Applications of 5-aminolevulinic acid on the physiological and biochemical characteristics of strawberry fruit during postharvest cold storage

Li, Yi; Liu, Zhiqiang; Wang, Liangju

doi:10.1590/0103-8478cr20151426

Cited by 11 publications

(6 citation statements)

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“…These results suggest that ALA-induced structural gene expression partially depends on MdMYB10 and MdMYB9 . In fact, MYB9 group is a rare type of anthocyanin-related MYB activator, which is lost in most dicot species ( Zhou et al, 2016 ), whereas ALA is able to activate anthocyanin biosynthesis in various fruit species ( Watanabe et al, 2006 ; Xiao et al, 2012 ; Guo et al, 2013 ; Feng et al, 2015 ; Li et al, 2016 ; Ye et al, 2017 ), suggesting that ALA-induced accumulation of anthocyanin is coordinately regulated by a set of R2R3-MYB genes. Moreover, among the DEGs of TFs, we also identified bHLH , AP2-EREBP , WRKY , bZIP , and NAC family members ( Supplementary Table 9 ).…”

Section: Discussionmentioning

confidence: 99%

“…5-Aminolevulinic acid (ALA), a key precursor in tetrapyrrole biosynthesis, has drawn increasing attention due to its important regulation roles in multiple physiological processes, including plant development, secondary metabolism, and fruit ripening ( Akram and Ashraf, 2013 ). Promoting red fruit coloration is one of the most outstanding roles of ALA, which has been widely demonstrated in apple ( Wang et al, 2006 ; Xie et al, 2013 ; Zheng et al, 2017 ), grape ( Watanabe et al, 2006 ), pear ( Xiao et al, 2012 ), peach ( Guo et al, 2013 ; Ye et al, 2017 ), litchi ( Feng et al, 2015 ), and strawberry ( Li et al, 2016 ), indicating great application potential of ALA in modern fruit production. However, the molecular mechanism underlying ALA-induced fruit coloration is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic Profiling of Apple Calli With a Focus on the Key Genes for ALA-Induced Anthocyanin Accumulation

et al. 2021

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The red color is an attractive trait of fruit and determines its market acceptance. 5-Aminolevulinic acid (ALA), an eco-friendly plant growth regulator, has played a universal role in plant secondary metabolism regulation, particularly in flavonoid biosynthesis. It has been widely reported that ALA can up-regulate expression levels of several structural genes related to flavonoid metabolism and anthocyanin accumulation. However, the molecular mechanisms behind ALA-induced expression of these genes are complicated and still far from being completely understood. In this study, transcriptome analysis identified the differentially expressed genes (DEGs) associated with ALA-induced anthocyanin accumulation. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the flavonoid biosynthesis (ko00941) pathway was significantly enhanced in the ALA-treated apple calli at 24, 48, and 72 h after the treatment. Expression pattern revealed that ALA up-regulated the expression of the structural genes related to not only anthocyanin biosynthesis (MdCHS, MdCHI, MdF3’H, MdDFR, MdANS, and MdUFGT) but also anthocyanin transport (MdGST and MdMATE). Two R2R3-MYB transcription factors (MdMYB10 and MdMYB9), which are the known positive regulators of anthocyanin biosynthesis, were significantly induced by ALA. Gene overexpression and RNA interference assays demonstrated that MdMYB10 and MdMYB9 were involved in ALA-induced anthocyanin biosynthesis. Moreover, MdMYB10 and MdMYB9 might positively regulate the transcription of MdMATE8 by binding to the promoter region. These results indicate that MdMYB10 and MdMYB9 modulated structural gene expression of anthocyanin biosynthesis and transport in response to ALA-mediated apple calli coloration at the transcript level. We herein provide new details regarding transcriptional regulation of ALA-induced color development.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptomic Profiling of Apple Calli With a Focus on the Key Genes for ALA-Induced Anthocyanin Accumulation

et al. 2021

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“…After cooling, the samples were centrifuged at 2500 rpm for 10 minutes. The light absorption of the supernatant was measured at 532 nm . Light absorbance indicates the MDA content.…”

