Effect of Using Biomimetic Analogs on Dentin Remineralization with Bioactive Cements

Abstract

This study evaluated the impact of using biomimetic analogs (poly-acrylic acid and sodium tri-meta-phosphate) on dentin remineralization using two cement materials, the first is calcium silicate based and the second is calcium hydroxide based materials. Two standardized occlusal cavities (mesial and distal) were prepared within dentin after removal of occlusal enamel. Artificial demineralized dentin was induced through pH cycling (8 h in demineralizing and 16 h in remineralizing solutions). Demineralized cavities were divided into four groups; two groups received cement materials. The other groups were first treated with biomimetic analogs then restored with pulp cement materials. Teeth were sectioned buccolingually into two halves. Treated cavities with analogs were stored in simulated body fluid containing poly-acrylic acid. Untreated cavities were stored in simulated body fluid only. Ground unstained sections of demineralized dentin were examined using light microscope. Specimens were examined after 1, 6 and 12 weeks of storage using energy dispersive X-ray Spectroscopy (EDX) and Vickers microhardness was evaluated. Two-way ANOVA was used to analyze data statistically. Calcium silicate-based cement group with biomimetic analogs showed the highest statistically significant calcium and phosphorous wt% in addition to highest surface hardness values after 12 weeks of storage. Demineralized dentin ground sections showed increase in light zones after total period of storage. Calcium silicate-based cement showed the best ability to enrich the artificial carious dentin with ions for remineralization. Using biomimetic analogs had a significant impact on demineralized dentin surface hardness improvement.

The Synergetic Effect of Silver Diamine Fluoride with Potassium Iodide and Grape Seed Extract on Dentin Remineralization

“…By progression of caries, the organic component of dentin is degraded by some bacterial toxins and intrinsic protease system (2,3) . All these alterations lead to a decrease in the microhardness value of dentin (4) . Dental caries is a dynamic process of demineralization and remineralization, so one of the most important approaches regarding preventive therapies for caries is to enhance the remineralization process.…”

“…Contemporary minimal invasive dentistry strategy is increasingly directed toward; removal of the infected and non remineralizable outer layer of carious dentin and remineralization of the affected carious one using either solution chemistry or more essential tissue regeneration strategies (4) .…”

The top 100 most cited papers in the first 100 years of calcium hydroxide as a dental material (1920-2020): a bibliometric analysis

“…In consequence, most of the papers used calcium hydroxide mainly as an intracanal dressing, pulp-capping agent, chemical component of root canal sealer, and pulp dressing agent for pulpotomy. In the past half century, the incorporation of new scientific knowledge on the protection of the dentine-pulp complex (da Rosa et al, 2019;da Rosa et al, 2018), such as approaches to the preservation of caries-affected dentine (Hernández et al, 2014), the biomimetic aspects of restorative dental biomaterials (Zafar et al, 2020), and the development of new products such as calcium silicate-based materials (Sadoon et al, 2020), has occurred at a staggering pace, promoting a revolution in the field of new functional repair materials and pulp regeneration approaches in the 100 years since the introduction of calcium hydroxide in both adult and Pediatric Dentistry.…”

Comparative evaluation of four different remineralizing agents on dentin surface topography: an invitro study

“…ESEM has been used in many previous investigations for the effect of desensitizing toothpaste on dentin tubule diameter and occlusion (15) . ESEM was used in the study for qualitative assessment and ultramorphological examination for the treated surfaces of dentin (16) .…”