Diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple plantlets during acclimatization

Silva, Aurilena de Aviz; Carvalho, Almy Júnior Cordeiro de; Freitas, Flávia Paiva de; Pessanha, Patrícia Gomes de Oliveira; Santos, Paulo Cesar dos; Silva, Mírian Peixoto Soares da; Vasconcelos, T.; Olivares, Fábio Lopes

doi:10.1590/0103-8478cr20141338

Cited by 4 publications

(3 citation statements)

References 12 publications

(20 reference statements)

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“…Inoculation with bacteria led to greater dry mass and root numbers in acclimatized bromeliad cv. Vitória plants (Silva et al, 2016), which was observed for some strains in the present study (Fig. 2).…”

Section: Resultssupporting

confidence: 82%

Diazotrophic bacteria in the growth of micropropagated ornamental pineapple

Silva

Florentino

Pereira

et al. 2019

Rev. Colomb. Cienc. Hortic.

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Ornamental pineapple is a hardy plant with significant landscaping value. Tissue culture of plants is viable for producing plants with a high phytosanitary quality. However, one of the difficulties with this cultivar is the acclimatization process, which is slow and can cause losses. The objective of the present study was to verify the potential of inoculation with diazotrophic bacteria for in vitro and ex vivo growth of ornamental pineapple. A group of diazotrophic bacterial strains selected at the Universidade José do Rosário Vellano (UNIFENAS) was prioritized in this study, and the treatments included bacterial strains UNIFENAS (100-13, 100-60, 100-68, 100-153, 100-167 and 100-198). These strains were evaluated in terms of their capacity to produce indole 3-acetic acid. Subsequently, plants were cultivated in a medium composed of MS medium salts (1/4), adding 1 mL of the bacterial strain. In the control treatment, the plants were maintained in 2 mL of MS medium. 7 days after inoculation, the plants were transplanted into the MS, where they were maintained for 30 days. After in vitro cultivation, the plants were transferred to pots containing commercial Plantmax® substrate and maintained under these conditions for 60 days. The diazotrophic bacteria were able to synthesize auxins, and their inoculation promoted greater growth in vitro and ex vitro in the plants. In the acclimatization phase, the plants inoculated with UNIFENAS strains (100-60, 100-68 and 100-153) promoted a higher shoot growth, chlorophyll content and nitrate reductase enzyme activity.

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Section: Resultssupporting

confidence: 82%

Diazotrophic bacteria in the growth of micropropagated ornamental pineapple

Silva

Florentino

Pereira

et al. 2019

Rev. Colomb. Cienc. Hortic.

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“…The dry mass of shoots as a function of N doses showed a quadratic response, reaching higher value in crowns treated with 12.88 g urea pot -1 (Table 3). Nitrogen fertilization tends to accelerate growth, reducing the nursery period for pineapple plantlet, as verified by Silva et al (2016) in micropropagated plantlet in the acclimatization phase and by Coelho et al (2007b) in stem section plantlet.…”

Section: Resultsmentioning

confidence: 89%

Production of shoots from ‘Smooth Cayenne’ pineapple crowns with nitrogen fertilization

et al. 2020

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Abstract Pineapple fruit crowns are rarely used as planting material in Brazil. However, they can be used to produce plantlet in situations of scarcity of planting material of adequate quality. The aim of this work was to evaluate the production and nutritional status of early shoots of different sizes produced from ‘Smooth Cayenne’ pineapple crowns with nitrogen fertilization after the removal of its apical meristem. The experimental design adopted was that of randomized blocks in a 4x4 factorial scheme, with four nitrogen doses (5, 10, 15 and 20 g of urea per pot), four minimum shoot lengths at harvesting (10, 15, 20 and 25 cm), with four replicates. Under full sun cultivation conditions, crowns produced 18.11 and 6.83 shoots with 10 and 25 cm in length, respectively, up to 380 days after planting and removal of the apical meristem. Pineapple shoots harvested at 180 days presented better biometric quality and can be harvested up to 380 days after crown planting. Increasing urea doses reduce P, K and Ca levels in the dry mass of shoots.

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“…Pineapple development is dependent on nutritional status, and when plantlets are cultivated under elemental deficiencies, their growth rate and development can be reduced and may even interfere in later developmental stages (Silva et al, 2016). However, little is known about adequate nutrient content during the pineapple acclimatization phase.…”

Section: Variation Causes F Valuesmentioning

confidence: 99%

Humic acids and brassinosteroid application effects on pineapple plantlet growth and nutrition during the aclimatization phase

Santos¹,

Carvalho²,

Silva³

et al. 2018

Afr. J. Agric. Res.

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Humic acid and brassinosteroid applications may be an alternative to decrease the pineapple plantlet acclimatization in in vitro cultivation, since promising results have been observed when these substances were independently applied in other propagation methods. In this sense, the aim of the present study was to evaluate the effects of humic acids and brassinosteroid application on 'BRS Vitória' pineapple plantlets grown from in vitro cultivation during acclimatization. A randomized block design was used in a 5x2x4 factorial scheme, at five brassinosteroid doses (0, 0.25, 0.50, 0.75, 1.0 mg L-1) in the presence and absence of humic acids during four sampling periods (60, 90, 120 and 150 days after transplanting), with five replicates for each treatment. BIOBRÁS-16 was used as the brassinosteroid source, and the organic soil conditioner Agrolmin HF ® was used as the humic acid source. Plantlets were collected for evaluation every 30 days from 60 days after transplanting. The number of plantlet leaves, length and root mass were higher in the humic acid treatment without brassinosteroid application. Leaf, nitrogen, phosphorus, potassium, calcium and magnesium contents were of 13.04, 1.77, 40.2, 8.79 and 3.17 mg kg-1 , respectively. Nitrogen and potassium contents in the plantlets decreased, while phosphorus contents increased as a function of acclimatization time, regardless of treatment.

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Diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple plantlets during acclimatization

Abstract: This study examines the effect of inoculation with diazotrophic bacteria and nitrogen fertilization on the growth (0, 2, 5, 7, and 10g L -1 ), and five evaluation periods (30, 60, 90, 120, and 150

Cited by 4 publications

References 12 publications

Diazotrophic bacteria in the growth of micropropagated ornamental pineapple

Diazotrophic bacteria in the growth of micropropagated ornamental pineapple

Production of shoots from ‘Smooth Cayenne’ pineapple crowns with nitrogen fertilization

Humic acids and brassinosteroid application effects on pineapple plantlet growth and nutrition during the aclimatization phase

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