Diagnostic methods for identification of root-knot nematodes species from Brazil

Cunha, Tiago Garcia da; Visôtto, Liliane Evangelista; Lopes, E. A.; Oliveira, C.; God, Pedro Ivo Vieira Good

doi:10.1590/0103-8478cr20170449

Cited by 29 publications

(26 citation statements)

References 35 publications

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“…In order to manage the root-knot nematodes, the ideal is to identify the species that are occurring in the area (CUNHA et al, 2018). In an area that only occurs M. enterolobii, for example, in addition to the cultivars resistant to the three species evaluated, we can recommend the planting of the cultivar Black Seed Simpson.…”

Section: Resultsmentioning

confidence: 99%

Host status of crispy-leaf lettuce cultivars to root-knot nematodes

Sgorlon¹,

Silva²,

Soares³

et al. 2018

Biosci. J.

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Lettuce is the main leafy vegetable grown in the world, being the crispy-leaf lettuce type predominant. With consecutive cultivation in the same area, several factors may impair yield, highlighting the damage caused by root-knot nematodes, Meloidogyne spp. This study aimed at evaluating the reaction of twenty crispy-leaf lettuce cultivars to Meloidogyne incognita race 3, M. javanica and M. enterolobii. Three experiments were conducted, one for each nematode species. The experiments were carried out in a greenhouse, in pots with sterilized substrate. The design was completely randomized with five replications. Seedlings were inoculated with 1.000 eggs and second-stage juveniles of nematode per pot, on the day of transplantation of seedlings. The tomato 'Rutgers' was used as inoculum viability control for each specie tested. The variables evaluated were: reproduction factor (FR), total number of eggs and second-stage juveniles (NTOJ) and number of eggs and second-stage juveniles per gram of root (NOJGR), 60 days after inoculation. The results showed that the cultivars Veronica, Grand Rapids and Crespa para Verão are resistant to the three nematode species. The cultivars Thaís, SRV 2005 and Marisa are resistant to M. incognita race 3 and M. javanica. The cultivar Black Seed Simpson is resistant to M. enterolobii. The cultivars Vanda and Mônica SF 31 are resistant to M. incognita race 3. The cultivars Crespa, Rubia, Cinderela and Veneranda are resistant to M. javanica.

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Section: Resultsmentioning

confidence: 99%

Host status of crispy-leaf lettuce cultivars to root-knot nematodes

Sgorlon¹,

Silva²,

Soares³

et al. 2018

Biosci. J.

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“…Considering that morphological and morphometrical methods for the identification species of RKNs are considerably time consuming and there is a lot of ambiguity, using species-specific primers could be a rapid method for detection (these primers listed in Cunha et al, 2018). The genetic diversity among M. javanica populations has been inferred by different markers (reviewed in Blok and Powers, 2009;Mokaram Hesar et al, 2011).…”

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confidence: 99%

Genetic intraspecific diversity of Meloidogyne javanica parasitizing vegetables in southern Iran

Ghaderi

Dehghan

Hesar

et al. 2020

Journal of Nematology

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In order to investigate different species of root-knot nematodes associated with vegetable production in southern regions of Iran, 37 populations of the most predominant species, Meloidogyne javanica, were recovered. Morphological and morphometric studies were carried on the characters of females, males, J2s and perineal patterns. Species-specific Sequence Characterized Amplified Region (SCAR) primers confirmed morphological studies, and all these populations produced specific band in 670 bp using Fjav and Rjav primers. Genetic diversity of different populations was studied by Inter Simple Sequence Repeats (ISSR) and Random Amplified Polymorphic DNA (RAPD) markers implementing 10 primers for each approach. Results revealed a relatively low genetic diversity (the percentage of polymorphic bands were 19.08 and 24.60 for ISSR and RAPD, respectively). The analyses of molecular variance indicated that the variation resulted from genotypic variations within region and variances among regions are 81% and 19% for RAPD, and 86% and 14% for ISSR, respectively. On the other hand, F ST and Nm values are 0.140 and 1.535 for ISSR while these values are 0.188 and 1.079 for RAPD. So it can be concluded that there is a great deal of gene flow between populations due to the movement of plant material contaminated with nematodes, which results in high mixing between populations. ISSR and RAPD datasets failed to group populations according to their geographic region. There were no pathotypes or other intraspecific biological entities observed in the species.

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“…The presence of the Mi gene confers resistance to M. incognita, M. javanica, M. arenaria and to insects such as aphids and whiteflies. Some species and/or populations of Meloidogyne called virulent pathotypes have the capacity to withstand high temperatures and supplant the resistance conferred by the Mi gene (CORTADA et al, 2011). In addition, expression of the Mi gene is heat sensitive and its effect can be lost when the soil temperature surpasses 28 °C, allowing the nematode to reproduce in the plant root, even if it contains the resistance gene (DROPKIN, 1969).…”

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confidence: 99%

“…Isoenzymatic of esterase (EST-I1) and malate dehydrogenase (MDH-N1) phenotypes showed patterns characteristic of M. incognita specie ( Figure 1C-D). However, for more refined phylogenetic studies a more complete diagnosis using molecular techniques is also suggested (CUNHA, et al, 2018).…”

mentioning

confidence: 99%

“…Individuals or populations of Meloidogyne spp. naturally virulent to the Mi gene may occur even without selection pressure and is not rare; several cases have already been reported in European countries (CORTADA et al, 2011). One of the hypotheses raised is that this phenomenon is caused by the selection pressure by the repeated exposure of the cultivar ported from the Mi gene to the nematode, either in the field or laboratory.…”

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confidence: 99%

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Supplanting resistance of the Mi gene by root-knot nematode in industrial tomato in the Cerrado in Goiás State of Brazil

et al. 2019

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Problems with root-knot nematodes, caused by genus Meloidogyne, can be controlled through the introduction of resistance genes in commercial varieties of tomatoes. However, plants of the hybrid 'Heinz 9992' for industrial processing, carrying the Mi gene, were reported with their roots severely infected in experimental cultivation in Goiás State of Brazil with symptoms typical of galls caused by Meloidogyne spp. After dissecting the galls present in the root samples, the females of the nematodes were removed and afterwards, performed the analysis of the perineal pattern and the biochemical characterization by electrophoresis of isoenzymes, besides the pathogenicity test. The pathogen analyzed was identified as Meloidogyne incognita. The pathogenicity test confirmed the supplanting resistance of the tomato hybrid with the Mi gene by this virulent population of M. incognita.

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Diagnostic methods for identification of root-knot nematodes species from Brazil

Cited by 29 publications

References 35 publications

Host status of crispy-leaf lettuce cultivars to root-knot nematodes

Host status of crispy-leaf lettuce cultivars to root-knot nematodes

Genetic intraspecific diversity of Meloidogyne javanica parasitizing vegetables in southern Iran

Supplanting resistance of the Mi gene by root-knot nematode in industrial tomato in the Cerrado in Goiás State of Brazil

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