Section: Methodsmentioning

confidence: 99%

Study on a 65‐mer peptide mimetic enzyme with GPx and SOD dual function

Yin

Zhuang

et al. 2018

J of Molecular Recognition

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Excessive reactive oxygen species (ROS) levels are harmful to the body. The peroxidase, GPx, and the superoxide dismutase, SOD, are important antioxidant enzymes for preventing ROS-induced damage. Se-CuZn-65P is an enzyme mimetic with dual GPx and SOD antioxidant function. However, currently, its production is mainly based on the cysteine auxotrophic expression technique, which is inefficient, expensive, and time consuming. In this study, we combined protein engineering and the chemical mutation method to synthesize Se-CuZn-65P. The DNA sequence encoding the 65 amino acid peptide with the desired sequence transformations to incorporate the SOD and the GPx catalytic sites was cloned and expressed in a soluble protein expression vector. The protein yield increased up to 152 mg/L, which is 10 times higher than in previous studies. The SOD and GPx activity of Se-CuZn-65P was high (1181 U/mg and 753 U/μmol, respectively). The binding constant of glutathione was 5.6 × 10 L·mol , which shows that Se-CuZn-65P efficiently catalyzed hydrogen peroxide reduction by glutathione. Mitochondrial damage experiments confirmed the double protective role of the Se-CuZn-65P peptide and demonstrated functional synergy between the SOD and the GPx domains, which indicates its potential to be used in the treatment of ROS-related diseases. Our research may give a new thought to increase the yield of mimic.

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“…5‐Aminolevulinic acid (5‐ALA) is a non‐proteinogenic amino acid existing in most living organisms as a metabolic intermediate toward biosynthesis of essential tetrapyrrole/porphyrin pigment compounds, such as heme (Schlicke et al, 2015; Figure 1). Practically, 5‐ALA has broad applications in many fields, such as medicine (Inoue, 2017; Juzeniene, Juzenas, Iani, & Moan, 2002), agriculture (Hotta, Tanaka, Takaoka, Takeuchi, & Konnai, 1997), and food preservation (Y. Li, Li, & Wang, 2016). In nature, there are two major metabolic routes for 5‐ALA biosynthesis: the C4 (or Shemin) pathway and the C5 pathway.…”

Section: Introductionmentioning

confidence: 99%

Strain engineering for high‐level 5‐aminolevulinic acid production in Escherichia coli

Miscevic

Mao

Kefale

et al. 2020

Biotech & Bioengineering

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Herein, we report the development of a microbial bioprocess for high-level production of 5-aminolevulinic acid (5-ALA), a valuable non-proteinogenic amino acid with multiple applications in medical, agricultural, and food industries, using Escherichia coli as a cell factory. We first implemented the Shemin (i.e., C4) pathway for heterologous 5-ALA biosynthesis in E. coli. To reduce, but not to abolish, the carbon flux toward essential tetrapyrrole/porphyrin biosynthesis, we applied clustered regularly interspersed short palindromic repeats interference (CRISPRi) to repress hemB expression, leading to extracellular 5-ALA accumulation. We then applied metabolic engineering strategies to direct more dissimilated carbon flux toward the key precursor of succinyl-CoA for enhanced 5-ALA biosynthesis. Using these engineered E. coli strains for bioreactor cultivation, we successfully demonstrated high-level 5-ALA biosynthesis from glycerol (~30 g L −1) under both microaerobic and aerobic conditions, achieving up to 5.95 g L −1 (36.9% of the theoretical maximum yield) and 6.93 g L −1 (50.9% of the theoretical maximum yield) 5-ALA, respectively. This study represents one of the most effective bio-based production of 5-ALA from a structurally unrelated carbon to date, highlighting the importance of integrated strain engineering and bioprocessing strategies to enhance bio-based production.

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Applications of 5-aminolevulinic acid on the physiological and biochemical characteristics of strawberry fruit during postharvest cold storage

Abstract: The compound 5-aminolevulinic acid (ALA) is a key precursor in the biosynthesis

Cited by 11 publications

References 14 publications

Transcriptomic Profiling of Apple Calli With a Focus on the Key Genes for ALA-Induced Anthocyanin Accumulation

Transcriptomic Profiling of Apple Calli With a Focus on the Key Genes for ALA-Induced Anthocyanin Accumulation

Study on a 65‐mer peptide mimetic enzyme with GPx and SOD dual function

Strain engineering for high‐level 5‐aminolevulinic acid production in Escherichia coli

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