Cyrtopodium paludicolum germination with two Tulasnella isolates

Carvalho, Otieres Cirino de; Neto, Vespasiano Borges de Paiva; Padilha, Daly Roxana Castro; Veloso, Tomás Gomes Reis; Bocayuva, Melissa Faust; Soares, Deanna Carla Oliveira; Kasuya, Maria Catarina Megumi

doi:10.1590/0102-33062017abb0181

Cited by 7 publications

(4 citation statements)

References 32 publications

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“…In the germination of C. haagii (Table 1), the highest percentages of germinated seeds were obtained in the OMA medium combined with FHC3E (OMA + FHC3E), B&G + FHC, B&G + FHC3E and asymbiotic B&G, all these with germination values over 60 %. The values obtained for the best treatments are higher than those described by Carvalho et al (2018), who evaluated the percentage of in vitro symbiotic germination for the C. paludicolum orchid in association with mycorrhizal fungi, collected in the same geographic region, reaching 52.89 % of maximum germination.…”

Section: Resultscontrasting

confidence: 60%

Precocious flowering of plants resulting from in vitro germination of Cycnoches haagii seeds on mycorrhizal fungi presence

Torrezan

Silva

Neto

et al. 2018

Pesqui. Agropecu. Trop.

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Cycnoches haagii is an epiphytic Orchidaceae found in the Brazilian Savannah that presents a great ornamental potential. Aiming at developing a complete seminal propagation protocol of C. haagii, as well as testing the influence of the mycorrhizal fungi presence in the process, mature seeds were germinated in vitro in the presence or absence of two fungal isolates, both belonging to the Tulasnella genus (FCH and FHC3E), in three culture media (B&G, Knudson and OMA). The percentage of germinated seeds was evaluated, and the protocorms of the four media with higher percentages were transferred to a new media, aiming at the conversion into plantlets. The seedlings were evaluated for shoot length, number of leaves, length of the largest root, number of roots and total fresh matter. The B&G medium provided a germination rate higher than 60 %, besides a higher seedling growth, contributing to 100 % of survival in the acclimatization stage, independently of the mycorrhizal fungi presence. However, only the seedlings from the B&G medium inoculated with the isolated FHC presented a fast growth when acclimatized, and flowering at the end of the first year of acclimatization. Therefore, the presence of the mycorrhizal fungus FHC on the B&G medium, during the germination phase, is adequate for the propagation of C. haagii, because it accelerates its development and anticipates the ex vitro flowering.

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Section: Resultscontrasting

confidence: 60%

Precocious flowering of plants resulting from in vitro germination of Cycnoches haagii seeds on mycorrhizal fungi presence

Torrezan

Silva

Neto

et al. 2018

Pesqui. Agropecu. Trop.

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“…According to Table 1 , many orchids are considered generalist—or anecdotally, promiscuous—forming OM with fungi isolated from another orchid species [ 6 , 48 , 56 , 57 , 58 , 59 , 60 ]. For specialists, there are no other options but to use OMF isolated from respective plants [ 61 ].…”

Section: Through the Looking Glass: How Orchids Naturally Establismentioning

confidence: 99%

“…Some species are also very easy to germinate; they germinate in the absence of OMF, even when sown in a symbiotic medium. The rest of the germination assay, such as seed morphological changes, germination percentage, duration of germination and development of protocorms, and growth index, can be conducted like that of asymbiotic germination [ 14 , 26 , 34 , 51 , 60 , 69 ].…”

Section: Through the Looking Glass: How Orchids Naturally Establismentioning

confidence: 99%

In Vitro Symbiotic Germination: A Revitalized Heuristic Approach for Orchid Species Conservation

Pujasatria

Miura

Kaminaka

2020

Plants

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As one of the largest families of flowering plants, Orchidaceae is well-known for its high diversity and complex life cycles. Interestingly, such exquisite plants originate from minute seeds, going through challenges to germinate and establish in nature. Alternatively, orchid utilization as an economically important plant gradually decreases its natural population, therefore, driving the need for conservation. As with any conservation attempts, broad knowledge is required, including the species’ interaction with other organisms. All orchids establish mycorrhizal symbiosis with certain lineages of fungi to germinate naturally. Since the whole in situ study is considerably complex, in vitro symbiotic germination study is a promising alternative. It serves as a tool for extensive studies at morphophysiological and molecular levels. In addition, it provides insights before reintroduction into its natural habitat. Here we reviewed how mycorrhiza contributes to orchid lifecycles, methods to conduct in vitro study, and how it can be utilized for conservation needs.

