2015
DOI: 10.1590/0103-8478cr20140132
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Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines

Abstract: This study aimed to investigate the genetic variability of two Brazilian free range (Caipira) chickens lines using microsatellites analysis of ten loci. It was collected a total of

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Cited by 3 publications
(3 citation statements)
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“…It has been thought that E. carinicauda would be difficult for us to establish an inbred line because of its more genetic variation, 90 chromosomes [60], and possesses a large complex genome (5.73 Gb) [10]. Therefore, it is necessary to detect the genetic characteristics of inbred line from a relatively low generation to the current generation by SNP and EST-SSR markers [61,62]. In order to obtain accurate estimates within 0.05 of the population allele frequency with high probability (≥95%), a sample size of >30 is often required [63].…”
Section: Discussionmentioning
confidence: 99%
“…It has been thought that E. carinicauda would be difficult for us to establish an inbred line because of its more genetic variation, 90 chromosomes [60], and possesses a large complex genome (5.73 Gb) [10]. Therefore, it is necessary to detect the genetic characteristics of inbred line from a relatively low generation to the current generation by SNP and EST-SSR markers [61,62]. In order to obtain accurate estimates within 0.05 of the population allele frequency with high probability (≥95%), a sample size of >30 is often required [63].…”
Section: Discussionmentioning
confidence: 99%
“…Resultados similares de PIC fueron descritos en poblaciones de gallinas de Sobrarde (0.45) y en líneas japonesas comerciales (0.46), por Monteagudo et al (2011) y Pham et al (2013), respectivamente. Sin embargo, fue inferior al reportado en gallinas indígenas de Rwanda (0.64) por Habimana et al (2020), en poblaciones comerciales coreanas (0.68) por Choi et al, 2015 y en líneas braileñas Paraíso Pedrês (0.79) y Rubro Negra (0.76) por Possamai et al, 2015. Las variaciones podrían atribuirse al tamaño muestral y a la clase de marcadores utilizados, teniendo en cuenta que en la investigación desarrolla en gallinas brasileñas, se amplificaron microsatélites diferentes al de este trabajo, demostrando valores muy superiores, indicando quizás, que dicho panel resultará más informativo en líneas seleccionadas para características productivas.…”
Section: Resultados Y Discusiónunclassified
“…Representando los marcadores microsatélites los de elección, debido a su elevado polimorfismo, presentan herencia codominante y se localizan a lo largo del genoma (Tautz, 1989), permitiendo identificar poblaciones con diversidad genética reducida y más vulnerables a un posible cambio ambiental y distinguir subpoblaciones genéticamente diferenciadas del resto para dirigir esfuerzos de conservación hacia éstas (González, 2003). Diversas investigaciones se han desarrollado utilizando marcadores microsatélites, en gallinas indígenas o nativas (Nxumalo et al, 2020;Mudacheyi et al, 2007), locales (Toalombo et al, 2019;Abebe, Mikko & Johansson, 2015;Ceccobelli et al, 2015;Huo et al, 2014) y líneas comerciales (Choi et al, 2015;Pham et al, 2013;Possamai et al, 2015;Tadano, 2007).…”
Section: Introductionunclassified