Mineral composition, histomorphometry, and bone biomechanical properties are improved with probiotic, prebiotic, and symbiotic supplementation in rats chronically exposed to passive smoking: a randomized pre-clinical study

Abstract

Cigarette smoke in large centers is one of the most important causes of chronic inflammatory diseases in public health and is associated with a decrease in bone mass, consolidation process, and bone remodeling. Due to their ability to improve intestinal absorption and compete with pathogenic microorganisms, dietary supplementation with functional foods may contribute to improvement in bone quality. Therefore, the objective of this study was to evaluate the effects of functional, probiotic, prebiotic, or symbiotic food supplementation on mineral composition, histomorphometry, and bone biomechanical properties of rats in the growth phase, chronically exposed to cigarette smoke (T).Sixty-four young male rats were randomly assigned to eight groups (n=8): control (C) [standard diet (SD)]; probiotic (Pro) [SD + probiotic (Lactobacillus acidophilus, Enterococcus faecium, Bifidobacterium thermophilum and Bifidobacterium longum) (2-5 109 UFC each)]; prebiotic (Pre) [SD+ prebiotic (mannan oligosaccharide)]; symbiotic (Sym) (SD + probiotic + prebiotic); control smoking (SC) [(SD + exposure protocol to passive smoking (PS)]; probiotic smoking (ProS) (SD + probiotic + PS); prebiotic smoking (PreS) (SD + prebiotic + PS), and symbiotic smoking (SymS)(SD + prebiotic + probiotic + PS). The animals were euthanized after 189 days of the experimental protocol. Results showed that supplementation with probiotics, prebiotics, and symbiotics significantly improved (P<0.05) the parameters: P, Ca, Mg, BMD, BMC, strength, resilience, and size of area of the femoral diaphysis of the animals chronically exposed or not cigarette smoke. We concluded that functional food supplementation improved the bone health of rats chronically exposed or not to cigarette smoke.

The Impact of Multispecies Probiotics on Calcium and Magnesium Status in Healthy Male Rats

“…The elevation of the proliferation of enterocytes enlarges the absorption surface in the rat colon and stimulates calcium and magnesium absorption. Furthermore, Tribst et al [ 27 ] reported that supplementation with probiotics (Lactobacillus acidophilus, Enterococcus faecium, Bifidobacterium thermophilum, and Bifidobacterium longum), prebiotics (mannan oligosaccharide), and synbiotics significantly increased phosphorus (P), calcium (Ca), magnesium (Mg), bone mineral density, bone mineral content, strength, resilience, and size of the area of the femoral diaphysis of rats. The ability of Bifidobacterium and Lactobacillus in colonizing the intestinal led to a decrease in the pH.…”

Prebiotics mannan-oligosaccharides accelerate sexual maturity in rats: A randomized preclinical study

“…The diets and drinking water were provided at will during the 56 days of the experimental period (Figure -1). Concealment of allocation, treatment, and animal management strategy, blinding during and after the intervention, and concealment of evaluations and analysis of results were performed to reduce bias in the study [14].…”

Efeito da suplementação de alimentos funcionais no músculo esquelético de ratos expostos cronicamente ao tabagismo

“…Alimentos funcionais, probióticos, prebióticos e simbióticos, têm demonstrado efeitos antiinflamatórios, imunomoduladores e antioxidantes (HAMED et al, 2018;ROMBI;RUFINO;BREMER-NETO, 2021;TRIBST et al, 2019;YOUNAN et al, 2019) Essa técnica, DF, permite caracterizar estruturas irregulares em lâminas histológicas, a desorganização do músculo esquelético e quantificar alterações existentes (CURY et al, 2018;OLIVEIRA et al, 2020;OZAKI et al, 2015). O objetivo desse estudo foi avaliar o efeito da suplementação na ração de alimentos funcionais, probiótico, prebiótico e simbiótico, de ratos na fase de crescimento expostos cronicamente a fumaça de cigarro, através da dimensão fractal.…”