with T. harzianum (Rifai T-22 strain) through a conidia solution of 2 × 10 7 colony forming units per gram of seeds, which had a positive effect on seed germination and growth in in vitro culture [65]. The improvement in the germination of seeds treated by Trichoderma strains may be due to the production of hormones from Trichoderma [64]. T. harzianum produces harzianic acid and isoharzianic acid, which promote plant growth [66].…”