Influence of Implant Surfaces on Osseointegration: A Histomorphometric and Implant Stability Study in Rabbits

Soares, Priscilla Barbosa Ferreira; Moura, Camilla Christian Gomes; Claudino, Marcela; Carvalho, Valessa Florindo; Rocha, Flaviana Soares; Zanetta‐Barbosa, Darceny

doi:10.1590/0103-6440201300411

Cited by 30 publications

(34 citation statements)

References 23 publications

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“…However, this species shows the fewest similarities to human bone, and its small size, which also limits the quantity and size of inserted implants, is a significant disadvantage. [67][68][69][70][71][72] The most common bones for implantation are a miniature pig maxilla [63][64][65][66] and a rabbit femur. 67,69,70,72 The most frequently used experimental animals, their age/weight (according to the primary literature), the bones selected for implantation, and healing time are shown in Table I.…”

Section: Methods Of Implantationmentioning

confidence: 99%

Evaluating the osseointegration of nanostructured titanium implants in animal models: Current experimental methods and perspectives (Review)

et al. 2016

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The aim of this paper is to review the experimental methods currently being used to evaluate the osseointegration of nanostructured titanium implants using animal models. The material modifications are linked to the biocompatibility of various types of oral implants, such as laser-treated, acid-etched, plasma-coated, and sand-blasted surface modifications. The types of implants are reviewed according to their implantation site (endoosseous, subperiosteal, and transosseous implants). The animal species and target bones used in experimental implantology are carefully compared in terms of the ratio of compact to spongy bone. The surgical technique in animal experiments is briefly described, and all phases of the histological evaluation of osseointegration are described in detail, including harvesting tissue samples, processing undemineralized ground sections, and qualitative and quantitative histological assessment of the bone-implant interface. The results of histological staining methods used in implantology are illustrated and compared. A standardized and reproducible technique for stereological quantification of bone-implant contact is proposed and demonstrated. In conclusion, histological evaluation of the experimental osseointegration of dental implants requires careful selection of the experimental animals, bones, and implantation sites. It is also advisable to use larger animal models and older animals with a slower growth rate rather than small or growing experimental animals. Bones with a similar ratio of compact to spongy bone, such as the human maxilla and mandible, are preferred. A number of practical recommendations for the experimental procedures, harvesting of samples, tissue processing, and quantitative histological evaluations are provided.

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Section: Methods Of Implantationmentioning

confidence: 99%

Evaluating the osseointegration of nanostructured titanium implants in animal models: Current experimental methods and perspectives (Review)

et al. 2016

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“…Foi realizado em coelhos New Zealand e seguiu as metodologias de seleção dos animais e procedimentos cirúrgicos encontradas nos estudos mais citados e renomados de implantes com esses animais (SOARES et al, 2015;SARTORETTO et al, 2015;CALVO-GUIRADO et al, 2014;HERRERO-GLIMENT et al, 2013;MATHIEU et al, 2012;STENPORT et al, 2008;PARK, JANG, SUH, 2008, ELIAS et al, 2008CORDIOLI et al, 2000;KLOKKEVOLD et al,1997;JOHANSSON et al,1998 A pesquisa foi realizada em coelhos com peso médio de 4,5kg, tendo de 9 a 12 meses de idade, peso e idade sugeridos em pesquisas publicadas (SOARES et al, 2015;SARTORETTO et al, 2015;CALVO-GUIRADO et al, 2014;HERRERO-GLIMENT et al, 2013;STENPORT et al, 2008;JOHANSSON et al,1998) parada respiratória e colapso vasomotor, mesmo procedimento realizado por Park, Jang, Suh, 2008;Herrero-Climent et al, 2013;Sartoretto et al, 2015;Soares et al, 2015. Entretanto, Stenport et al(2008 em estudo com coelhos, a overdose intravenosa foi realizada com pentobarbitona (100 mg/mL, Apoteksbolaget, Malmö/ Sweden) de 10mL.…”

