Micropropagation of the new apple rootstock ‘G. 814’

Meneguzzi, Aline; Gonçalves, Mayra Juline; Camargo, Samila Silva; Grimaldi, Fernanda; Weber, Gabriela Candido; Rufato, Léo

doi:10.1590/0103-8478cr20160615

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…Stanley were sterilized and in vitro propagated on MS medium boosted with 0.5 mg/l BAP combined with 0.1 mg/l IBA, resulting in an average number of 3.08 shoots per explant and 3.33 cm average shoot length per explant (18). In a study on micropropagation of new apple rootstock G814 (40), noted that BAP at 1.0 mg/l gives finest results at mul-tiplication phase and produced a greater number of roots (2.0). On MS medium enriched with the cytokinin (0.5 and 1.0 mg/L) in conjunction with TDZ, while Principe Grande (55 %) and Barburina pear cultivars (50 %) had the highest rates of shoot initiation (17).…”

Section: In Vitro Shoot Proliferationmentioning

confidence: 99%

Micropropagation of fruit crops: a review

et al. 2022

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In vitro culture has the greatest commercial value in the extremely quick creation of clonal plants compared to conventional techniques of propagation. It has also proven to be of tremendous practical utility as assistance to the development and maintenance of disease-free planting material, germplasm conservation, and supplements to the regular methods of plant progress, such as plant genetic engineering. The discovery and implementation of current tissue culture techniques are thought to be paving the way for a second green revolution. Here, the standardization of techniques for the in vitro clonal propagation of fruit crops is highlighted. In addition, we summarize the effects of several surface sterilizing agents, plant growth regulators (PGRs), and anti-phenolic chemicals on culture establishment, shoot proliferation, in vitro rooting, hardening and economics of in vitro generation of true-to-type plants.

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Section: In Vitro Shoot Proliferationmentioning

confidence: 99%

Micropropagation of fruit crops: a review

et al. 2022

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In vitro tissue culture of apple and other Malus species: recent advances and applications

Silva

Gulyás

Magyar-Tábori

et al. 2019

Planta

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Main conclusion Studies on the tissue culture of apple have allowed for molecular, biotechnological and applied breeding research to advance. In the past 8 years, over 100 papers advancing basic biology, genetic transformation and cryobiology have emerged. Apple (Malus × domestica Borkh.; Rosaceae) is an important fruit crop grown mainly in temperate regions of the world. In vitro tissue culture is a biotechnological technique that has been used to genetically improve cultivars (scions) and rootstocks. This updated review presents a synthesis of findings related to the tissue culture of apple and other Malus spp. between 2010 and 2018. Increasingly complex molecular studies that are examining the apple genome, for example, in a bid to identify the cause of epigenetic mutations and the role of transposable elements in this process would benefit from genetically stable source material, which can be produced in vitro. Several notable or curious in vitro culture methods have been reported to improve shoot regeneration and induce the production of tetraploids in apple cultivars and rootstocks. Existing studies have revealed the molecular mechanism underlying the inhibition of adventitious roots by cytokinin. The use of the plant growth correction factor allows hypothetical shoot production from leaf-derived thin cell layers relative to conventional leaf explants to be determined. This updated review will allow novices and established researchers to advance apple and Malus biotechnology and breeding programs.

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Optimization of Hormonal Compositions of Media for In vitro Propagation of Apple (Malus ×domestica Borkh.) Cultivars

Abdella¹,

Yusuf²,

Petros³

2023

TOBIOTJ

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Background Apple (Malus × domestica Borkh.) is one of most important fruit crop in the world. Traditionally, vegetative propagation methods (including cutting, budding, and layering) are time consuming (about three years), with a low production rates and low success in obtaining virus-free plants. Objective The present study was planned to investigate the in vitro propagation of apple (M. domestica) cultivars from nodal segments. Methodology The Murashige and Skoog (MS) medium supplemented with sucrose and different concentrations of plant growth regulators (PGRs) were used for shoot proliferation and root induction. The optimal concentrations of PGRs in MS medium were assessed. The effect of full and half strength MS medium on root induction was investigated. Results Examination of the effects of MS medium supplemented with various concentrations of indole-3-acetic acid (IAA) and kinetin revealed that significantly highest shoot response was recorded for the ‘Princess’ cultivar with maximum shoot proliferation rate (65.25%), shoot number per explant (2.57), shoot length (7.28cm), and leaf number per shoot (6.15) after four weeks of culture. The root induction in microshoots of three apple cultivars was observed after 20 days of culturing. The strength MS medium (full and half) containing 1.5 mg/L IAA significantly affected (at P<0.05, chi square test) root induction in all three apple cultivars, especially rooting rate. However, there was no significant difference in root number and root length per microshoot among the apple cultivars. Among the cultivars, significantly highest rooting rate(48.30%), root number (6.25),and root length (4.15cm)were recorded for cultivar ‘Princess’ on full strength MS medium. Conclusion PGR combination of IAA (1.0 mg/L) and kinetin (3.0 mg/L) was found to be the best for shoot proliferations. The shoot responses were found to increase with an increase in kinetin concentration combined with IAA at 1.00 mg/L.

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Micropropagation of the new apple rootstock ‘G. 814’

Cited by 3 publications

References 11 publications

Micropropagation of fruit crops: a review

Micropropagation of fruit crops: a review

In vitro tissue culture of apple and other Malus species: recent advances and applications

Optimization of Hormonal Compositions of Media for In vitro Propagation of Apple (Malus ×domestica Borkh.) Cultivars

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