Microencapsulation of Probiotic Strains by Lyophilization Is Efficient in Maintaining the Viability of Microorganisms and Modulation of Fecal Microbiota in Cats

Rodrigues, Bruna Moura; Olivo, Paula Martins; Osmari, Milene Puntel; Vasconcellos, Ricardo Souza; Ribeiro, Leonir Bueno; Bánkuti, Ferenc Istvan; Pozza, M. S. S.

doi:10.1155/2020/1293481

Cited by 17 publications

(2 citation statements)

References 45 publications

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“…The outcomes were the same as those found by, who found that lyophilized probiotic cells improve viability without degrading any potential feature. The results were consistent with findings those of Rodrigues et al [16], which indicated that lyophilized probiotic cells increase survivability without compromising any potential feature.…”

Section: Discussionsupporting

confidence: 92%

Stability evaluation of lyophilized probiotic bacteria

Sardar¹,

Hossain²

2022

GSC Biol. Pharm. Sci.

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The unregulated use of antibiotics has emerged as a major driving force of antibiotic resistance and public health hazard, particularly in developing countries. This necessitates to find out a solution that benefits humans and animals. Probiotics are one such alternative; they are simply known as beneficial bacteria that exert health benefits to living beings. Probiotic microorganisms are crucial for the biotechnology and food industries. They are frequently employed as starters for the production of probiotic goods, food (such as yogurt, cheese and fermented meats), and food as well as for green chemistry uses. Bacteria can be conveniently preserved using lyophilization. It permits long-term storage and inexpensive distribution at suprazero temperatures while limiting viability and functionality losses by lowering water activity to values below 0.2. This study aimed to determine the efficacy of lyophilized probiotic after lyophilization. All the lyophilized isolates were found to be Gram positive and showed no catalase activity. The isolates fermented the sugars viz. D-glucose, Lactose, and Sucrose. Lyophilized bacteria showed tolerance against 2% and 4% NaCl concentrations. The bacteria also showed tolerance against 0.3% bile salt and pH 3. The isolates were able to inhibit the growth of enteric pathogens viz. Salmonella typhi, Escherichia coli, and Vibrio cholera and resistant to commercially available antibiotics viz. gentamicin, and tetracycline. It is possible to conclude that lyophilization does not affects the efficacy of probiotic bacteria. The study's findings could open up new avenues for the application of probiotic-based feeds and foods as an alternative to antibiotic among stakeholders.

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Section: Discussionsupporting

confidence: 92%

Stability evaluation of lyophilized probiotic bacteria

Sardar¹,

Hossain²

2022

GSC Biol. Pharm. Sci.

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“…Studies have determined that bacteriocin-producing Pediococcus acidilactici can relieve constipation in mice and regulate intestinal flora [ 40 ]. The microencapsulation of probiotic strains by lyophilization can maintain microbial viability in cats and improve Lactobacillus count, exerting a positive effect on cat gut microbiota [ 41 ]. The supplementation of Lactobacillus acidophilus in healthy cat diets resulted in an increase the number of Lactobacillus in their feces and a decrease in the number of E. coli , which can effectively improve the fecal quality parameters and intestinal health of healthy adult cats [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of a Multistrain Probiotic on Feline Gut Health through the Fecal Microbiota and Its Metabolite SCFAs

Ali

Lei

et al. 2023

Metabolites

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With the increasing awareness of raising pets following scientific methods, people are becoming increasingly more interested in the nutrition and health of pets, especially their intestinal health, which has become a research hotspot. Both Saccharomyces boulardii and Pediococcus acidilactici are probiotics with strong probiotic properties that can maintain the balance of intestinal flora. However, the role of Saccharomyces boulardii and Pediococcus acidilactici in felines has not been comprehensively studied to date. The aim of this study is to investigate the effect of multistrain probiotics consisting of Saccharomyces boulardii and Pediococcus acidilactici on the gut health of felines by modulating gut microbes and the production of metabolite SCFAs. The results show that the multistrain probiotic did not alter the intestinal microbial diversity and structure of short-haired domestic cats, promoted the colonization of beneficial bacteria, increased the levels of microbiota-derived SCFAs and fecal antioxidants, and reduced the levels of fecal inflammatory markers. In conclusion, the use of a multistrain probiotic in healthy, short-haired domestic cats can promote gut health by modulating gut microbes, improving microbiota-derived SCFA production, reducing inflammatory conditions, and improving antioxidant status. These results provide new insights for further exploration of the role of probiotics in the gut microbiome of cats.

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