Section: Meanunclassified

“…Com a conclusão das fases cirúrgicas, as amostras coletadas ficam submersas em formol a 10% até o início dos procedimentos de desidratação das amostras e inclusão em resina, conforme seus protocolos (SOARES et al, 2015;PIATELLI et al, 2012;SOBOL et al, 2011).…”

Section: Preparação Histológicaunclassified

“…Para isso, a resina deve possuir boas propriedades de infiltração com impregnação dentro das amostras, com baixa viscosidade e polimerização em baixas temperaturas, além de ser hidrofóbica após polimerização. A resina acrílica pode ser da marca LR WhiteResin Medium Grade (London Resin Company, Berkshire, UK), utilizada nesta pesquisa e em pesquisas com animais pelos seguintes autores, Soares et al, 2015;Sobol et al, 2011;Oliveira et al, 2009 Sartoretto et al, 2015;Stancari, 2015e 2011. Segundo Soares et al, 2015, e Sartoretto et al, 2015, a espessura da amostra deve ser de aproximadamente 20 a 30µm, sem nenhum tipo de sobreposição da imagem (roscas e osso) quando visto em microscópio.…”

Section: Preparação Histológicaunclassified

“…Segundo Soares et al, 2015, e Sartoretto et al, 2015, a espessura da amostra deve ser de aproximadamente 20 a 30µm, sem nenhum tipo de sobreposição da imagem (roscas e osso) quando visto em microscópio. Espessura a qual é dada através da politriz com uso das lixas de várias granulações (SARTORETTO et al, 2015, SOARES et al, 2015CALVO-GUIRADO et al, 2013;SOBOL et al, 2011;OLIVEIRA et al, 2009;STENPORT et al, 2008).…”

Section: Preparação Histológicaunclassified

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Avaliação histológica e histomorfométrica da energia superficial e molhabilidade em implantes de titânio grau IV e grau V: estudo experimental em coelhos

Miranda¹

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Evaluation of pulsed electromagnetic field protocols in implant osseointegration: in vivo and in vitro study

et al. 2020

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Objectives The present study aims to evaluate two protocols of pulsed electromagnetic field (PEMF) on osseointegration and establish one that addresses ideal parameters for its use in dentistry, especially in the optimization of the implants osseointegration process. Materials and methods Sixty male rats (Wistar) were allocated into three experimental groups: control (GC), test A (GTA, 3 h exposed), and test B (GTB, 1 h exposed). All animals received titanium implants in both tibias, and PEMF application (15 Hz, ± 1 mT, 5 days/week) occurred only in the test groups. They were euthanized at 03, 07, 21, and 45 days after PEMF therapy. Removal torque, histomorphometric measurements, three-dimensional radiographic evaluation, and in vitro biological assay analyses were performed. Results GTB showed better results compared with GTA in removal torque tests, in bone volume and bone mineral density, cell viability, total protein content, and mineralization nodules (p < 0.05). GTA showed better performance in trabecular bone thickness and cell proliferation compared with GTB (p < 0.05), especially at osseointegration early periods. In the histomorphometric analysis and number of trabeculae, there were no differences in the test groups. Conclusion PEMF as a biostimulator was effective in optimizing the events in bone tissue that lead to osseointegration, especially when applied for a shorter time and in the initial periods of bone healing. Clinical relevance The PEMF therapy is an effective alternative method for optimizing bone healing.

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Influence of Implant Surfaces on Osseointegration: A Histomorphometric and Implant Stability Study in Rabbits

Cited by 30 publications

References 23 publications

Evaluating the osseointegration of nanostructured titanium implants in animal models: Current experimental methods and perspectives (Review)

Evaluating the osseointegration of nanostructured titanium implants in animal models: Current experimental methods and perspectives (Review)

Avaliação histológica e histomorfométrica da energia superficial e molhabilidade em implantes de titânio grau IV e grau V: estudo experimental em coelhos

Evaluation of pulsed electromagnetic field protocols in implant osseointegration: in vivo and in vitro study

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