An approach on phytoalexins: function, characterization and biosynthesis in plants of the family Poaceae

Arruda, Rejanne Lima; Paz, Andressa Tuane Santana; Bara, Maria Teresa Freitas; Cortes, Marcio Vinicius de Carvalho Barros; Filippi, Marta Cristina Corsi de; Conceição, Edemilson Cardoso da

doi:10.1590/0103-8478cr20151164

Cited by 23 publications

(13 citation statements)

References 55 publications

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“…Phytoalexins are defined as “any low-molecular-weight, anti-microbial secondary metabolites that are synthesized and accumulate in plants in response to biotic and abiotic stress” (Ahuja et al, 2012). Phytoalexins have been characterized amongst different classes of chemical compounds such as coumarins, terpenoids, flavonoid, alkaloids, stilbenes, phenolic compounds, and many others (Arruda et al, 2016). According to our pathway enrichment analysis, the indole alkaloid biosynthesis, flavonoid biosynthesis, and triterpene biosynthesis pathways exhibited more up-regulated genes in resistant lines than in susceptible lines.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis between Broccoli (Brassica oleracea var. italica) and Wild Cabbage (Brassica macrocarpa Guss.) in Response to Plasmodiophora brassicae during Different Infection Stages

et al. 2016

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Clubroot, one of the most devastating diseases to the Brassicaceae family, is caused by the obligate biotrophic pathogen Plasmodiophora brassicae. However, studies of the molecular basis of disease resistance are still poor especially in quantitative resistance. In the present paper, two previously identified genotypes, a clubroot-resistant genotype (wild cabbage, B2013) and a clubroot-susceptible genotype (broccoli, 90196) were inoculated by P. brassicae for 0 (T0), 7 (T7), and 14 (T14) day after inoculation (DAI). Gene expression pattern analysis suggested that response changes in transcript level of two genotypes under P. brassicae infection were mainly activated at the primary stage (T7). Based on the results of DEGs functional enrichments from two infection stages, genes associated with cell wall biosynthesis, glucosinolate biosynthesis, and plant hormone signal transduction showed down-regulated at T14 compared to T7, indicating that defense responses to P. brassicae were induced earlier, and related pathways were repressed at T14. In addition, the genes related to NBS-LRR proteins, SA signal transduction, cell wall and phytoalexins biosynthesis, chitinase, Ca2+ signals and RBOH proteins were mainly up-regulated in B2013 by comparing those of 90196, indicating the pathways of response defense to clubroot were activated in the resistant genotype. This is the first report about comparative transcriptome analysis for broccoli and its wild relative during the different stages of P. brassicae infection and the results should be useful for molecular assisted screening and breeding of clubroot-resistant genotypes.

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Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis between Broccoli (Brassica oleracea var. italica) and Wild Cabbage (Brassica macrocarpa Guss.) in Response to Plasmodiophora brassicae during Different Infection Stages

et al. 2016

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“…Medicinal plants are broadly used to cure various infections and also used as a precursor for the synthesis of natural drugs. Bioactive compounds responsible for therapeutic effects of plants are normally stored in plant cells as secondary metabolites and may vary in concentration depending on plant part, season, climate and growth phase [9].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Phytochemical Constituents and Antibacterial Activity of Chromolaena Odorata L. Leaf Extract against Selected Multidrug Resistant Bacteria Isolated from Wounds

