Alterations in antioxidant metabolism in coffee leaves infected by Cercospora coffeicola

Abstract

Brown eye spot (BE) caused by Cercospora coffeicola is the main disease of coffee crop. A variation in symptoms of BE has been reported in the field, raising suspicion of occurrence of new species. However, information about coffee- C. coffeicola interaction is still limited. This research aimed to determine the difference between antioxidant metabolism of coffee plants cultivar Mundo Novo inoculated with a strain isolated from a common BE lesion (CML 2984) and a strain isolated from a black BE lesion (CML 2985). The enzyme activity of peroxidase (POX), catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX) and phenylalanine ammonia lyase (PAL) were determined. Activities of POX, APX, and PAL increased in plants inoculated with both strains compared to non-inoculated plants at 12 and 24 hours post inoculation (hpi). CAT activity increased in inoculated plants with black BE strain at 24 hpi and both strains at 48 hpi. The SOD activity only increased in inoculated plants with both strains at 48 hpi. These results show that an elevated antioxidant response was observed when the plants were challenged with both strains of C. coffeicola. Both strains produced lesions of the common type, suggesting that other factors lead to the development of black BE lesion type under field conditions and further investigation is needed.

Identification of mung bean germplasm for resistance against Cercospora canescens and to study the association of biochemical parameters with defense mechanisms

“…This suggests a relevant role of these enzymes in the defense metabolism of the host plant. Andrade et al (2016) also have reported a similar finding in coffee (Coffea arabica L.) crops infected with C. caffeicola, where the activity of PAL, POX, and SOD was subsequently increased at 24 and 48 hai. This depicts an elevated antioxidant activity because of invasion and subsequent infection by the hemibiotrophic pathogen.…”

Efficiency of Fungicide Application an Using an Unmanned Aerial Vehicle and Pneumatic Sprayer for Control of Hemileia vastatrix and Cercospora coffeicola in Mountain Coffee Crops

“…The methods commonly used for plant health protection in coffee crops include the use of backpack sprayers, atomizers, and hydro-pneumatic and pneumatic sprayers. The limitations of these options are scarcity of specialized workers, limited operational capacity, low application efficiency, and excessive application rates; these combined factors result in losses of product to the soil, and environmental and human contaminations [12,13].…”

Quality and sensory acceptance of ‘Pérola’ pineapple grown in soil with application of organic fertilizer

“…Type and doses of fertilizer applied and the timing of application influence the internal and external quality of pineapple fruits (COELHO et al, 2007;GUARÇONI & VENTURA, 2011;MARQUES et al, 2011, OLIVEIRA et al, 2015. According to COELHO et al (2007), the average fruit weight of the 'Jupi' pineapple was significantly higher at a dose of 93 g of NPK (20:05:20) plant -1 while the use of higher doses increased the contents of soluble solids and titratable acidity and reduced the soluble solids/ titratable acidity ratio. With respect to the timing of application, MARQUES et al (2011) observed that the application of a total nitrogen dose of 30 g plant -1 after floral induction of the 'Smooth Cayenne' pineapple produced smaller fruits, with lower ripening index and higher titratable acidity.…”