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“…It could be noted that Brazil has the highest number of published works both on propagation and germplasm conservation, while for some countries only researches referring to propagation of native orchid species are reported and for other countries (Guyana, Paraguay, Trinidad and Tobago, Suriname, Uruguay) there were no registered works in any of the topics. Almeida et al 2017;Caovila et al 2016;Cardoso and Ono 2011;Cavalcante Martini et al 2001;Corrêa Pereira et al 2011;2015;Da Silva et al 2014;Da Silva Júnior et al 2012;De Carvalho et al 2018;De Conti et al 2018;De Melo Ferreira et al 2017;De Menezes Gonçalves et al 2012;Dezan et al 2012;Dignart et al 2009;Dorneles and Trevelin 2011;Endres Júnior et al 2014;Favetta et al 2017;Galdiano Júnior et al 2011;2012a,b;Gomes de Araújo et al 2009;Hosomi et al 2017;Lando et al 2016;Lone et al 2010;Mantovani et al 2016;Massaro et al 2012;Monteiro do Rêgo et al 2009;Moraes et al 2005;Pasqual et al 2011;Pedroso-de-Moraes et al 2012;Pereira et al 2018;Pereira Gomes et al 2015;Prizão et al 2012;Rodrigues et al 2015;Rodrigues Guimarães et al 2013;Sasamori et al 2015;…”

Section: In Vitro Conservation Approaches In Orchidsmentioning

confidence: 99%

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

Dolce

Medina

Terada

et al. 2020

Orchid Biology: Recent Trends &Amp; Challenges

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Orchids are an important part of plant biodiversity on the planet due to their high variability among species and their habitats. South America presents more than thirty percent of all known orchid species, being Colombia, Ecuador, Brazil, Peru, and Bolivia between the richest countries in the world in terms of orchid biodiversity. Nevertheless, concerning to the orchid conservation status, in Colombia precisely orchids occupy the unlucky first place as the plant family with the highest number of threatened species. Similar situation is happening in the rest of the South American countries. The two main threats to orchid survival are both anthropogenic: the first one is deforestation, and the second largest threat to orchids is collection from the wild. One desirable action to safeguard these endangered species is to develop procedures that make possible their massive propagation, which should provide material for the eco-rehabilitation of specimens into their natural habitats, the exchange with other entities, the supply to orchid merchants for avoid extractions of nature, and the availability of material for future research. Likewise, the development of systems that allow the ex situ conservation of orchid germplasm is imperative. This chapter reviews the progresses of different in vitro approaches for orchid propagation and germplasm conservation, safeguarding the genetic biodiversity of these species. Several study cases are presented and described to exemplify the protocols developed in the Botanical Institute of Northeast (UNNE-CONICET) for propagating and long-term storing the germplasm of wild orchids from Argentina (Cattleya lundii, Cohniella cepula, Cohniella jonesiana, Gomesa bifolia, Aa achalensis, Cyrtopodium brandonianum, Cyrtopodium hatschbachii, Habenaria bractescens). Moreover, it has been attempted to put together most of the available literature on in vitro propagation and germplasm conservation for South American orchids using different explants and procedures. There are researches of good scientific quality that even cover critical insights into the physiology and factors affecting growth and development as well as storage of several orchid materials. Anyway, studies are still necessary to cover a mayor number of South American species as well as the use of selected material (clonal) for both propagation and conservation approaches.

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Cyrtopodium paludicolum germination with two Tulasnella isolates

Cited by 7 publications

References 32 publications

Precocious flowering of plants resulting from in vitro germination of Cycnoches haagii seeds on mycorrhizal fungi presence

Precocious flowering of plants resulting from in vitro germination of Cycnoches haagii seeds on mycorrhizal fungi presence

In Vitro Symbiotic Germination: A Revitalized Heuristic Approach for Orchid Species Conservation

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

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