Detection of resistance genes and evaluation of water quality at zoo lakes in Brazil

Faria, Ana Carolina Silva de; Godoy, Isabela de; Sanches, Anderson Aparecido Amorim; Iglesias, G.A.; Candido, Stefhano Luiz; Paz, Regina Celia Rodrigues da; Nakazato, Luciano; Dutra, Valéria

doi:10.1590/0103-8478cr20150827

Cited by 7 publications

(5 citation statements)

References 22 publications

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“…Increase in abundance of ARGs along with changes in bacterial community composition and subsequent enrichment of any particular bacterial group have been widely reported. For example Enterobacteriaceae increase was positively correlated with ARGs in lakes in Brazilian Zoo [52]. Also during high throughput profiling of ARGs in East Tiaoxi river, role of bacterial community was found to be more effective as compared to mobile genetic elements in influencing the resistome [53].…”

Section: Role and Impact Of Community Dynamics And Mges In Args DImentioning

confidence: 99%

Contributions and Challenges of High Throughput qPCR for Determining Antimicrobial Resistance in the Environment: A Critical Review

Waseem

Jameel²,

Ali

et al. 2019

Molecules

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Expansion in whole genome sequencing and subsequent increase in antibiotic resistance targets have paved the way of high throughput qPCR (HT-qPCR) for analyzing hundreds of antimicrobial resistance genes (ARGs) in a single run. A meta-analysis of 51 selected studies is performed to evaluate ARGs abundance trends over the last 7 years. WaferGenTM SmartChip is found to be the most widely used HT-qPCR platform among others for evaluating ARGs. Up till now around 1000 environmental samples (excluding biological replicates) from different parts of the world have been analyzed on HT-qPCR. Calculated detection frequency and normalized ARGs abundance (ARGs/16S rRNA gene) reported in gut microbiome studies have shown a trend of low ARGs as compared to other environmental matrices. Disparities in the HT-qPCR data analysis which are causing difficulties to researchers in precise interpretation of results have been highlighted and a possible way forward for resolving them is also suggested. The potential of other amplification technologies and point of care or field deployable devices for analyzing ARGs have also been discussed in the review. Our review has focused on updated information regarding the role, current status and future perspectives of HT-qPCR in the field of antimicrobial resistance.

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Section: Role and Impact Of Community Dynamics And Mges In Args DImentioning

confidence: 99%

Contributions and Challenges of High Throughput qPCR for Determining Antimicrobial Resistance in the Environment: A Critical Review

Waseem

Jameel²,

Ali

et al. 2019

Molecules

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“…enrichment of a population (clonal amplification of ARB), (ii) advantageous mutations or gene rearrangements and duplication, and (iii) horizontal transfer of ecologically important genes, such as ARGs(Kondrashov 2012;Oh et al 2013).Increases in ARG abundances are related to an increased number of possible ARBs in the environment; this phenomenon was observed for the ARG abundance and number of Enterobacteriaceae in lakes in Brazilian zoos(de Faria et al 2016) and…”

mentioning

confidence: 91%

Antibiotics and antibiotic resistance genes in global lakes: A review and meta-analysis

Yang

Song

Lin

et al. 2018

Environment International

481

110

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Lakes are an important source of freshwater, containing nearly 90% of the liquid surface fresh water worldwide. Long retention times in lakes mean pollutants from discharges slowly circulate around the lakes and may lead to high ecological risk for ecosystem and human health. In recent decades, antibiotics and antibiotic resistance genes (ARGs) have been regarded as emerging pollutants. The occurrence and distribution of antibiotics and ARGs in global freshwater lakes are summarized to show the pollution level of antibiotics and ARGs and to identify some of the potential risks to ecosystem and human health. Fifty-seven antibiotics were reported at least once in the studied lakes. Our meta-analysis shows that sulfamethoxazole, sulfamerazine, sulfameter, tetracycline, oxytetracycline, erythromycin, and roxithromycin were found at high concentrations in both lake water and lake sediment. There is no significant difference in the concentration of sulfonamides in lake water from China and that from other countries worldwide; however, there was a significant difference in quinolones. Erythromycin had the lowest predicted hazardous concentration for 5% of the species (HC) and the highest ecological risk in lakes. There was no significant difference in the concentration of sulfonamide resistance genes (sul1 and sul2) in lake water and river water. There is surprisingly limited research on the role of aquatic biota in propagation of ARGs in freshwater lakes. As an environment that is susceptible to cumulative build-up of pollutants, lakes provide an important environment to study the fate of antibiotics and transport of ARGs with a broad range of niches including bacterial community, aquatic plants and animals.

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“…E. coli resistant to amoxicillin, norfloxacin, ciprofloxacin, doxycycline, and sulfamethoxazole; strains producing β-lactamase with extended spectrum (ESBL) were also found among isolates from the Belém and Barigui rivers in Paraná [98]. A positive correlation between the number of Enterobacteriaceae isolates and the resistance genes abundancy was observed by Faria et al [107] in a zoo lake in Cuiabá, indicating that urban sewage or another wastewater source was related to an increase in the amount of antibiotic-resistance genes in the environment.…”

Section: Aeromonasmentioning

confidence: 76%

Occurrence of Pharmaceuticals and Endocrine Disrupting Compounds in Brazilian Water and the Risks They May Represent to Human Health

Aquino

Brandt

Bottrel

et al. 2021

IJERPH

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The risks of pharmaceuticals and endocrine disrupting compounds (P&EDC) to the environment and human health are a current topic of interest. Hundreds of P&EDC may reach the environment, hence, there is a need to rank the level of concern of human exposure to these compounds. Thus, this work aimed at setting a priority list of P&EDC in Brazil, by studying their occurrence in raw and drinking water, calculating health guideline values (GV), and estimating the risks of population exposure to water intake. Data on the Brazilian pharmaceutical market as well as published data of the monitoring of Brazilian natural and drinking water have been collected by means of an exhaustive literature review. Furthermore, many foreign data were also collected to enable a comparison of the values found in Brazilian studies. A list of 55 P&EDC that have the potential to be found in Brazilian water is proposed, and for 41 of these a risk assessment was performed by estimating their margin of exposure (ME), by considering their occurrence in drinking water, and guideline values estimated from reported acceptable daily intake (ADI) data. For seven compounds the risk was deemed high (three estrogens and four anti-inflammatories), whereas for another seven compounds, it was regarded as an ‘alert’ situation. Although such risk analysis is conservative, since it has been calculated based on the highest reported P&EDC concentration in drinking water, it highlights the need to enhance their monitoring in Brazil to strengthen the database and support decision makers. An analysis of the occurrence of antimicrobial resistance agents (antibiotics, resistant bacteria, and resistance genes) in surface waters was also carried out and confirmed that such agents are present in water sources throughout Brazil, which deserves the attention of policy makers and health agents to prevent dissemination of antimicrobial resistance through water use.

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Detection of resistance genes and evaluation of water quality at zoo lakes in Brazil

Abstract: The investigation of the presence of antibioticresistance genes in aquatic environments is important to identify possible reservoirs of resistant microorganisms that could be a threat to human and animal health. The aims of this

Cited by 7 publications

References 22 publications

Contributions and Challenges of High Throughput qPCR for Determining Antimicrobial Resistance in the Environment: A Critical Review

Contributions and Challenges of High Throughput qPCR for Determining Antimicrobial Resistance in the Environment: A Critical Review

Antibiotics and antibiotic resistance genes in global lakes: A review and meta-analysis

Occurrence of Pharmaceuticals and Endocrine Disrupting Compounds in Brazilian Water and the Risks They May Represent to Human Health

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