Evaluation of Cellular Proliferative Activity in Patients with Oral Lichen Planus and Hepatitis C through AgNOR Method

Carli, João Paulo De; Silva, Soluete Oliveira da; Linden, Maria Salete Sandini; Busin, Carmen Silvia; Paranhos, Luiz Renato; Souza, Paulo Henrique Couto

doi:10.1590/0103-6440201302379

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…According to Taniguchi et al ., increased cellular proliferation is likely to be a secondary phenomenon due to the damage inflicted on keratinocytes by infiltrating mononuclear cells in the submucosa. [ 28 29 ] However, Coleman A et al . analyzed AgNOR counts in odontogenic cysts and ameloblastomas and came to the conclusion that the AgNOR count may not be a good indicator of cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

A comparison of proliferative capacity of reticular and erosive variants of oral lichen planus by argyrophilic nucleolar organizer regions method

Gotmare¹,

Gupta²,

Waghmare³

et al. 2023

J Microsc Ultrastruct

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Background: Oral lichen planus (OLP), an immune mediated disorder, has been recognized since 1869 and is presented as any one of the six variants. Reticular and erosive are the most frequently encountered. Its proliferative capacity can give some information regarding its progression. We adopted the argyrophilic nucleolar organizer regions (AgNORs) method because of its simplicity to use and dependable results. We evaluated AgNORs in basal, suprabasal, and squamous cell layers. We also compared these three layers within two variants, reticular, and erosive. Materials and Methods: Thirty clinically diagnosed patients of OLP were included in the study. Reticular and erosive variants were included in our study. This was followed by hematoxylin and eosin staining and later by the AgNOR method. The mean number of AgNORs per nucleus was calculated. Results: Thirteen males and 17 females were the gender distribution. Twenty-three (76.67%) had reticular pattern and seven (23.33%) had erosive pattern. The basal cell layer had the highest mean AgNOR compared to suprabasal and squamous layers. Even among, erosive and reticular variants, the former had higher mean AgNOR counts. Discussion: Our results suggest that the inflammatory infiltrate close to the epithelial cells can alter the proliferation index for the pattern of protein synthesis of these cells. Moreover, the high proliferative index in OLP can be related to a specific immunologic response. Conclusion: We conclude that AgNOR can be used as a proliferative marker in earlier lesions to detect the severity.

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Section: Discussionmentioning

confidence: 99%

A comparison of proliferative capacity of reticular and erosive variants of oral lichen planus by argyrophilic nucleolar organizer regions method

Gotmare¹,

Gupta²,

Waghmare³

et al. 2023

J Microsc Ultrastruct

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“…Apremilast may represent a valuable immunomodulatory option for MLP, with an acceptable safety profile and good tolerability in the literature (30)(31)(32)(33). Its effectiveness in other ulcerative mucosal conditions may represent a possible emerging option in MLP.…”

Section: Discussionmentioning

confidence: 99%

Systemic Medical and Procedural Therapies for Mucosal Lichen Planus: Update and Review of Challenges and Successes

et al. 2021

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Context: Mucosal lichen planus is a chronic inflammatory debilitating condition that commonly affects the oral mucosa, but other sites such as the genital and ocular mucosae, esophagus and ear canal may also be involved, with risk of scarring and functional compromise. Spontaneous remission is rare and the condition tends to be recalcitrant to therapy, which makes management challenging. Topical therapies are often ineffective in severe cases and official treatment guidelines including systemic agents and procedural interventions are currently not available. Methods: We performed a literature review on current systemic therapies for mucosal lichen planus using PubMed, MEDLINE and Cochrane databases, with 74 publications identified including retrospective and prospective studies, case reports, and clinical trials. Therapies reviewed included biologics, immunosuppressive therapies, immunomodulating and anti-inflammatory drugs, vitamin A derivatives, devices and interventional therapies, and non-prescription drugs. Results: Reported treatments were classified into the following categories: biologics, anti-metabolic immunosuppressives, direct anti-T cell agents, other immunodulatory agents, antibiotics, retinoids, and procedure based interventions. Most studies on mucosal lichen planus are descriptive in nature and demonstrate low levels of evidence. Randomized controlled trials are lacking, which makes it difficult to directly compare different grading scales and outcome. Conclusions: The authors suggest clinical criteria for initiation of therapy in mucosal lichen planus, such as severe disease, lack of response to topical therapy, mucous membranes involvement and involvement of organs at risk of non-function. The authors also propose a stepwise treatment algorithm based on data from this review and their personal experience in an academic tertiary referral center.

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“…Proliferative activity by AgNOR staining has been studied in potentially malignant lesions of the oral mucosa. 12,13,18 Most of the patients addressed in the current study were aged 51 to 60 years when diagnosed with AC. The main factor leading to AC is the exposure to solar radiation for a long period.…”

Section: Jcdpmentioning

confidence: 99%

Evaluation of Cellular Proliferative Activity in Patients with Actinic Cheilitis through Silver-stained Nucleolar Organizer Region Method

Martins

Zoehler

Busin

et al. 2018

The Journal of Contemporary Dental Practice

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Actinic cheilitis (AC) is a lesion potentially malignant that affects the lips after prolonged exposure to solar ultraviolet (UV) radiation. The present study aimed to assess and describe the proliferative cell activity, using silver-stained nucleolar organizer region (AgNOR) quantification proteins, and to investigate the potential associations between AgNORs and the clinical aspects of AC lesions. Cases diagnosed with AC were selected and reviewed from Center of Histopathological Diagnosis of the Institute of Biological Sciences, Passo Fundo University, Brazil. Clinical data including clinical presentation of the patients affected with AC were collected. The AgNOR techniques were performed in all recovered cases. The different microscopic areas of interest were printed with magnification of *1000, and in each case, 200 epithelial cell nuclei were randomly selected. The mean quantity in each nucleus for NORs was recorded. One-way analysis of variance was used for statistical analysis. A total of 22 cases of AC were diagnosed. The patients were aged between 46 and 75 years (mean age: 55 years). Most of the patients affected were males presenting asymptomatic white plaque lesions in the lower lip. The mean value quantified for AgNORs was 2.4 ± 0.63, ranging between 1.49 and 3.82. No statistically significant difference was observed associating the quantity of AgNORs with the clinical aspects collected from the patients (p > 0.05). The present study reports the lack of association between the proliferative cell activity and the clinical aspects observed in patients affected by AC through the quantification of AgNORs. Knowing the potential relation between the clinical aspects of AC and the proliferative cell activity quantified by AgNORs could play a significant role toward the early diagnosis of malignant lesions in the clinical practice. Actinic cheilitis, Proliferative cell activity, Silver-stained nucleolar organizer regions.

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Evaluation of Cellular Proliferative Activity in Patients with Oral Lichen Planus and Hepatitis C through AgNOR Method

Cited by 3 publications

References 24 publications

A comparison of proliferative capacity of reticular and erosive variants of oral lichen planus by argyrophilic nucleolar organizer regions method

A comparison of proliferative capacity of reticular and erosive variants of oral lichen planus by argyrophilic nucleolar organizer regions method

Systemic Medical and Procedural Therapies for Mucosal Lichen Planus: Update and Review of Challenges and Successes

Evaluation of Cellular Proliferative Activity in Patients with Actinic Cheilitis through Silver-stained Nucleolar Organizer Region Method

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