Filling Effectiveness and Dentinal Penetration of Endodontic Sealers: A Stereo and Confocal Laser Scanning Microscopy Study

Silva, Rogério Vieira; Silveira, Frank Ferreira; Horta, Martinho Campolina Rebello; Duarte, Marco Antônio Húngaro; Cavenago, Bruno Cavalini; Morais, Ivaldo Gomes de; Nunes, Eduardo

doi:10.1590/0103-6440201300138

Cited by 24 publications

(22 citation statements)

References 27 publications

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“…The basic requirements for root canal treatment are effective chemomechanical preparation and three-dimensional obturation of the root canal system 1 . The complexity of the root canal system, with isthmuses, ramiﬁcations, and dentinal tubules, makes it impossible to eliminate microorganisms from root canals during preparation 24 . In addition to the routinely used chemical substances and instruments, other technologies have been proposed to promote antimicrobial activity in the root canal system, such as photoactivated disinfection and non-thermal plasma 7 , 15 , 20 , 27 , 28 .…”

Section: Introductionmentioning

confidence: 99%

Effect of photodynamic therapy and non-thermal plasma on root canal filling: analysis of adhesion and sealer penetration

et al. 2017

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Objective The aim of this study was to evaluate the effect of photodynamic therapy (PDT) and non-thermal plasma (NTP) on adhesion and sealer penetration in root canals.Material and Methods Sixty single-rooted premolars were used. The teeth were prepared using a crown-down technique. NaOCl and EDTA were used for irrigation and smear layer removal, respectively. The root canals were divided into three groups: control, PDT, and NTP. After treatments, the roots were filled using gutta-percha and either AH Plus (AHP) or MTA Fillapex (MTAF) sealers. Samples were sectioned at 4, 8, and 12 mm from the apex (1-mm slices)and analyzed by the push-out bond strength test (adhesion) and confocal laser scanning microscopy (sealer penetration). Data were statistically evaluated using Kruskal-Wallis, Dunn’s, and Spearman’s tests.Results Regarding AHP, bond strength was similar in the NTP group and in the control group, but significantly lower in the PDT group. As to MTAF, both therapies showed lower values than the control group. In the confocal analysis of AHP, maximum and mean penetration, and penetrated area were statistically higher in the control group than in the PDT and NTP groups. Penetrated perimeter was similar among groups. Regarding MTAF, all parameters yielded better results in the NTP than in the control group. The PDT and control groups showed similar results except for penetrated area.Conclusion PDT and plasma therapy affected the adhesion and sealer penetration of root canals filled with AH Plus and MTA Fillapex and there is no positive correlation between adhesion and sealer penetration.

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Section: Introductionmentioning

confidence: 99%

Effect of photodynamic therapy and non-thermal plasma on root canal filling: analysis of adhesion and sealer penetration

et al. 2017

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“…Achieving sealer penetration into dentinal tubules is desirable because it improves the adaptation and retention of the root filling and acts like a physical barrier entombing remaining bacteria inside dentinal tubules (7,8). Adequate flowability allows the sealer to fill irregularities, tubules and lateral canals in a better way (9).…”

Section: Introductionmentioning

confidence: 99%

Influence of the Calcium Hydroxide Intracanal Dressing on Dentinal Tubule Penetration of Two Root Canal Sealers

Cruz¹,

Grecca²,

Piasecki³

et al. 2017

Eur Endod J

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Objective: The aim of this study was to evaluate the effect of a calcium hydroxide (CH) dressing on the tubular penetration of two endodontic sealers, AH Plus (Dentsply Maillefer, Ballaigues, Switzerland) and MTA Fillapex (Angelus, Londrina, Brazil). Methods: Seventy-two mandibular premolars with a single root canal were prepared with ProFile.04 rotary instruments (Dentsply Maillefer) and divided into four groups. In two groups, an intracanal CH dressing was placed for 15 days. The obturations were performed with lateral condensation of gutta-percha in combination with one of the tested sealers. The roots were transversely sectioned at the apical and middle levels. The percentage of sealer penetration in the root canal walls and the percentage of impregnated dentin area in the transverse sections were obtained using confocal laser scanning microscopy. Statistical analysis was performed using one-way analysis of variance (ANOVA) and Games-Howell test. Results: The CH dressing reduced the mean value of tubular penetration in the middle third of teeth obturated with AH Plus (P<0.01), whereas no difference was observed at the apical sections for both sealers. Conclusion:The CH dressing did not interfere with the apical penetration of both tested sealers, however, decreased the tubular penetration in the middle third of the AH Plus root canal fillings. Overall, MTA Fillapex presented higher tubular penetration than AH Plus obturations. Keywords: Calcium hydroxide, confocal laser scanning microscopy, epoxy resin-based root canal sealer, mineral trioxide aggregate-based sealer

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“…Zinc and oxide and eugenol based sealers are still widely employed due to its low cost and acceptable physical/chemical and biological properties (4)(5), while AH Plus presents better behavior and has been recognized as a gold standard amongst endodontic sealers, considering its sealing capacity, physical/chemical and biological properties (5)(6). Among the silicone-based sealers, GuttaFlow was developed to enhance physical characteristics, such as porosity, flow and adhesion to the root canal walls (7)(8).…”

Section: Introductionmentioning

confidence: 99%

Biological Tissue Response to a New Formulation of a Silicone Based Endodontic Sealer

et al. 2016

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Satisfactory biological behavior is a necessary requirement for clinical application of endodontic materials. In this study, the connective tissue responses to silicone (GuttaFlow 2), epoxy resin (AH Plus) and zinc oxide and eugenol (Endofill) based sealers were compared. Twelve Wistar rats had polyethylene tubes (four per animal) containing one of the tested sealers and empty tubes (negative control) implanted in their subcutaneous tissue. The tubes were randomly placed 2 cm from the spine and at least 2 cm apart from one another. Tissue samples with implants were processed for histological analysis after 7 or 60 days (n=6 animals per period). Inflammatory cells, fibrous condensation and abscess were scored according to their intensity. Friedman, followed by Dunn's post hoc, was used to compare sealers. Differences between the two experimental periods were verified using Mann-Witney U test (p<0.05). At 7 days, most of the histological parameters showed no significant differences amongst groups. Endofill group scored higher than the others for giant cells (o<0.05) and promoted a greater number of samples presenting abscess formation. GuttaFlow 2 tended to show a less intense inflammatory infiltrate compared to the other materials. At 60 days, there were no significant differences between groups in most of the histological parameters evaluated. However, it was observed that Endofill scored higher for macrophages (p<0.05) compared to the control group, and GuttaFlow 2 tended to present lower scores than the others for neutrophils and abscess. GuttaFlow 2 showed proper biological behavior and should be considered adequate for clinical practice.

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Filling Effectiveness and Dentinal Penetration of Endodontic Sealers: A Stereo and Confocal Laser Scanning Microscopy Study

Cited by 24 publications

References 27 publications

Effect of photodynamic therapy and non-thermal plasma on root canal filling: analysis of adhesion and sealer penetration

Effect of photodynamic therapy and non-thermal plasma on root canal filling: analysis of adhesion and sealer penetration

Influence of the Calcium Hydroxide Intracanal Dressing on Dentinal Tubule Penetration of Two Root Canal Sealers

Biological Tissue Response to a New Formulation of a Silicone Based Endodontic Sealer

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