Expression analysis of genes involved in the synthesis of oleic and linoleic acids in Jatropha cinerea seeds from Northwestern Mexico

Abstract

Plants belonging to genus Jatropha has arisen interest because of its high oil content that could be used to produce biodiesel. It is also widely reported that the main fatty acids in Jatropha oilseed are oleic and linoleic acids. However, there are scarce studies related to native species of Jatropha from Northwestern Mexico which are adapted to arid conditions, and the expression of genes involved in fatty acid synthesis for these species is still unknown. Therefore, the aim of this study was to analyze the expression of five genes, ACP1, KASII, D9SD, FAD2-1 and FAD2-2, which are involved in the oleic and linoleic acids synthesis in mature wild-seeds of Jatropha cinerea, a native species from Sonoran Desert, using semi-quantitative RT-PCR. The J. cinerea seeds were randomly collected in Bahía de Kino, Sonora (México) which is a region characterized by its harsh environments such as saline soils and extreme temperature changes and J. curcas mature seeds from a non-toxic variety from Veracruz, Mexico were used as a reference. The RT-PCR analysis of three biological replicates were considered to ensure data consistent. Our analysis showed a higher expression of KASII and FAD2-1 genes in J. cinerea seeds compared to J. curcas, meanwhile the expression of ACP1, D9SD and FAD2-2 were higher in J. curcas. Furthermore, Actin and FAD2-1 genes sequences here obtained are the first reported for J. cinerea, thus providing information to develop further studies.

Composición de los ácidos grasos de genotipos de Jatropha curcas L., en Colombia

“…Los reportes a nivel mundial coinciden y destacan el aceite de JC como una importante fuente de materia prima para la producción de biodiésel por sus características fisicoquímicas (Becker y Makkar, 2008;Wani et al, 2012;Martínez-Díaz et al, 2017;Martiñón et al, 2018). Para efectos del mejoramiento genético de esta especie en las condiciones de Colombia, el conocimiento de los genes KASII y FAD2-1, responsables de la síntesis de ácido oleico y linoleico (Lovio-Fragoso et al, 2018)…”

A GIS Approach Land Suitability and Availability Analysis of Jatropha Curcas L. Growth in Mexico as a Potential Source for Biodiesel Production

“…The number of rows and columns is defined by the number of criteria in order to be weighed by the criteria used [58,59]. This process was conducted by using the experience of the authors and based on literature review of previous experimental studies of JCL cultivation in Mexico [60][61][62][63][64][65][66][67].…”