Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach

Silva, Janaina Lara da; Aparecido, Christiane Ceriani; Hansen, Daiane; Pereira, Tacila Alves Muniz; Felício, Joana D.; Gonçalez, Edlayne

doi:10.1590/0103-8478cr20140581

Cited by 7 publications

(7 citation statements)

References 15 publications

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“…Presence of mycotoxins in food for animal consumption was reported in different regions of Brazil (Sassahara et al, 2003;Gonçalez et al, 2004;Oliveira et al, 2008). A. flavus capable of producing CPA and aflatoxins were isolated from feed samples studied in this work (Silva et al, 2015).…”

Section: Resultsmentioning

confidence: 80%

Monitoring of Mycotoxins in Feed for Goats and Their Residues in Milk

Freitas¹,

Felício²,

Chiebáo³

et al. 2015

JAS

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Aflatoxins are mainly produced by Aspergillus flavus and A. parasiticus, whereas A. flavus, under different conditions, also can produce cyclopiazonic acid (CPA). Several studies on mycotoxins in feed and cow milk have been reported, but the investigation of the co-occurrence of aflatoxins and CPA in feed and goat milk is an unprecedent study in Brazil. This study aimed to evaluate the presence of aflatoxins and cyclopiazonic acid in diets intended for dairy goats and their residues in milk in 10 familiar properties of Southwestern São Paulo region, totalizing 128 samples of feed and 120 samples of milk. Aflatoxins have been extracted and purified in an immunoaffinity column specific for aflatoxins B1 (AFB1), B2 (AFB2), G1 (AFG1) and G2 (AFG2) and aflatoxin M1 (AFM1). The analyses for detection and quantification of toxins were performed by high-performance liquid chromatography. From the analyses of the feeds, 2.34% of these were contaminated with aflatoxin at levels ranging from: 3.65 to 36.93 µg/kg for AFB1; 0.35 to 2.46 µg/kg for AFB2; trace to 46.06 µg/ kg for AFG1 and 0.61 to 1.65 µg/ kg for AFG2. One sample showed a concentration of 87.1 µg/kg, above that allowed by Brazilian law. Concerning CPA, 3.12% of the feed samples were positive for it at concentrations ranging from trace to 1.90 µg/kg. AFM1 and CPA were not detected in the milk samples. The results demonstrate that low contamination by mycotoxins in feeds and the absence of AFM1 and CPA in milk indicate best practices in the management and storage of these feeds.

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Section: Resultsmentioning

confidence: 80%

Monitoring of Mycotoxins in Feed for Goats and Their Residues in Milk

Freitas¹,

Felício²,

Chiebáo³

et al. 2015

JAS

Self Cite

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“…Several Aspergillus strains have been isolated from feeds. For example, Iranian cattle feed [10], poultry feed from South Africa [11], chicken feed from Nigeria [12], and dairy goat feed from Brazil [13].…”

Section: Aflatoxinsmentioning

confidence: 99%

“…Other feed ingredients include distillers dried grains. The components above (especially corn and corn by-products) are the most susceptible to aflatoxigenic fungi attack and therefore aflatoxin contamination [13]. The chemical composition, ingredients and nutritional quality of feed inherently influence the capability of fungi to inoculate and even make use of their genetic machinery available to produce aflatoxins within such a substrate.…”

Section: Feeds and Feed Ingredients And Aflatoxin Contaminationmentioning

confidence: 99%

A Focus on Aflatoxin in Feedstuffs: New Developments in Analysis and Detection, Feed Composition Affecting Toxin Contamination, and Interdisciplinary Approaches to Mitigate It

