In vitro establishment of blackberry (Rubus sp.) cultivar 'Xavante'

Pelizza, Tânia Regina; Silveira, Fabiane Nunes; Ribeiro, Roberta Sabatino; Machado, Bruno Dalazen; Rufatto, Leo; Kretzschmar, Aike Anneliese

doi:10.1590/0103-8478cr20140988

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…The accentuated decline after the third (60 days) and fourth (120 days) subculture in the in vitro production of etiolated plants was mainly due to the loss of many shoots which at the start of the subculturing appeared to be healthy, but that passed through an intense oxidation process that caused the death of a significant portion of the shoots produced. A process similar to oxidation was observed in the 'Xavante' variety of blackberry, which might have been caused by the interaction of the time spent by the parent plants in the absence of light with the reduction of MS salts in the culture medium (Pelizza et al, 2016). However, many researchers have found the absence of light to be a strategy to reduce oxidation processes during in vitro cultivation, revealing the need for additional studies to understand the causes that led to such high oxidation in this experiment.…”

Section: Strategies For Vegetative Propagationmentioning

confidence: 77%

Strategies for vegetative propagation and viral cleaning of a miniature ornamental pineapple hybrid

Silva

Souza

Oliveira

et al. 2021

Acta Sci. Biol. Sci.

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This study assessed and compared different methods for vegetative propagation of a miniature ornamental pineapple hybrid (ORN-MUT), seeking to determine the best method for production of plantlets, as well as for removal of the PMWaV viral complex from plants cultured in vitro, for production of healthy parent plants. Pineapple wilt is a disease that can cause large economic and is caused by a viral complex called Pineapple mealybug wilt-associated virus (PMWaV). For this, four propagation methods were evaluated (conventional, stem sectioning, micropropagation and etiolation of nodal segments). The time necessary for each method and the number of plants formed were assessed. Stem tips (0.5 mm) were cultured and indexed for three PMWaV types. Conventional propagation produced 17 plantlets per plant in 566 days, stem sectioning produced 2.3 plantlets per stem in 591 days, while the conventional micropropagation technique produced 1,284 plants after four subcultures in 778 days. Stems etiolated for 60 days showed peak production in the second subculture, with 1,224 plants. This method required 883 days to obtain plants with ideal size for transplantation to the field. In turn, stems etiolated for 120 days produced 935 plants at the end of four subcultures, with peak output in the third subculture, in which the plants could be cultivated in the field after 943 days. Conventional micropropagation and etiolation for 60 days were the best methods for production of plantlets of the ORN-MUT hybrid. The results of this work showed that the cultivation of shoot tips is an efficient strategy to remove the PMWaV complex and obtain healthy mother plants and can be a useful tool for other varieties of pineapple.

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Section: Strategies For Vegetative Propagationmentioning

confidence: 77%

Strategies for vegetative propagation and viral cleaning of a miniature ornamental pineapple hybrid

Silva

Souza

Oliveira

et al. 2021

Acta Sci. Biol. Sci.

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“…Traditional methods of breeding blackberries in the Altai territory are limited by climatic features, so the use of alternative methods of production of planting material of blackberries is relevant. Clonal micropropagation of blackberries requires optimization of all stages of in vitro cultivation for each particular variety, as noted in many authors (Skovorodnikov et al, 2015;Pelizza et al, 2016;Muratova, 2017;Ivanova-Hanina, 2014, 2018Makarov, 2019;Kefayeti et al, 2019). The purpose of our work was to optimize the clonal micropropagation of blackberries.…”

Section: Introductionmentioning

confidence: 91%

Оptimization of blackberry clonal micropropagation

Borodulina¹,

Плаксина²,

Panasenko³

et al. 2019

Ukr J Ecol

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One of the modern methods widely used in the propagation of various garden crops is clonal micropropagation. Research in this direction is ongoing to optimize each stage of reproduction. The paper presents the results of a study of the clonal micropropagation of blackberry varieties Brzezina of Polish selection. For clonal micropropagation, sterile BlackBerry microshoots were used. In order to induce adventitious and axillary shoot formation, we tested various variants of culture medium: hormone-free medium, mediumsa with growth regulators (6-benzylaminopurine, β-indolyl-3-butyric acid, gibberellic acid). It was revealed that blackberry microshoots can take root on medium without growth regulators, but the resulting roots are thin, do not have root hairs. Such microshoots does not adapt to ex vitro conditions. A high reproduction rate (96%) was observed when rooting blackberry microshoots in a culture medium ½ MS + 2.0 μM IBA. Adaptation of regenerated plants to ex vitro conditions is more effective (97% survival rate) on a sand + vermiculite substrate (3: 1).

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Study of the physicochemical characteristics, antimicrobial activity, and in vitro multiplication of wild blackberry species from the Peruvian highlands

Lapiz-Culqui,

Meléndez-Mori,

Tejada-Alvarado

et al. 2024

Sci Rep

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The Peruvian Andes are the natural habitat of several wild blackberry species that are little known and exploited due to the lack of technological and scientific development to support their agricultural potential. In this context, a study was conducted to understand the physicochemical composition, bioactive compounds, antimicrobial activity, and in vitro multiplication of four wild blackberry (Rubus sp.) species from the northern Peruvian highlands. The results indicate that fruits of R. floribundus presented the highest content of total soluble solids (9.58 ± 1.83°Brix) and titratable acidity (1.88 ± 0.07% citric acid). The fruits of R. weberbaueri recorded the highest total phenolic content (415.06 ± 8.69 mg GAE/100 g Ff). The antioxidant capacity determined by the DPPH assay varied significantly among species, with the highest value found in fruits of R. andicola (50.27 ± 0.11 mg TE/100 g Ff). The fruit extracts of R. weberbaueri and R. andicola showed better antimicrobial activity, with Staphylococcus aureus being the most sensitive bacterium. In the in vitro multiplication phase, the results show that BAP (6-Benzylaminopurine) has a significant effect at a dose of 1.5 mg l−1 on shoot number, leaf number, and shoot length. The results may help in the management of genetic resources.

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In vitro establishment of blackberry (Rubus sp.) cultivar 'Xavante'

Abstract: In vitro establishment of blackberry ( Rubus sp.) cultivar 'Xavante'.Ciência Rural, v.46, n.9, set, 2016.

Cited by 3 publications

References 5 publications

Strategies for vegetative propagation and viral cleaning of a miniature ornamental pineapple hybrid

Strategies for vegetative propagation and viral cleaning of a miniature ornamental pineapple hybrid

Оptimization of blackberry clonal micropropagation

Study of the physicochemical characteristics, antimicrobial activity, and in vitro multiplication of wild blackberry species from the Peruvian highlands

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