Antioxidant potential of various solvent extract from Morus alba fruits and its major polyphenols composition

Kobus‐Cisowska, Joanna; Szczepaniak, Oskar; Szymanowska, Daria; Piechocka, Justyna; Szulc, Piotr; Dziedziński, Marcin

doi:10.1590/0103-8478cr20190371

Cited by 36 publications

(27 citation statements)

References 32 publications

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“…A direct relation was observed between extract yields and polyphenols which might appear in antioxidant and biological activities. The strong linkage between phytochemicals present in plant extracts and antioxidant activity is well reported and established phenomenon [20,25].…”

Section: Extract Yield Total Phenolic and Flavonoid Contentsmentioning

confidence: 95%

Anti-Obesity Attributes; UHPLC-QTOF-MS/MS-Based Metabolite Profiling and Molecular Docking Insights of Taraxacum officinale

et al. 2020

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The naturopathic treatment of obesity is a matter of keen interest to develop efficient natural pharmacological routes for disease management with low or negligible toxicity and side effects. For this purpose, optimized ultrasonicated hydroethanolic extracts of Taraxacum officinale were evaluated for antiobesity attributes. The 2,2-diphenyl-1-picrylhydrazyl method was adopted to evaluate antioxidant potential. Porcine pancreatic lipase inhibitory assay was conducted to assess the in vitro antiobesity property. Ultra-high performance chromatography equipped with a mass spectrometer was utilized to profile the secondary metabolites in the most potent extract. The 60% ethanolic extract exhibited highest extract yield (25.05 ± 0.07%), total phenolic contents (123.42 ± 0.007 mg GAE/g DE), total flavonoid contents (55.81 ± 0.004 RE/g DE), DPPH-radical-scavenging activity (IC50 = 81.05 ± 0.96 µg/mL) and pancreatic lipase inhibitory properties (IC50 = 146.49 ± 4.24 µg/mL). The targeted metabolite fingerprinting highlighted the presence of high-value secondary metabolites. Molecular-binding energies computed by docking tool revealed the possible contribution towards pancreatic lipase inhibitory properties of secondary metabolites including myricetin, isomangiferin, icariside B4, kaempferol and luteolin derivatives when compared to the standard drug orlistat. In vivo investigations revealed a positive impact on the lipid profile and obesity biomarkers of obese mice. The study presents Taraxacum officinale as a potent source of functional bioactive ingredients to impart new insights into the existing pool of knowledge of naturopathic approaches towards obesity management.

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Section: Extract Yield Total Phenolic and Flavonoid Contentsmentioning

confidence: 95%

Anti-Obesity Attributes; UHPLC-QTOF-MS/MS-Based Metabolite Profiling and Molecular Docking Insights of Taraxacum officinale

et al. 2020

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“…There is great variation in the extract composition depending on the solvent system used. The phenolic content and consequently the antioxidant activity of natural extracts depend on the solvent used for extraction (KOBUS-CISOWSKA et al, 2020).…”

Section: Spray Dryingmentioning

confidence: 99%

“…These compounds are potent free radical scavengers, donating hydrogen, and their high antioxidant potential is related to their large number of phenolic hydroxyls. Fruits are the main dietary sources of polyphenols; however, the composition of these constituents in these foods is diversified according to variety, cultivar, edaphoclimatic conditions, among others (SILVA et al, 2012;KOBUS-CISOWSKA et al, 2020). Teixeira, Stringheta and Oliveira (2008) compared the method for quantification of anthocyanins, single pH and differential pH, and found 64.06 and 56.75 mg 100g -1 of sample, respectively, for eggplant, using ethanol-water as solvent (70:30) acidified with HCl to pH 2.…”

Section: Introductionmentioning

confidence: 99%

Stability of encapsulated and non-encapsulated anthocyanin in yogurt produced with natural dye obtained from Solanum melongena L. Bark