Granados-Chinchilla¹

2017

Aflatoxin-Control, Analysis, Detection and Health Risks

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Aflatoxins are mold-synthetized secondary metabolites that are capable of causing disease and death in humans and other animals. Aflatoxins hold a prominent place in the discussion on feed safety as are the only mycotoxins with the regulatory framework. Feed ingredients and composition inevitably affect the susceptibility of feed to fungal and toxin contamination. To verify that legal thresholds are being complied, avoiding delivering contaminated feed to animals, and obtain correct prevalence data, analytical methods must be developed which are apt for application on a complex matrix such as animal feed. These methods should include simple screening assays and high-end confirmatory ones. Laboratories without expensive equipment can and should be able to implement methods and to analyze and detect aflatoxins. Aflatoxin contamination is a complex issue that should be assessed interdisciplinarily and farm-to-fork models should be integrated into vigilance. In this chapter, we have devoted some lines to each of the aspects mentioned above focusing on feed aflatoxin contamination.

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“…Poultry feed is compounded from different ingredients, primarily cereals (rice, wheat, barley, oats, rye, corn, sorghum and millet), milling by-products (brans, hulls, pollards) and oil cakes (palm kernel, soybean, sunflower, rapeseed, peanut, linseed, cottonseed). These components (especially corn and corn by-products) are highly susceptible to fungal contamination and thus susceptible to mycotoxin contamination [ 1 ]. Mycotoxins are fungal metabolites produced by fungi of different genera, for example certain Aspergillus species produces aflatoxins (AF) [ 2 ], while certain Fusarium species produce zeralenone (ZEN) [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Trichothecene mycotoxins, including deoxynivalenol (DON), are produced by several fungal genera such as Trichothecium , Stachybotrys , Myrothecium , Cephalosporium , Trichoderma , Penicillium and Fusarium species [ 3 , 4 ]. The chemical composition, ingredients and nutritional quality of poultry feeds influence the growth of mycotoxin producing fungi [ 1 ]. High moisture and crude fat were reported to increase the mycotoxin content of feed [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Reduction of the Adverse Impacts of Fungal Mycotoxin on Proximate Composition of Feed and Growth Performance in Broilers by Combined Adsorbents

Mgbeahuruike

Ejiofor

Ashang

et al. 2021

Toxins

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Synergistic interaction of adsorbents in reducing the adverse impacts of mycotoxin on performance and proximate composition of broiler feeds was investigated. Fungal growth was induced by sprinkling water on the feed. S. cerevisiae + bentonite, kaolin + bentonite or S. cerevisiea + kaolin adsorbent combinations (1.5 g/kg feed) were added and the feeds were stored in black polythene bags. An untreated group was kept as a positive control while fresh uncontaminated feed was used as a negative control. Mycotoxins were extracted from the feeds and quantified using reverse phase HPLC. Proximate composition, nutrient digestibility of the feeds, feed intake and weight gain of the broilers were measured. Deoxynivalenol (DON) concentration in the contaminated/untreated feed was 347 µg/kg while aflatoxin B1 (AFB1) was 34 µg/kg. Addition of bentonite and kaolin in the contaminated feed reduced AFB1 and DON to significantly lower levels. Feed intake and weight gain were low in the broilers fed the contaminated feed. The carbohydrate level was significantly (p < 0.05) reduced from 62.31 to 40.10%, crude protein digestibility dropped from 80.67 to 49.03% in the fresh feed and contaminated feed respectively. Addition of the adsorbents (S. cerevisiae and bentonite) significantly (p < 0.05) improved these parameters.

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Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach

Abstract: Some species of filamentous fungi that infest agricultural commodities are able to produce mycotoxins, contaminating feed and animal products. The aim of this research was to identify the mycoflora present in the feed and forage for dairy goat and to isolate and characterize the

Cited by 7 publications

References 15 publications

Monitoring of Mycotoxins in Feed for Goats and Their Residues in Milk

Monitoring of Mycotoxins in Feed for Goats and Their Residues in Milk

A Focus on Aflatoxin in Feedstuffs: New Developments in Analysis and Detection, Feed Composition Affecting Toxin Contamination, and Interdisciplinary Approaches to Mitigate It

Reduction of the Adverse Impacts of Fungal Mycotoxin on Proximate Composition of Feed and Growth Performance in Broilers by Combined Adsorbents

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