Micropropagation of Stevia rebaudiana plants

Rokosa, Marta; Kulpa, Danuta

doi:10.1590/0103-8478cr20181029

Cited by 7 publications

(2 citation statements)

References 26 publications

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“…A synergistic effect of cytokinin in combination with auxins was reported for Clematis heynei M.A. Rau [1], and Stevia rebaudiana Bertoni [16,17]. However, Gao et al [18] achieved an effective adventitious bud proliferation of Clematis 'Julka' using MS medium supplemented with 1 mg•dm −3 BAP + 0.2 mg•dm −3 IBA and 0.2 mg•dm −3 GA 3 .…”

Section: Resultsmentioning

confidence: 99%

Propagation of Clematis ‘Warszawska Nike’ in In Vitro Cultures

Kulpa,

Krupa-Małkiewicz

2023

Agronomy

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A micropropagation protocol for growing Clematis ‘Warszawska Nike’ was developed. The MS medium supplemented with 1 mg∙dm−3 BAP showed good results in the case of microshoot initiation (80%). The addition of BAP to the medium at higher concentrations resulted in the formation of a large amount of callus tissue at the base of the explant. Of the explants growing on the medium with the lowest cytokinin concentration, 8% flowered. Very quickly, after just 14 days, the explants began to die: some of the leaves that developed in in vitro cultures began to turn yellow and wither. The propagation of shoots was performed in two steps. In the first step, cytokinin BAP and Kin in various concentrations (0.5–2 mg∙dm−3) were added to the MS medium. In the second step, MS medium with the combinations of BAP (0.5 and 1 mg∙dm−3) with IAA or GA3 (1 and 2 mg∙dm−3) was used. The MS medium with 0.5 mg∙dm−3 BAP and 2 mg∙dm−3 GA3 was the best medium for the multiplication stage of clematis. Plants growing on this medium had the largest number of leaves, shoots, and internodes, and were also heavier compared to plants propagated on other media. The proliferated clematis explants were rooted on MS medium with the addition of IAA or IBA in different concentrations (0.5 to 4 mg∙dm−3). Of the concentrations tested, 0.5 mg∙dm−3 IAA was the most effective one for in vitro root induction. The highest percentage of acclimatized plants (75%) was observed when the shoots were rooted on MS medium with 0.5 mg∙dm−3 IAA.

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Section: Resultsmentioning

confidence: 99%

Propagation of Clematis ‘Warszawska Nike’ in In Vitro Cultures

Kulpa,

Krupa-Małkiewicz

2023

Agronomy

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“…Penambahan BAP 0,5 mg/L pada penelitian ini menghasilkan pertumbuhan tunas yang terbaik dibandingkan dengan perlakuan media lainnya. Hasil penelitian ini sejalan dengan penelitian Rokosa & Kulpa (2020), media MS yang ditambahkan BAP 1 mg/mL menghasilkan jumlah tunas yang tertinggi dibandingkan dengan BAP dengan konsentrasi yang lebih tinggi, 2iP dan kinetin dengan konsentrasi masing-masing 0,5; 1; 2; 5 mg/mL. Tunas yang dihasilkan dalam jumlah banyak sangat menguntungkan untuk perbanyakan benih stevia.…”

Section: Hasil Dan Pembahasan Induksi Tunasunclassified

Mikropropagasi Pada Tanaman Stevia rebaudiana (Bertoni)

Parnidi

Ridhawati

2020

Bul. Tan. Tembakau, Serat & Minyak Industri

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Stevia merupakan salah satu tanaman penghasil pemanis alami. Mikropropagasi stevia melalui kultur jaringan dapat menyediakan bahan tanaman secara massal dan cepat yang diperlukan untuk pengembangan stevia. Pada mikropopagasi melalui kultur jaringan diperlukan komposisi media yang tepat. Penelitian ini bertujuan untuk menguji komposisi media yang sesuai untuk mikropropagasi tanaman stevia. Penelitian dilakukan di Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Pemanis dan Serat pada Februari-Juni 2016. Penelitian dilakukan menggunakan Rancangan Acak Lengkap (RAL) setiap perlakuan diulang tiga kali. Induksi tunas stevia menggunakan media dasar Murashige and Skoog (MS) dengan penambahan Benzil Amino Purin (BAP) dengan konsentrasi 0; 0,25; 0,5; 0,75 dan 1 mg/L. Induksi perakaran stevia menggunakan media dasar MS dengan dengan penambahan 1; 1,5; 2 dan 2,5 mg/L IAA, IBA dan NAA dan media MS tanpa penambahan ZPT sebagai kontrol. Hasil penelitian menunjukkan bahwa media MS + BAP 0,5 mg/L menunjukkan pertumbuhan terbaik dengan rerata jumlah tunas 17,80 dan rerata panjang tunas 3,25 cm. Media perakaran terbaik terdapat pada perlakuan media MS + IAA 1mg/L yang menghasilkan jumlah akar dengan rerata 4,60 dan panjang akar 2,27 cm. Kata kunci: Stevia, induksi tunas, induksi perakaran, in vitro Micropropagation on Stevia rebaudiana (Bertoni)

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