et al. 2020

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Abstract This research evaluated the stability of non-encapsulated and spray-dried encapsulated anthocyanin from Solanum melongena L. bark as a natural dye in yogurts. Anthocyanin was extracted using 70% cereal alcohol acidified with citric acid at pH 2.0. Extract spray-drying was defined using the experimental design (22), with drying temperature (°C) and carrier concentration (Gum arabic) as independent variables. The response variable adopted was the total monomeric anthocyanin content. The following analyses were performed: color; total monomeric anthocyanins; total phenolic contents; antioxidant activity using the DPPH• (1,1-diphenyl-2-picrylhydrazyl) radical; pH; and acidity. Yogurt degradation constant, half-life, and color retention percentage of anthocyanin added to yogurt were calculated. The total monomeric anthocyanin value found in the extract was 67.21 mg 100g-1 of bark. Natural non-encapsulated and encapsulated dye extract were added to yogurt at concentrations of 1.0; 1.5; and 2 g extract/100 mL yogurt. Average pH values after addition of anthocyanin extract ranged from 3.60 to 3.87. Acidity was 1.04% in natural yogurt (time zero), while 1%; 1.5%; and 2% samples showed 1.4; 1.7; and 2.05% and 1.18; 1.29; and 1.47% acidity in non-encapsulated extract added to yogurt and encapsulated extract added to yogurt, respectively. Anthocyanin content decreased during storage using non-encapsulated extract, remaining constant in encapsulated extract added to yogurt. The three formulations showed increased L* at thirty days, with discoloration being related to decreased anthocyanin content. DPPH• radical scavenging activity decreased during the 30 days of storage. The content of phenolic compounds ranged from 106.01 to 239.90 mg GAE 100 g-1 of yogurt in non-encapsulated extract added to yogurt, and from 225.17 to 291.98mg GAE 100 g-1 of encapsulated extract added to yogurt. Extract encapsulation is indicated because it provides high anthocyanin stability.

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“…The total phenolic content (TPC) was conducted according to the spectrophotometric method described in Kobus-Cisowska et al [ 16 ]. The absorbance of 725 nm was recorded by Metertech SP-830 apparatus (Taiwan).…”

Section: Methodsmentioning

confidence: 99%

“…The metal chelating ability of the chocolates were estimated according to the method described by Kobus-Cisowska et al [ 16 ] based on the absorbance measurement (562nm) of the complex (A1) of Fe 2+ ions not previously bonded to the extracts and 3-(2-pyridyl)-5,6-bis(4-phenyl-sulfonicacid)-1,2,4-triazine (Ferrozine, Sigma Aldrich, Poland). The final results were determined as % of the chelating activity (ChA) according to Eq 3 .…”

Section: Methodsmentioning

confidence: 99%

Can cornelian cherry mask bitter taste of probiotic chocolate? Human TAS2R receptors and a sensory study with comprehensive characterisation of new functional product

et al. 2021

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Cornelian cherry (Cornus mas L.) fruits are a valuable source of bioactive compounds that are responsible for the perception of bitter taste of chocolate products. The aim of the study was to validate the inhibitory effect of Cornus mas on the TAS2R3 and TAS2R13 bitter taste receptors and to assess the effect of masking the bitter taste of dark chocolate with the help of the sensory panel. Dark chocolate was prepared with an addition of 5% of freeze-dried cornelian cherry fruits and 108 CFU/g of Bacillus coagulans probiotic strains. Effect on the TAS2R receptors was evaluated in specially transfected HEK293T cells, and the inhibition ratio was measured using the calcium release test. Moreover, the total polyphenol content, antioxidant activity and simulated intestinal in vitro digestion were determined for the samples. The tested chocolate products were rich in chlorogenic, caffeic and sinapic acids. The addition of cornelian cherry positively affected the antioxidant activity. The phytochemicals of Cornus mas decreased the TAS2R13 activity by 132% after a 2-minute interaction and, % at the same time, inhibited the TAS2R3 activity by 11.5. Meanwhile, chocolate with the addition of fruit was less bitter according to the sensory panel.

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Antioxidant potential of various solvent extract from Morus alba fruits and its major polyphenols composition

Cited by 36 publications

References 32 publications

Anti-Obesity Attributes; UHPLC-QTOF-MS/MS-Based Metabolite Profiling and Molecular Docking Insights of Taraxacum officinale

Anti-Obesity Attributes; UHPLC-QTOF-MS/MS-Based Metabolite Profiling and Molecular Docking Insights of Taraxacum officinale

Stability of encapsulated and non-encapsulated anthocyanin in yogurt produced with natural dye obtained from Solanum melongena L. Bark

Can cornelian cherry mask bitter taste of probiotic chocolate? Human TAS2R receptors and a sensory study with comprehensive characterisation of new functional product

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