Alabi

Olusola-Makinde

Oladunmoye

2020

SAJRM

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Aim: To study the antibacterial effect of Chromolaena odorata extracts on multidrug resistant Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae from wounds. Place of Study: University of Medical Sciences Teaching Hospital, Akure, Ondo State, Nigeria, between January and June 2019. Methodology: A total of 87 wound swabs were collected from patients of University of Medical Sciences Teaching Hospital, Akure. Air-dried and powdered C. odorata leaves were extracted using hot water, ethanol and methanol as extraction solvents and concentrated using a rotary evaporator. The concentrated C. odorata extracts were purified using chromatographic techniques. Qualitative and quantitative phytochemical screening of C. odorata extracts were done by standard methods. Antibiotics susceptibility pattern of bacterial isolates to a panel of ten (10) conventional antibiotics was determined by disc diffusion method. Results: C. odorata methanolic extract had the highest extract yield (26.2%). From the multidrug resistance analysis, 66.7% of bacterial isolates tested had multidrug resistance index (MDRI) of 100%. Pseudomonas aeruginosa was susceptible to 100 mg/ml of C. odorata ethanolic extract but resistant (0.00±0.00) to 100 mg/ml of hot aqueous C. odorata extract. Conclusion: This study reveals the inhibitory activities of C. odorata extracts on multidrug resistant bacteria isolated from wounds and an indication of their potential in the treatment of wound infection.

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“…Ot is worth mentioning that, unlike other cereals, rice is the least contaminated one by Fusarium genera species in the field (Arruda et al, 2016;Dors et al, 2013). Rice bran is also a good source of γ-oryzanol, a mix of lipid and ferulic acid that has been applied for replacing synthetic antioxidant in pharmaceuticals and food chain.…”

Section: Introductionmentioning

confidence: 99%

“…Regarding this issue Heidtmann-Bemvenuti et al (2016) showed that γ-oryzanol is not only able to inhibit Fusarium development but also prevent DDN production by toxigenic species. Therefore, estimating quantitative aspects (minimal concentration to inhibit pathogens and the mycotoxins production) before proposing new strategies to prevent damage are fundamental, so as to promote sustainability and food safety (Arruda et al, 2016). The inhibition on free radical produced by chemical or enzymatic process, carry out by peroxidase, has been related to mycotins production by pathogenic fungal species (Ferrochio et al, 2013;Heidtmann-Bemvenuti et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Efficiency of γ-oryzanol against the complex Fusarium graminearum growth and mycotoxins production

2019

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Considering the challenge related to properties of γ-oryzanol as antifungal and antioxidant activities, or both, this study assessed the effect of γ-oryzanol abiltity to inhibit the complex F. graminearum CQ244 development, mycotoxin production and on oxidative process. The aim was to the viability of a new strategy to replace synthetic fungicide application in food chain. 2 Materials and methods 2.1 Raw materials and analytical standards The γ-oryzanol was obtained from rice bran donated by a company in the southern region in Rio Grande do Sul state, Brazil. The fungus complex F.graminearumCQ244, was granted from collection of Federal University of Rio Grande do Sul (UFRGS). Ot was isolated from barley, and its potential of mycotoxins production was evaluated (Astolfi et al., 2010). Mycotoxin (NOV, DDN, and ZEA) and fungicide (azoxystrobin and trifloxystrobin) standards were purchased from Sigma-Aldrich, USA (purity> 98%). The γ-oryzanol standard was obtained from Wako Chemicals, USA (purity = 98%). 2.2 Extraction and characterization of γ-oryzanol from rice bran Rice bran was evaluated about pH, acidez, percentual of moisture, ash, protein, lipids, fiber and carbohydrates by the Association of Dfficial Analytical Chemists (2000) procedures. γ-oryzanol was extracted in according to the protocol described

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An approach on phytoalexins: function, characterization and biosynthesis in plants of the family Poaceae

Abstract: Phytoalexins are compounds that have been studied a few years ago, which present mainly antimicrobial activity. The plants of the family

Cited by 23 publications

References 55 publications

Comparative Transcriptome Analysis between Broccoli (Brassica oleracea var. italica) and Wild Cabbage (Brassica macrocarpa Guss.) in Response to Plasmodiophora brassicae during Different Infection Stages

Comparative Transcriptome Analysis between Broccoli (Brassica oleracea var. italica) and Wild Cabbage (Brassica macrocarpa Guss.) in Response to Plasmodiophora brassicae during Different Infection Stages

Evaluation of Phytochemical Constituents and Antibacterial Activity of Chromolaena Odorata L. Leaf Extract against Selected Multidrug Resistant Bacteria Isolated from Wounds

Efficiency of γ-oryzanol against the complex Fusarium graminearum growth and mycotoxins production

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