Encapsulation of Lactobacillus acidophilus La-5 and Bifidobacterium Bb-12 by spray drying and evaluation of its resistance in simulated gastrointestinal conditions, thermal treatments and storage conditions

Abstract

Lactobacillus acidophillus La-5 (ML) and Bifidobacterium Bb-12 (MB) microparticles were produced at different temperatures by spray dryer. The influence of different temperatures on the viability, encapsulation efficiency, water activity and moisture were evaluated. Microparticles that presented more viability were submitted to thermal resistance, gastrointestinal simulation, storage stability, morphology and particle size analyses. Drying temperature of 130°C showed higher encapsulation efficiency, 84.61 and 79.73% for Lactobacillus acidophillus (ML) and Bifidobacterium Bb-12 (MB) microparticles, respectively. In the evaluation of thermal resistance and gastrointestinal simulation, the microparticles of Lactobacillus acidophillus La-5 (ML) presented higher survival than Bifidobacterium Bb-12 (MB) under these conditions. In storage viability only the Lactobacillus acidophillus La-5 (ML) microparticles remained viable at all evaluated temperatures during the 120 days. The particle sizes reported were 4.85 for Lactobacillus acidophillus La-5 (ML) and 8.75 for Bifidobacterium Bb-12 (MB), being in agreement with the desired values for products obtained by spray dryer. Finally, the Lactobacillus acidophilus La-5 (ML) microparticles were shown to be more resistant under the conditions evaluated in this study.

Microencapsulation of Probiotic Strains by Lyophilization Is Efficient in Maintaining the Viability of Microorganisms and Modulation of Fecal Microbiota in Cats

“…Ananta et al [ 19 ] showed that the higher the drying temperature of L. rhamnosus by spray drying, the lower the survival rate. Nunes et al [ 20 ] also observed a reduction in bacterial viability with temperature in the microencapsulation of B. animalis Bb-12 and L. acidophilus La-5 by spray drying (10°C to 140°C). A reduction of 2.7 log CFU/g was observed immediately after spray drying at 140°C.…”

“…Protective agents can be used to minimize this problem. Trehalose, a glucose disaccharide, is an effective thermoprotectant at low and high temperatures (Sunny-Roberts and Knorr) [ 20 ]. Studying the effects of trehalose on S. cerevisiae , Trevisol et al [ 22 ] found that this sugar increases not only the stress resistance of yeast cells but also their fermentation capacity.…”

Ricotta whey supplemented with gelatin and collagen for the encapsulation of probiotic lactic acid bacteria

“…Regardless of the ratio of encapsulating materials, LA-5 showed higher resistance to SD encapsulation than BB-12. Nunes et al (2018) also observed higher survival rates of SD-encapsulated LA-5 (84.61%) than BB-12 (79.73%), using a mixture of Arabic gum, maltodextrin, glycerol, and Tween 80. Using goat milk as encapsulating agent, Ranadheera et al (2015) also observed that LA-5 had lower loss of viability during SD than BB-12.…”

Effect of encapsulation methods and materials on the survival and viability of Lactobacillus acidophilus: A review

“…As shown in Table 3, encapsulation of Lactobacillus acidophilus by spray drying using maltodextrin as wall material did not provide a good result, but when it is combined with other materials such as dairy proteins or arabic gum the probiotic bacterial survival improves (Behboudi‐Jobbehdar et al ., 2013; Soukoulis et al ., 2013; Nunes et al ., 2018; Arepally et al ., 2020).…”