Cytotoxicity Evaluation of Two Bis-Acryl Composite Resins Using Human Gingival Fibroblasts

Abstract

Bis-acryl resins are used for temporary dental restorations and have shown advantages over other materials. The aim of this work was to evaluate the in vitro cytotoxicity of two bis-acryl composite resins (Protemp 4 and Luxatemp Star), obtained at 1, 7 and 40 days after mixing the resin components, using a standardized assay employing human primary cells closely related to oral tissues. Human gingival fibroblast cell cultures were exposed for 24 h to either bis-acryl composite resins, polystyrene beads (negative control) and latex (positive control) extracts obtained after incubation by the different periods, at 37 °C under 5% CO2. Cell viability was evaluated using a multiparametric procedure involving sequential assessment (using the same cells) of mitochondrial activity (XTT assay), membrane integrity (neutral red test) and total cell density (crystal violet dye exclusion test). The cells exposed to the resin extracts showed cell viability indexes exceeding 75% after 24 h. Even when cells were exposed to extracts prepared with longer conditioning times, the bis-acryl composite resins showed no significant cytotoxic effects (p>0.05), compared to the control group or in relation to the first 24 h of contact with the products. There were no differences among the results obtained for the bis-acryl composite resins evaluated 24 h, 7 days and 40 days after mixing. It may be concluded that the bis-acryl resins Protemp 4 and Luxatemp Star were cytocompatible with human gingival fibroblasts, suggesting that both materials are suitable for use in contact with human tissues.

Effects of New Generation All‐Ceramic and Provisional Materials on Fibroblast Cells

“…Their study proved that these materials have no cytotoxic effects on human gingival fibroblasts. 46 These findings agree with the results of this study. According to our results, PT presents high cell viability and low apoptosis rate.…”

“…Similar to this result, Gonçalves et al investigated cytotoxicity of two bis‐acryl composite resins (Protemp 4, Luxatemp Star) using human gingival fibroblasts. Their study proved that these materials have no cytotoxic effects on human gingival fibroblasts . These findings agree with the results of this study.…”

A Review on the Biocompatibility of PMMA-Based Dental Materials for Interim Prosthetic Restorations with a Glimpse into Their Modern Manufacturing Techniques

“…The complex interactions between provisional prosthetic materials and soft oral tissues, namely, the gingival epithelium and oral mucosa, represent extremely important aspects in maintaining a favorable equilibrium of the oral environment. The health of the gingiva located near a provisional crown is influenced by several factors, among which, the material’s characteristics/properties are of utmost importance [ 5 , 18 , 19 , 20 ].…”

BiodentineTM is cytocompatible with human primary osteoblasts

“…In this regard, recent studies have offered suggestions on how to simulate this process, to some extent, including the continuous washing of test material during longer extraction times. 28 Therefore, it is possible that the reduction in cell metabolism induced by Biodentine at 42 days, detected in the present study by the XTT assay, might be related to an overestimation induced by the restrictive culture conditions of the assay.…”